A5028623672- Lipid Membrane Structure and Behavior
- Force Microscopy Techniques and Applications
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Advanced Nanomaterials in Catalysis
- Malaria Research and Control
- Supramolecular Self-Assembly in Materials
- Cellular Mechanics and Interactions
- Mosquito-borne diseases and control
- Nanopore and Nanochannel Transport Studies
- Graphene and Nanomaterials Applications
- Mechanical and Optical Resonators
- Theoretical and Computational Physics
- Nanoparticle-Based Drug Delivery
- Luminescence and Fluorescent Materials
- Physics of Superconductivity and Magnetism
- Erythrocyte Function and Pathophysiology
- Monoclonal and Polyclonal Antibodies Research
- Invertebrate Immune Response Mechanisms
- Immunotherapy and Immune Responses
- Biosensors and Analytical Detection
- thermodynamics and calorimetric analyses
- Molecular Junctions and Nanostructures
National Institutes of Health
2015-2025
National Institute of Biomedical Imaging and Bioengineering
2016-2025
University of Tennessee Health Science Center
2024
University of Alberta
2018
Bethesda University
2014-2016
Massachusetts Institute of Technology
2014
Provincial Laboratory of Public Health
2014
United States Department of Health and Human Services
2006
Office of the Director
2003-2006
Office of Research Services
2006
Abstract The physical properties of two-dimensional (2D) extracellular matrices (ECMs) modulate cell adhesion dynamics and motility, but little is known about the roles local microenvironmental differences in three-dimensional (3D) ECMs. Here we generate 3D collagen gels varying matrix microarchitectures to characterize their regulation migration. ECMs containing bundled fibrils demonstrate enhanced adhesion-scale stiffness increased stability through balanced ECM/adhesion coupling, whereas...
Multimodal imaging-guided photothermal therapy (PTT), for the of cancer, based on a ferritin (FRT) nanocage loaded with near-infrared dye IR820 (designated DFRT) is demonstrated. The dual roles DFRT (in imaging and PTT) are successfully balanced by using two different excitation wavelengths: 550 nm high quantum-yield fluorescence one hand 808 photoacoustic PTT conversion efficiency other. As service to our authors readers, this journal provides supporting information supplied authors. Such...
Abstract Subunit vaccines have been investigated in over 1000 clinical trials of cancer immunotherapy, but shown limited efficacy. Nanovaccines may improve efficacy rarely clinically translated. By conjugating molecular with Evans blue (EB) into albumin-binding (AlbiVax), here we develop promising albumin/AlbiVax nanocomplexes that self-assemble vivo from AlbiVax and endogenous albumin for efficient vaccine delivery potent immunotherapy. PET pharmacoimaging, super-resolution microscopies,...
Nanomedicines that co-deliver DNA, RNA, and peptide therapeutics are highly desirable yet remain underdeveloped for cancer theranostics. Herein, we report self-assembled intertwining DNA-RNA nanocapsules (iDR-NCs) efficiently delivered synergistic DNA CpG short hairpin RNA (shRNA) adjuvants, as well tumor-specific neoantigens into antigen presenting cells (APCs) in lymph nodes immunotherapy. These nanovaccines were prepared by (1) producing tandem shRNA via concurrent rolling circle...
The development of smart theranostic systems with favourable biocompatibility, high loading efficiency, excellent circulation stability, potent anti-tumour activity, and multimodal diagnostic functionalities is importance for future clinical application. premature burst release poor degradation kinetics indicative polymer-based nanomedicines remain the major obstacles translation. Herein we prepare shell-crosslinked nanoparticles (SCNPs) using a β-cyclodextrin-based polyrotaxane...
Despite the effort of developing various nanodelivery systems, most them suffer from undesired high uptakes by reticuloendothelial system, such as liver and spleen. Herein we develop an endogenous phosphatase-triggered coassembly strategy to form tumor-specific indocyanine green (ICG)-doped nanofibers (5) for cancer theranostics. Based on coordinated intermolecular interactions, 5 significantly altered near-infrared absorbance ICG, which improves critical photoacoustic photothermal...
2D nanomaterials have attracted broad interest in the field of biomedicine owing to their large surface area, high drug-loading capacity, and excellent photothermal conversion. However, few studies report "enzyme-like" catalytic performance because it is difficult prepare enzymatic nanosheets with small size ultrathin thickness by current synthetic protocols. Herein, a novel one-step wet-chemical method first proposed for protein-directed synthesis MnO
Many fundamental cell processes, such as angiogenesis, neurogenesis and cancer metastasis, are thought to be modulated by extracellular matrix stiffness. Thus, the availability of substrates having well-defined stiffness profiles can great importance in biophysical studies cell-substrate interaction. Here, we present a method fabricate biocompatible hydrogels with well defined linear gradient. This method, involving photopolymerization films progressively uncovering an...
