A5022415049- Regulation of Appetite and Obesity
- Growth Hormone and Insulin-like Growth Factors
- Biochemical Analysis and Sensing Techniques
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Pancreatic function and diabetes
- Birth, Development, and Health
- Animal Nutrition and Physiology
- Aquaculture Nutrition and Growth
- Phytase and its Applications
- Metabolism, Diabetes, and Cancer
- Digestive system and related health
- Hypothalamic control of reproductive hormones
- Cytokine Signaling Pathways and Interactions
- Infant Nutrition and Health
- Enzyme function and inhibition
- Peptidase Inhibition and Analysis
- Aquaculture disease management and microbiota
- Meat and Animal Product Quality
- Monoclonal and Polyclonal Antibodies Research
- Neurobiology and Insect Physiology Research
Hebrew University of Jerusalem
2013-2024
Hadassah Medical Center
2020
Smith Institute
2014
Ministry of Agriculture and Rural Development
2009
University of Auckland
2008
Génétique Animale et Biologie Intégrative
2007
Agricultural Research Organization
1972-2001
Institut National de la Recherche Agronomique
1994
University of Michigan
1992
University of Manitoba
1985-1986
Metabolic syndrome, leaky guts, and infection syndrome often accompanies obesity hyperglycemia is associated with a breakdown in the integrity of intestinal barrier increased risk systemic infection. Thaiss et al. found that mice Salmonella analog, Citrobacter rodentium , also exhibited hyperglycemia. Deletion glucose transporter GLUT2 altered sensitivity to chemically induced epithelial permeability protected from pathogen invasion. The authors correlation humans between glycated hemoglobin...
An adverse prenatal environment may induce long-term metabolic consequences, in particular obesity and insulin resistance. Although the mechanisms are unclear, this programming has generally been considered an irreversible change developmental trajectory. Adult offspring of rats subjected to undernutrition during pregnancy develop obesity, hyperinsulinemia, hyperleptinemia, especially presence a high-fat diet. Reduced locomotor activity hyperphagia contribute increased fat mass. Using model...
A specific leptin RIA was developed to assess concentrations of in ovine plasma, and shown be efficient with bovine caprine plasma. specific, high-affinity antibody generated against recombinant which, when used a competitive RIA, provided valid estimates linearity (r=+0.989-0.998), recovery (102%), repeatability (13%) limit sensitivity (0.83 ng/ml for 100 microl sample size). Serial dilutions five ovine, or plasma samples showed good parallelism the standard curve. comparison this made...
Research Article| May 01 1963 A pure trypsin inhibitor from soya beans Y BIRK; BIRK Search for other works by this author on: This Site PubMed Google Scholar GERTLER; GERTLER S KHALEF Author and article information Publisher: Portland Press Ltd © The Biochemical Society1963 Biochem J (1963) 87 (2): 281–284. https://doi.org/10.1042/bj0870281 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn Email Cite Get Permissions...
Resistin promotes both inflammation and insulin resistance associated with energy homeostasis impairment. However, the resistin receptor molecular mechanisms mediating its effects in hypothalamus, crucial for control, key insulin-sensitive tissues are still unknown. In current study, we report that chronic infusion lateral cerebral ventricle of normal rats markedly affects hypothalamic peripheral responsiveness. Central treatment inhibited insulin-dependent phosphorylation (IR), AKT,...
An adverse prenatal environment may induce long-term metabolic consequences, in particular obesity, hyperleptinemia, insulin resistance, and type 2 diabetes. Although the mechanisms are unclear, this "programming" has generally been considered an irreversible change developmental trajectory. Adult offspring of rats subjected to undernutrition (UN) during pregnancy develop hyperinsulinemia, especially presence a high-fat diet. Using model maternal UN, we have recently shown that neonatal...
Leptin is a pleiotropic hormone acting both centrally and peripherally. It participates in variety of biological processes, including energy metabolism, reproduction, modulation the immune response. So far, structural elements affecting leptin binding to its receptor remain unknown. We employed random mutagenesis leptin, followed by selection high affinity mutants yeast surface display discovered that replacing residue Asp-23 with non-negatively charged amino acid leads dramatically enhanced...
