A5073787062- Neuropeptides and Animal Physiology
- Fibroblast Growth Factor Research
- Bone health and treatments
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Receptor Mechanisms and Signaling
- Hypothalamic control of reproductive hormones
- Regulation of Appetite and Obesity
- Ion channel regulation and function
- Protein Kinase Regulation and GTPase Signaling
- Neuroscience of respiration and sleep
- Neuroendocrine Tumor Research Advances
- Pain Mechanisms and Treatments
- Thyroid Disorders and Treatments
- Stress Responses and Cortisol
- Metabolism, Diabetes, and Cancer
- Neurotransmitter Receptor Influence on Behavior
- Hearing, Cochlea, Tinnitus, Genetics
- Diabetes Treatment and Management
- Cell Image Analysis Techniques
- Sleep and Wakefulness Research
- Advanced Fluorescence Microscopy Techniques
- Biotin and Related Studies
- Parathyroid Disorders and Treatments
- S100 Proteins and Annexins
National Institute of Mental Health
2015-2025
National Institutes of Health
2002-2022
Society for Neuroscience
2014
National Cancer Institute
2009
Nagasaki University
2002
National Institute of Neurological Disorders and Stroke
1998
Harvard University
1996
Dana (United States)
1996
Rotterdam University of Applied Sciences
1996
Stanford University
1992
The National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence cDNA clone containing complete ORF for each human mouse gene. ESTs were generated from libraries enriched full-length cDNAs analyzed candidate full-ORF clones, which then sequenced high accuracy. MGC has currently verified the full nonredundant set >9,000 >6,000 genes. Candidate clones an additional 7,800 3,500 genes also have been identified. All sequences...
The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing complete open reading frame (ORF) for every human mouse gene. initially used random strategy select clones from large number libraries diverse tissues. Candidate were chosen based on 5′-EST sequences, then fully sequenced high accuracy analyzed by algorithms developed this project. Currently, more than 11,000 10,000 genes are...
Gastric inhibitory polypeptide (GIP), or glucose-dependent insulinotropic peptide, is released from endocrine cells in the small intestine after meals. It involved several facets of anabolic response and thought to be particularly important stimulating insulin secretion. We have cloned, functionally expressed, mapped distribution receptor for GIP. a member secretin-vasoactive intestinal family G-protein-coupled receptors. When expressed tissue culture cells, it stimulates cAMP production...
Vasoactive intestinal polypeptide (VIP) has a variety of physiological effects. Pharmacological evidence suggesting that VIP acts via multiple receptors been confirmed by the cloning two (VIP1 and VIP2) with very different amino acid sequences. At both VIP1 VIP2 receptor VIP, PHI, PACAP38, PACAP27 have similar potency to each other. Only is activated secretin. The messenger RNAs (mRNAs) for completely distributions as mapped in situ hybridization histochemistry. mRNA predominantly found...
We have identified a G-protein-coupled receptor specifically activated by parathyroid hormone, which we refer to as the PTH2 receptor. Parathyroid hormone (PTH) and hormone-related peptide (PTHrP, hypercalcemia of malignancy factor) activate previously PTH/PTHrP receptor, has widespread tissue distribution. The is much more selective in ligand recognition appears specific It PTH not PTHrP particularly abundant brain pancreas.
The use of different receptor blocking agents and single-unit recording techniques indicates that feedback inhibition brain noradrenaline neurons by tricyclic antidepressants is mediated presynaptic α-receptors. After chronic imipramine treatment, in the locus coeruleus rat remained partly depressed, agreement with clinical data. They were, however, resistant to further or clonidine.
The vesicular acetylcholine transporter (VAChT) has been identified and characterized based on the acquisition of high affinity vesamicol binding proton-dependent, vesamicol-sensitive accumulation by a fibroblast cell line transfected with clone from rat pheochromocytoma cDNA library encoding this protein. distribution VAChT mRNA coincides that reported for choline acetyltransferase (ChAT), enzyme required biosynthesis, in peripheral central cholinergic nervous systems. A human was used to...
Abstract We present Richardson–Lucy network (RLN), a fast and lightweight deep learning method for three-dimensional fluorescence microscopy deconvolution. RLN combines the traditional iteration with fully convolutional structure, establishing connection to image formation process thereby improving performance. Containing only roughly 16,000 parameters, enables four- 50-fold faster processing than purely data-driven networks many more parameters. By visual quantitative analysis, we show that...
