A5068937717- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Neurobiology and Insect Physiology Research
- Electron and X-Ray Spectroscopy Techniques
- Physics of Superconductivity and Magnetism
- Quantum and electron transport phenomena
- Force Microscopy Techniques and Applications
- Insect and Arachnid Ecology and Behavior
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Quantum, superfluid, helium dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Atomic and Subatomic Physics Research
- Near-Field Optical Microscopy
- Cold Atom Physics and Bose-Einstein Condensates
- Iron-based superconductors research
- Surface and Thin Film Phenomena
- Cell Image Analysis Techniques
- Cellular Mechanics and Interactions
- Morphological variations and asymmetry
- Magnetic properties of thin films
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Photoreceptor and optogenetics research
Janelia Research Campus
2016-2025
Howard Hughes Medical Institute
2016-2025
Integral Consulting (United States)
2024
Helix (United States)
2015-2023
MED Institute
2010
Millennium Engineering and Integration (United States)
2006
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2006
National High Magnetic Field Laboratory
2006
Florida State University
2006
University of Idaho
2006
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 to 25 nanometers), and then bleached. The aggregate position information from all was assembled into superresolution image. used this method--termed photoactivated localization microscopy--to image specific target in thin sections lysosomes mitochondria; fixed whole cells, we...
Understanding molecular-scale architecture of cells requires determination 3D locations specific proteins with accuracy matching their nanometer-length scale. Existing electron and light microscopy techniques are limited either in molecular specificity or resolution. Here, we introduce interferometric photoactivated localization (iPALM), the combination single-photon, simultaneous multiphase interferometry that provides sub-20-nm protein optimal specificity. We demonstrate measurement 25-nm...
The Abrikosov flux lattice is imaged in Nb${\mathrm{Se}}_{2}$ by tunneling into the superconducting gap edge with a low-temperature scanning-tunneling microscope. conductance single vortex core strongly peaked at Fermi energy, suggesting existence of states or excitations. As one moves away from core, this feature evolves density which consistent BCS gap.
Luminescent centers with sharp (<0.07 millielectron volt), spectrally distinct emission lines were imaged in a GaAs/AIGaAs quantum well by means of low-temperature near-field scanning optical microscopy. Temperature, magnetic field, and linewidth measurements establish that these arise from excitons laterally localized at interface fluctuations. For sufficiently narrow wells, virtually all originates such centers. Near-field microscopy/spectroscopy provides to access energies homogeneous...
Significance The cytoplasm of eukaryotic cells is compartmentalized by intracellular membranes that define subcellular organelles. One these organelles, the endoplasmic reticulum, forms a continuous network tubules and cisternae extends throughout all cell compartments, including neuronal dendrites axons. This communicates with most other organelles vesicular transport, also contacts do not lead to fusion but allow cross-talk between adjacent bilayers. Though membrane have previously been...
The endoplasmic reticulum (ER) is an expansive, membrane-enclosed organelle that plays crucial roles in numerous cellular functions. We used emerging superresolution imaging technologies to clarify the morphology and dynamics of peripheral ER, which contacts modulates most other intracellular organelles. Peripheral components ER have classically been described as comprising both tubules flat sheets. show this system consists almost exclusively at varying densities, including structures we...
The superconducting-vortex-core spectra show a zero-bias peak which splits within coherence length of the core. Further away from core these split peaks merge gradually with gap edge and give direct local measure superfluid velocity. vortex-core states are imaged both at Fermi energy just below to reveal two different sixfold star-shaped structures. anisotropy size images may be consequence crystalline band structure its charge-density-wave as well interaction neighboring vortices Abrikosov...
Understanding memory formation, storage and retrieval requires knowledge of the underlying neuronal circuits. In Drosophila, mushroom body (MB) is major site associative learning. We reconstructed morphologies synaptic connections all 983 neurons within three functional units, or compartments, that compose adult MB's α lobe, using a dataset isotropic 8 nm voxels collected by focused ion-beam milling scanning electron microscopy. found Kenyon cells (KCs), whose sparse activity encodes sensory...
We describe the implementation of a scanning Hall probe microscope outstanding magnetic field sensitivity (∼0.1 G) and unprecedented spatial resolution (∼0.35 μm) to detect surface fields at close proximity sample. Our combines advantages submicron fabricated on GaAs/Al0.3Ga0.7As heterostructure chip tunneling microscopy technique for precise positioning. demonstrate its usefulness by imaging individual vortices in high Tc La1.85Sr0.15CuO4 films superconducting networks, bubble domains.
Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) can automatically generate 3D images with superior z-axis resolution, yielding data that needs minimal image registration and related post-processing. Obstacles blocking wider adoption of FIB-SEM include slow imaging speed lack long-term system stability, which caps the maximum possible acquisition volume. Here, we present techniques accelerate while greatly improving reliability, allowing to operate for months generating continuously...
Optical and electron microscopy have made tremendous inroads toward understanding the complexity of brain. However, optical offers insufficient resolution to reveal subcellular details, lacks throughput molecular contrast visualize specific constituents over millimeter-scale or larger dimensions. We combined expansion lattice light-sheet image nanoscale spatial relationships between proteins across thickness mouse cortex entire Drosophila These included synaptic at dendritic spines,...
Within cells, the spatial compartmentalization of thousands distinct proteins serves a multitude diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) can elucidate protein relationships to global ultrastructure, but has suffered from tradeoffs structure preservation, retention, resolution, field view. We developed platform for three-dimensional cryogenic SR focused ion beam-milled block-face EM across entire vitreously frozen cells. The...
Significance Circuit diagrams of brains are generally reported only as absolute or consensus networks; these fail to identify the accuracy connections, however, for which multiple circuits same neurons must be documented. For this reason, modular composition Drosophila visual system, with many identified neuron classes, is ideal. Using EM, we synaptic connections in fly’s second relay neuropil, medulla, 20 classes a so-called “core connectome,” those present seven neighboring columns. These...
Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study living cells organisms. Unfortunately, applicability microscope limited, since diffraction imposes limitations on spatial resolution image. Consequently details of, for example, cellular protein distributions, can be visualized only certain extent. Fortunately, recent years have witnessed...
A fundamental objective in molecular biology is to understand how DNA organized concert with various proteins, RNA, and biological membranes. Mitochondria maintain express their own (mtDNA), which arranged within structures called nucleoids. Their functions, dimensions, composition, precise locations relative other mitochondrial are poorly defined. Superresolution fluorescence microscopy techniques that exceed the previous limits of imaging small highly compartmentalized mitochondria have...
Microscopic images of specific proteins in their cellular context yield important insights into biological processes and architecture. The advent superresolution optical microscopy techniques provides the possibility to augment EM with nanometer-resolution fluorescence access precise location ultrastructure. Unfortunately, efforts combine have been stymied by divergent incompatible sample preparation protocols two methods. Here, we describe a protocol that preserves both delicate...
Lipid droplets (LDs) are neutral lipid storage organelles that transfer lipids to various including peroxisomes. Here, we show the hereditary spastic paraplegia protein M1 Spastin, a membrane-bound AAA ATPase found on LDs, coordinates fatty acid (FA) trafficking from LDs peroxisomes through two interrelated mechanisms. First, Spastin forms tethering complex with peroxisomal ABCD1 promote LD–peroxisome contact formation. Second, recruits membrane-shaping ESCRT-III proteins IST1 and CHMP1B via...
Advanced FIB-SEM and 3D-SIM imaging uncover cohesin-independent chromatin arrangement into structural functional modules.