A5043869745- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Microfluidic and Capillary Electrophoresis Applications
- Virus-based gene therapy research
- Hydrogels: synthesis, properties, applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Meat and Animal Product Quality
- Cardiac electrophysiology and arrhythmias
- RNA and protein synthesis mechanisms
- Supramolecular Self-Assembly in Materials
- Additive Manufacturing and 3D Printing Technologies
- Orthodontics and Dentofacial Orthopedics
- Analytical Chemistry and Sensors
- Photochromic and Fluorescence Chemistry
- Dental Radiography and Imaging
- Immunotherapy and Immune Responses
- Polymer Surface Interaction Studies
- dental development and anomalies
- Genomics and Chromatin Dynamics
- Ion channel regulation and function
- Antibiotics Pharmacokinetics and Efficacy
Hebrew University of Jerusalem
2008-2024
Jerusalem College of Technology
2020
A method to assemble stimuli-responsive nucleic acid-based hydrogel-stabilized microcapsule-in-microcapsule systems is introduced. An inner aqueous compartment stabilized by a hydrogel-layer (∼150 nm) provides the microcapsule (diameter ∼2.5 μm). The separated from an outer hydrogel layer (thickness of ∼150 that yields system. Different loads, e.g., tetramethyl rhodamine-dextran (TMR-D) and CdSe/ZnS quantum dots (QDs), are loaded in compartments. layers exist higher stiffness state prevents...
UiO-66 metal–organic framework nanoparticles (NMOFs) gated by aptamer-functionalized DNA tetrahedra provide superior biomarker-responsive hybrid nano-carriers for biomedical applications.
Biocatalytic control over the stiffness of pH-responsive hydrogels is applied to develop shape-memory, self-healing and controlled release matrices.
The assembly of adenosine triphosphate (ATP)-responsive and miRNA-responsive DNA tetrahedra-functionalized carboxymethyl cellulose hydrogel microcapsules is presented. are loaded with the doxorubicin-dextran drug or CdSe/ZnS quantum dots as a model. Selective unlocking respective release loads in presence ATP miRNA-141 demonstrated. Functionalization corona tetrahedra nanostructures yields microcarriers that revealed superior permeation into cells. This demonstrated by effective MDA-MB-231...
To evaluate the reliability of a new technique for measuring 3D-scanned orthodontic cast models with cross-section planes using teledent, software, developed at Technion - purpose this research.Thirty plaster were divided into three equal groups according to severity teeth crowding. Measurements mesio-distal tooth width and arch length performed manually on casts conventional caliper. Thereafter, scanned processed software 3-D scanner holographic sensor 'ConoProbe' (by Optimet, Jerusalem,...
Three different topological barriers to switch transcription machineries were introduced including Sr 2+ -ion stabilized G-quadruplex units, T-A·T triplex structures and photoisomerizable azobenzene-nucleic acid blockers.
Biomolecule-loaded nucleic acid-functionalized carboxymethyl cellulose hydrogel-stabilized microcapsules (diameter ca. 2 μm) are introduced as cell-like containments. The loaded with two DNA tetrahedra, T1 and T2, functionalized guanosine-rich G-quadruplex subunits, and/or native enzymes (glucose oxidase, GOx, β-galactosidase, β-gal). In the presence of K+-ions hemin, T1/T2 tetrahedra constituents, in microcapsules, assemble into a hemin/G-quadruplex bridged dimer DNAzyme catalyzing...
The combination of gene therapy and immunotherapy concepts, along recent advances in DNA nanotechnology, have the potential to provide important tools for cancer therapies. We present development stimuli-responsive microcapsules, loaded with a viral immunogenetic agent, harnessing immune response against Coronavirus Disease 2019, COVID-19, selectively attack liver cells (hepatoma) or recognize breast hepatoma, by expression green fluorescence protein, GFP. pH-responsive modified...
A method to assemble loaded stimuli-responsive DNA-polyacrylamide hydrogel-stabilized microcapsules is presented. The involves coating substrate-loaded CaCO3 microparticles, functionalized with nucleic acid promoter units, and cross-linking DNA-modified polyacrylamide chains on the microcapsules, using hybridization chain reaction (HCR) yield DNA-cross-linked hydrogel coating. Dissolution of particles generated hydrogel-protected microcapsules. microcapsule–hydrogel shells include engineered...
Inspired by nature, where dynamic networks control the levels of gene expression and activities transcribed/translated proteins, we introduce nucleic acid-based constitutional (CDNs) as functional modules mimicking native circuits demonstrating CDNs-guided programmed synthesis genes, controlled transcription RNAs, dictated transcription/translation proteins. An auxiliary CDN consisting four dynamically equilibrated constituents AA′, AB′, BA′, BB′ is orthogonally triggered two different...
Vascularized model of human kidney on chip utilizes tissue-embedded sensors to reveal the metabolic signature aminoglycosides toxicity. Data shows gentamicin-induced injury is driven lipotoxicity, which can be blocked by empagliflozin.
Abstract The combination of gene therapy and immunotherapy concepts, along recent advances in DNA nanotechnology, have the potential to provide important tools for cancer therapies. We present development stimuli‐responsive microcapsules, loaded with a viral immunogenetic agent, harnessing immune response against Coronavirus Disease 2019, COVID‐19, selectively attack liver cells (hepatoma) or recognize breast hepatoma, by expression green fluorescence protein, GFP. pH‐responsive modified...
The escalating global demand for meat products has intensified ecological concerns, underscoring the need sustainable alternatives. Although current methods effectively imitate ground meat, mimicking whole cuts, which constitute 54% of market, remains challenging due to lack scalable technology. Injection molding is a massively manufacturing technology developed polymer industry. Here, we introduce two injectable metamaterials: thermally irreversible fat composite named proteoleogel, and...