A5056504447- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Electron Microscopy Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Cellular Mechanics and Interactions
- Image Processing Techniques and Applications
- Single-cell and spatial transcriptomics
- Neurobiology and Insect Physiology Research
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Optical Imaging and Spectroscopy Techniques
- Force Microscopy Techniques and Applications
- Digital Holography and Microscopy
- RNA Research and Splicing
- Advanced X-ray Imaging Techniques
- Photoreceptor and optogenetics research
- 3D Printing in Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Near-Field Optical Microscopy
National Institute of Biomedical Imaging and Bioengineering
2016-2025
National Institutes of Health
2016-2025
Marine Biological Laboratory
2016-2025
Janelia Research Campus
2007-2025
Howard Hughes Medical Institute
2007-2025
Advanced Imaging Research (United States)
2017-2025
Optica
2018-2021
University of Maryland, College Park
2008-2021
C4 Imaging (United States)
2021
University of Michigan
2010
Accurate determination of the relative positions proteins within localized regions cell is essential for understanding their biological function. Although fluorescent fusion are targeted with molecular precision, position these genetically expressed reporters usually known only to resolution conventional optics ( approximately 200 nm). Here, we report use two-color photoactivated localization microscopy (PALM) determine ultrastructural relationship between different fused spectrally distinct...
The Escherichia coli chemotaxis network is a model system for biological signal processing. In E. coli, transmembrane receptors responsible transduction assemble into large clusters containing several thousand proteins. These sensory have been observed at cell poles and future division sites. Despite extensive study, it remains unclear how form, what controls cluster size density, the cellular location of robustly maintained in growing dividing cells. Here, we use photoactivated localization...
The Caenorhabditis elegans embryo is a powerful model for studying neural development, but conventional imaging methods are either too slow or phototoxic to take full advantage of this system. To solve these problems, we developed an inverted selective plane illumination microscopy ( i SPIM) module noninvasive high-speed volumetric living samples. SPIM designed as straightforward add-on microscope, permitting mounting specimens and facilitating use by development neurobiology laboratories....
Abstract Subunit vaccines have been investigated in over 1000 clinical trials of cancer immunotherapy, but shown limited efficacy. Nanovaccines may improve efficacy rarely clinically translated. By conjugating molecular with Evans blue (EB) into albumin-binding (AlbiVax), here we develop promising albumin/AlbiVax nanocomplexes that self-assemble vivo from AlbiVax and endogenous albumin for efficient vaccine delivery potent immunotherapy. PET pharmacoimaging, super-resolution microscopies,...
// Mikako Ogawa 1, 7, * , Yusuke Tomita 2, Yuko Nakamura 3 Min-Jung Lee 2 Sunmin Saori Tadanobu Nagaya Kazuhide Sato Toyohiko Yamauchi 4 Hidenao Iwai Abhishek Kumar 5 Timothy Haystead 6 Hari Shroff Peter L. Choyke Jane B. Trepel Hisataka Kobayashi 1 Medical Photonics Research Center, Hamamatsu University School of Medicine, 431-3192, Japan Developmental Therapeutics Branch, Center for Cancer Research, National Institute, NIH, Bethesda, MD 20892, USA Molecular Imaging Program, Central...
Nanomedicines that co-deliver DNA, RNA, and peptide therapeutics are highly desirable yet remain underdeveloped for cancer theranostics. Herein, we report self-assembled intertwining DNA-RNA nanocapsules (iDR-NCs) efficiently delivered synergistic DNA CpG short hairpin RNA (shRNA) adjuvants, as well tumor-specific neoantigens into antigen presenting cells (APCs) in lymph nodes immunotherapy. These nanovaccines were prepared by (1) producing tandem shRNA via concurrent rolling circle...
Abstract We present Richardson–Lucy network (RLN), a fast and lightweight deep learning method for three-dimensional fluorescence microscopy deconvolution. RLN combines the traditional iteration with fully convolutional structure, establishing connection to image formation process thereby improving performance. Containing only roughly 16,000 parameters, enables four- 50-fold faster processing than purely data-driven networks many more parameters. By visual quantitative analysis, we show that...
Abstract The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is limited to ∼300 nm. Here we present two distinct, complementary methods improve in 3D SIM with minimal or no modification the optical system. We show that placing a mirror directly opposite sample enables four-beam interference higher spatial frequency content than illumination, offering near-isotropic imaging ∼120-nm lateral and 160-nm resolution. also developed deep learning method achieving...
Abstract All multicellular systems produce and dynamically regulate extracellular matrices (ECMs) that play essential roles in both biochemical mechanical signaling. Though the spatial arrangement of these assemblies is critical to their biological functions, visualization ECM structure challenging, part because biomolecules compose are difficult fluorescently label individually collectively. Here, we present a cell-impermeable small-molecule fluorophore, termed Rhobo6, turns on red shifts...
Recent advances in optical microscopy have enabled biological imaging beyond the diffraction limit at nanometer resolution. A general feature of most techniques based on photoactivated localization (PALM) or stochastic reconstruction (STORM) has been use thin samples combination with total internal reflection, thus limiting depth to a fraction an wavelength. However, study whole cells organelles that are typically up 15 microm deep into cell, extension these methods three-dimensional (3D)...