Acetyl-L-alanyl-L-alanyl-L-alanine methyl ester is a highly specific esteratic substrate for elastase. It only slowly hydrolyzed by trypsin and α-chymotrypsin (0.13 1.2%, respectively, of the rate elastase), can be used in crude preparations study proelastase activation. At pH 8.0 25°, K M = 0.43 mM; k cat 73 s −1 independent between 7.5 10.5. Acetyl-L-alanine poor with 153 mM 6.7 .
Leptin has been implicated in the regulation of feed intake, growth, and reproduction. The objective this study was to determine if centrally administered leptin would affect intake secretion growth hormone (GH) luteinizing (LH) ewe lambs. Eighteen lambs were ovariectomized fitted with intracerebroventricular (i.c.v.) cannulae. Lambs randomly assigned receive either a maintenance diet (fed), or that provided 38% requirements (diet-restricted) for 14 weeks. Subsequently, recombinant ovine...
In the present study we developed a model of diet-induced obesity (DIO) in male C57 BL/6J mice using an 8-wk high fat diet. This should better reflect physiology majority human obese patients than mouse genetic models with defects leptin or signaling. At end diet, DIO displayed increased weight (20%) and higher leptin, insulin, glucose, corticosterone plasma levels compared fed standard diet during same period. Moreover, they became resistant to central effect peripheral administration...
Studies were conducted to determine effects of intrauterine administration recombinant ovine interferon τ (IFNτ), placental lactogen (PL), and growth hormone (GH) on endometrial function. In the first study, IFNτ cyclic ewes for one period (Days 11–15) resulted in an interestrous interval (IEI) ∼30 days, whereas two periods 11–15 Days 21–25) extended IEI greater than 50 days. Administration from 11 15 PL or GH 21 25 failed extend more alone. second IFNτ, PL, differentiation function...
The insulin-sensitive glucose transporter Glut4 is expressed in brain areas that regulate energy homeostasis and body adiposity. In contrast with peripheral tissues, however, the impact of insulin on plasma membrane (PM) translocation neurons not known. this study, we examined role two anorexic hormones (leptin insulin) a human neuronal cell line express endogenous leptin receptors. We show both induce to PM cells activate uptake. Wortmannin, specific inhibitor phosphatidylinositol 3-kinase,...
Leptin, a pleiotropic adipokine, is central regulator of appetite and weight key immunomodulatory protein. Although inborn leptin deficiency causes gain, it unclear whether induced in adult wild-type animals would be orexigenic. Previous work with potent competitive antagonist did not induce true metabolic state mice because short circulating half-life. In this study, we increased the half-life by pegylation, which resulted significantly bioavailability retaining antagonistic activity. Mice...
ADVERTISEMENT RETURN TO ISSUEPREVArticleCatalysis by chymotrypsinogen. Demonstration of an acyl-zymogen intermediateArieh Gertler, Kenneth A. Walsh, and Hans NeurathCite this: Biochemistry 1974, 13, 6, 1302–1310Publication Date (Print):March 1, 1974Publication History Published online1 May 2002Published inissue 1 March 1974https://doi.org/10.1021/bi00703a038RIGHTS & PERMISSIONSArticle Views62Altmetric-Citations77LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text...
1. Feeding on a raw soya-bean diet (RSD) increased the levels of trypsin, chymotrypsin and pancreatopeptidase E but decreased level amylase in pancreas chicks as compared to heated (HSD), while supplementation HSD with trypsin inhibitors activity all four enzymes. + caused significant enlargement only slight depression growth rate. 2. Fasting for 24 h previously given RSD enzymes increase was much greater than those HSD. 3. 4 d adapted resulted dramatic inhibition rate, small weight, an...
Adiponectin, an insulin-sensitizing hormone, and resistin, known to promote insulin resistance, constitute a potential link between obesity type 2 diabetes. In addition, fibroblast growth factor (FGF)21 has effects similar those of adiponectin in regulating glucose lipid metabolism sensitivity. However, the interplay adiponectin, FGF21, resistin signaling pathways during onset resistance is unknown. Here, we investigated whether central promotes through impairment FGF21 signaling. We show...