Chirality can be used as a design tool to control the mechanical rigidity of hydrogels formed from self-assembling peptides. Hydrogels prepared enantiomeric mixtures β-hairpins show nonadditive, synergistic, enhancement in material compared gels either pure enantiomer, with racemic hydrogel showing greatest effect. CD spectroscopy, TEM, and AFM indicate that this is defined by nanoscale interactions between enantiomers self-assembled state.
It is still in high demand to develop extremely sensitive and accurate clinical tools for biomarkers of interest early diagnosis monitoring diseases. In this report, we present a highly compatible gold nanoparticle (AuNP)-based fluorescence-activatable probe sensing ultralow levels prostate-specific antigen (PSA) patient serum samples. The limit detection the newly developed PSA was pushed down 0.032 pg/mL, which more than 2 orders magnitude lower that conventional fluorescence probe....
The Plasmodium falciparum circumsporozoite protein (CSP) is critical for sporozoite function and invasion of hepatocytes. Given its nature, a phase III human CSP malaria vaccine trial ongoing. composed three regions as follows: an N terminus that binds heparin sulfate proteoglycans, four amino acid repeat region (NANP), C contains thrombospondin-like type I (TSR) domain. Despite the importance CSP, little known about structure. Therefore, recombinant forms were produced by expression in both...
Abstract Vesicle fusion is executed via formation of an Ω-shaped structure (Ω-profile), followed by closure (kiss-and-run) or merging the Ω-profile into plasma membrane (full fusion). Although limits release but recycles vesicles economically, facilitates couples to classical endocytosis for recycling. Despite its crucial role in determining exocytosis/endocytosis modes, how mediated poorly understood endocrine cells and neurons containing small ∼30–300 nm vesicles. Here, using confocal...
Seeing is believing: A rapid diagnostic platform for pathogen detection based on the acetylcholinesterase-catalyzed hydrolysis reaction has been developed. Owing to signal amplification strategies, sensitivity of this assay comparable that PCR. In addition, readout solution color change, which can be easily observed by naked eye alone. As a service our authors and readers, journal provides supporting information supplied authors. Such materials are peer reviewed may re-organized online...
Development of nontoxic, tumor-targetable, and potent in vivo RNA delivery systems remains an arduous challenge for clinical application RNAi therapeutics. Herein, we report a versatile nanoplatform based on tumor-targeted pH-responsive nanoformulas (NFs). The NF was engineered by combination artificial receptor, Zn(II)-DPA, with tumor-targetable drug-loadable hyaluronic acid nanoparticle, which further modified calcium phosphate (CaP) coating situ mineralization. can encapsulate...
A nanoscale RGD–pyrene–graphene oxide (GO) biosensor was prepared for real-time in situ detection of a cancer cell surface marker, integrin αvβ3. This GO-based is simple, robust, sensitive and high selectivity. It can also be adapted to other marker evaluation systems.
Phototherapies such as photodynamic therapy (PDT) and photothermal (PTT), due to their specific spatiotemporal selectivity minimal invasiveness, have been widely investigated alternative treatments of malignant diseases. Graphene its derivatives not only used carriers deliver photosensitizers for PDT, but also conversion agents (PTCAs) PTT. Herein, we strategically designed produced a novel photo-theranostic platform based on sinoporphyrin sodium (DVDMS) photosensitizer-loaded PEGylated...
Conventional evaluation methods of chemotherapeutic efficacy such as tissue biopsy and anatomical measurement are either invasive with potential complications or dilatory to capture the rapid pathological changes. Here, a sensitive resolution-scalable photoacoustic microscopy (PAM) theranostic nanoformulation was developed noninvasively monitor therapy response in timely manner. Ultrasmall graphene oxide nanosheets were designed both drug-loading vehicle signal amplifier tumor. With...
We developed a clinically translatable photothermal theranostic agent based on heptamethine dye, CySCOOH conjugated HSA, for multimodality imaging and tumor ablation.
Herein we report the design and synthesis of multifunctional VEGF-loaded IR800-conjugated graphene oxide (GO-IR800-VEGF) for multi-modality imaging-monitored therapeutic angiogenesis ischemic muscle. The as-prepared GO-IR800-VEGF positively targets VEGF receptors, maintains an elevated level in tissues a prolonged time, finally leads to remarkable Although more efforts are required further understand vivo behaviors long-term toxicology GO, our work demonstrates success using GO efficient...