A cDNA encoding the dopamine transporter from bovine brain substantia nigra was identified on basis of its structural homology to other, recently cloned, neurotransmitter transporters. The sequence 693-amino acid protein is quite similar those rat gamma-aminobutyric acid, human norepinephrine, and serotonin Dopamine mRNA detected by in situ hybridization but not locus coeruleus, raphe, caudate, or other areas. [3H]Dopamine accumulation tissue culture cells transfected with inhibited...
The tachykinin neuropeptides, substance P and K, are produced in nociceptive primary sensory neurons many brain regions involved pain signaling. However, the precise role importance of these neuropeptides responses has been debated. We now show that mice cannot produce peptides display no significant following formalin injection have an increased threshold hotplate test. On other hand, mutant react normally tail flick assay acetic acid-induced writhing tests. These results demonstrate and/or...
Acetylcholine receptors (AChRs) are packed in the postsynaptic membrane at neuromuscular junctions a density of approximately 20,000/micron 2, whereas few micrometers away is less than 20/micron 2. To understand how this remarkable distribution comes about during nerve-muscle synapse formation, we have attempted to isolate factors from neural tissue that can promote accumulation AChRs and/or alter their distribution. In paper report purification polypeptide chick brains increase rate...
Familial amyotrophic lateral sclerosis (FALS), a degenerative disorder of motor neurons, is associated with mutations in the Cu/Zn superoxide dismutase gene SOD1 some affected families. We confirm recently reported ala4→val mutation exon 1 and report that this both most commonly detected all among clinically severe. By comparision our other FALS families, reduced survival time after onset: 1.2 years, as compared to 2.5 years for patients. also demonstrate prominently expressed normal neurons...
Glucose-dependent insulinotropic polypeptide (GIP) is a 42-amino acid peptide produced by K cells of the mammalian proximal small intestine and potent stimulant insulin release in presence hyperglycemia. However, its relative physiological importance as postprandial agent unknown. Using LGIPR2 stably transfected with rat GIP receptor cDNA, (1-42) stimulation cyclic adenosine monophosphate (cAMP) production was inhibited concentration-dependent manner (7-30)-NH2. Competition binding assays...
Guanosine triphosphate (GTP) and dighosphate nucleotides decrease the binding of agonist ligands [3H]apomorphine [3H]ADTN to dopamine receptors in rat corpus striatum with half maximal reduction at 5 µM. These also reduce inhibition antagonist [3H]spiroperidol without affecting total binding. monophosphate adenine display negligible influence on receptor GTP reduces affinity no effect numbers sites.
Pain and depression are frequently associated with often persist after resolution of an initial injury. Identifying the extent to which remains causally ongoing physical discomfort during chronic pain, or becomes independent it, is important problem for basic neuroscience psychiatry. Difficulty in distinguishing between effects aversive sensory input its long-term consequences a significant roadblock, especially animal models. To address this relationship localized more global consequences,...
Oxytocin is released from neurons in the paraventricular hypothalamic nucleus (PVN) mothers upon suckling and during adult social interactions. However, neuronal pathways that activate oxytocin contexts are not yet established. Neurons posterior intralaminar complex of thalamus (PIL), which contain tuberoinfundibular peptide 39 (TIP39) activated by pup exposure lactating mothers, provide a candidate projection. Innervation TIP39 was examined double labeling combination with electron...
The PTH2 receptor is a recently identified G protein-coupled activated by PTH. Its amino acid sequence most similar to the PTH/PTHrP receptor, but unlike it PTH and not PTH-related peptide. We previously demonstrated using Northern blots that expression of messenger RNA was greatest within brain occurred at lower levels in pancreas, testis, placenta. have now obtained complementary DNA encoding rat used study distribution situ hybridization histochemistry. abundantly expressed arterial...
Complementary DNA clones corresponding to a messenger RNA encoding 56 kDa polypeptide have been obtained from Torpedo marmorata and ocellata electric lobe libraries, by homology screening with probe the putative acetylcholine transporter nematode Caenorhabditis elegans . The proteins display approximately 50% overall identity C. unc‐17 protein 43% two vesicle monoamine transporters (VMAT1 VMAT2). This family of is highly conserved within 12 domains which potentially span membrane, little...
We studied a 45-yr-old woman with food-dependent Cushing's syndrome. Plasma cortisol levels were subnormal (4-47 nmol/L) after an overnight fast and increased mixed meal to values between 500-1000 nmol/L. There was close correlation circulating gastric inhibitory polypeptide (GIP) during normal food intake (r = 0.92; P < 0.0002). corticotropin (ACTH) undetectable. Nonfasting plasma not suppressed by low or high doses of dexamethasone. ACTH did increase human CRH administration, but fasting...