A5021049281- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Parvovirus B19 Infection Studies
- Genomics and Phylogenetic Studies
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Recycling and Waste Management Techniques
- Healthcare and Environmental Waste Management
- Advanced biosensing and bioanalysis techniques
- Genomics and Rare Diseases
- Genomic variations and chromosomal abnormalities
Hadassah Medical Center
2020-2024
Bar-Ilan University
2020-2023
Pooling multiple swab samples before RNA extraction and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis has been proposed as a strategy to reduce costs increase throughput of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests. However, reports on practical large-scale group testing for SARS-CoV-2 have scant. Key open questions concern reduced sensitivity due sample dilution, the rate false positives, actual efficiency (number tests saved by...
Abstract Congenital cytomegalovirus (cCMV) is the most common intrauterine infection, leading to neurodevelopmental disabilities. Universal newborn infant screening of cCMV has been increasingly advocated. In absence a high-throughput test, which can identify all infected infants, development an accurate and efficient testing strategy remained ongoing challenge. Here we assessed implementation pooled saliva polymerase chain reaction (PCR) tests for universal cCMV, in two hospitals Jerusalem...
Abstract Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control COVID-19 pandemic. Due overwhelming use of RT-PCR tests worldwide, availability test kits has become major bottleneck. Here we demonstrate pooling strategies perform RNA extraction and pools, significantly increasing throughput while maintaining clinical sensitivity. We implemented method routine diagnosis setting asymptomatic populations, already tested 5,464 individuals...
Abstract Pooling multiple swab samples prior to RNA extraction and RT-PCR analysis was proposed as a strategy reduce costs increase throughput of SARS-CoV-2 tests. However, reports on practical large-scale group testing for have been scant. Key open questions concern reduced sensitivity due sample dilution; the rate false positives; actual efficiency (number tests saved by pooling) impact infection in population assay performance. Here we report 133,816 collected at April-September 2020,...
Abstract During the first wave of COVID‐19 worldwide pandemic, number testing in Israel was severely affected by shortage reagents and consumables required to operate instrument performing sample extraction before reverse transcription‐polymerase chain reaction (RT‐PCR), namely QIAsymphony (Qiagen, Hilden, Germany). We aimed develop an assay clean used plastic ware parts while maintaining reliability reproducibility RT‐PCR testing. Wells, rods cover, tips were washed using distilled water,...