A5050631176- Bone health and osteoporosis research
- Bone Metabolism and Diseases
- Bone health and treatments
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- Cancer Genomics and Diagnostics
- Parathyroid Disorders and Treatments
- Vitamin D Research Studies
- Genetic Associations and Epidemiology
- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Genetic factors in colorectal cancer
- Wnt/β-catenin signaling in development and cancer
- Alkaline Phosphatase Research Studies
- Osteoarthritis Treatment and Mechanisms
- COVID-19 Clinical Research Studies
- Bone and Joint Diseases
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Birth, Development, and Health
- Folate and B Vitamins Research
- Genetic Syndromes and Imprinting
Marqués de Valdecilla University Hospital
2016-2025
Universidad de Cantabria
2016-2025
Instituto de Investigación Marqués de Valdecilla
2016-2025
Centre for Biomedical Network Research on Rare Diseases
2023-2025
Instituto de Salud Carlos III
2024-2025
Instituto de Investigación de Enfermedades Raras
2024
Centro de Investigación Biomédica en Red
2023-2024
University of Toronto
2024
Complejo Hospitalario Universitario de Albacete
2023
Hospital Universitario Infanta Cristina
2023
Methylating the Family Tree DNA sequences show a high level of similarities between humans and ancient hominids but degree to which there are differences methylated regions in their genomes that may explain phenotypic is unclear. Gokhman et al. (p. 523 , published online 17 April) demonstrate naturally degraded cytosines converted thymines can be used reconstruct methylomes. The results suggest methylation bone tissues modern set genes important for limb development.
Aging | doi:10.18632/aging.100742. Steve Horvath, Vei Mah, Ake T. Lu, Jennifer S. Woo, Oi-Wa Choi, Anna J. Jasinska, José A. Riancho, Spencer Tung, Natalie Coles, Jonathan Braun, Harry V. Vinters, L. Stephen Coles
To determine whether a 6-day course of methylprednisolone (MP) improves outcome in patients with severe SARS-CoV‑2 (Corona Virus Disease 2019 [COVID-19]).
Peak bone mass attained after skeletal growth is a major determinant of the risk developing osteoporosis later in life, hence importance nutritional factors that contribute to gain during infancy and adolescence. An adequate supply vitamin D essential for normal homeostasis. This study was undertaken determine what levels are 25-hydroxyvitamin (25(OH)D) may be considered desirable children assess if maintain these throughout year. Vitamin metabolites parathyroid hormone (PTH) serum were...
To determine genome-wide methylation profiles of bone from patients with hip osteoarthritis (OA) and those osteoporotic (OP) fractures.Trabecular pieces were obtained the central part femoral head 27 fractures 26 OA. DNA was isolated, explored Illumina arrays. RNA extracted, pooled, deep-sequenced to obtain whole transcriptome. Differentially methylated regions identified, connections between genes differentially by pathway text-mining analyses.After quality control, 23,367 CpG sites (13,463...
Abstract Sclerostin, encoded by the SOST gene, is a potent inhibitor of bone formation, produced osteocytes, not osteoblasts, but little known about molecular mechanisms controlling its expression. We aimed to test hypothesis that epigenetic mechanisms, specifically DNA methylation, modulate found two CpG-rich regions in SOST: region 1, located proximal promoter, and 2, around exon 1. qMSP pyrosequencing analysis methylation showed 2 was largely methylated all samples analyzed. In contrast,...
In differentiated cells, aging is associated with hypermethylation of DNA regions enriched in repressive histone post-translational modifications. However, the chromatin marks changes methylation adult stem cells during lifetime are still largely unknown. Here, profiling mesenchymal (MSCs) obtained from individuals aged 2 to 92 yr identified 18,735 hypermethylated and 45,407 hypomethylated CpG sites aging. As sequences were marks. Most importantly, strongly active mark H3K4me1 suggesting...
Osteoblasts are specialized cells that form new bone and also indirectly influence resorption by producing factors modulate osteoclast differentiation. Although the methylation of CpG islands plays an important role in regulation gene expression, there is still scanty information about its human bone. The aim this study was to investigate on transcriptional levels two osteoblast-derived critical osteoclastogenesis: receptor activator nuclear factor NF-κB ligand (RANKL) soluble decoy...
ABSTRACT Background We aimed to determine whether a 6-day course of intravenous methylprednisolone (MP) improves outcome in patients with SARS CoV-2 infection at risk developing Acute Respiratory Distress Syndrome (ARDS). Methods Multicentric, partially randomized, preference, open-label trial, including adults COVID-19 pneumonia, impaired gas exchange and biochemical evidence hyper-inflammation. Patients were assigned standard care (SOC), or SOC plus MP [40mg/12h 3 days, then 20mg/12h...
Nitric oxide synthases (NOS) are enzymes that produce nitric (NO) from L-arginine in a reaction yielding citrulline as coproduct. modulates the activity of wide variety cells, but little is known about its effects on bone cells. In present study we report NOS inhibitor NG-monomethyl-L-arginine (NMMA) induced dose-dependent inhibitory effect proliferation osteoblast-like cell lines MG63 and ROS 17/2.8. The was prevented by increasing concentrations medium NO donor sodium nitroprusside....
A collaborative work was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) to estimate Y-STR mutation rates. Seventeen Y chromosome STR loci (DYS19, DYS385, DYS389I II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460, DYS461, DYS635 [GATA C4], GATA H4, A10) were analyzed in a sample of 3,026 father/son pairs. Among 27,029 allele transfers, 54 mutations observed, with an overall rate across 17 1.998 × 10–3 (95% CI, 1.501 2.606 10–3). With just one...
<h3>Objectives</h3> The genetic aetiology of osteoarthritis has not yet been elucidated. To enable a well-powered genome-wide association study (GWAS) for osteoarthritis, the authors have formed arcOGEN Consortium, UK-wide collaborative effort aiming to scan over 7500 cases in two-stage scan. Here report findings stage 1 interim analysis. <h3>Methods</h3> performed knee and hip 3177 4894 population-based controls from UK. Replication promising signals was carried out silico five further...
Insufficient activity of the bone-forming osteoblasts leads to low bone mass and predisposes fragility fractures. The functional capacity human mesenchymal stem cells (hMSCs), precursors osteoblasts, may be compromised in elderly individuals, relation with epigenetic changes associated aging. However, role hMSCs pathogenesis osteoporosis is still unclear. Therefore, we aimed characterize genome-wide methylation gene expression signatures differentiation from patients hip We obtained femoral...