A5084472936- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Electrospun Nanofibers in Biomedical Applications
- Photodynamic Therapy Research Studies
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- Porphyrin and Phthalocyanine Chemistry
- Lipid Membrane Structure and Behavior
- Tissue Engineering and Regenerative Medicine
- Hydrogels: synthesis, properties, applications
- biodegradable polymer synthesis and properties
- Connective tissue disorders research
- Tendon Structure and Treatment
- Corneal surgery and disorders
- Polymer Surface Interaction Studies
- Osteoarthritis Treatment and Mechanisms
- Silk-based biomaterials and applications
- Bone Tissue Engineering Materials
- Malaria Research and Control
- HIV Research and Treatment
- Mesenchymal stem cell research
- Collagen: Extraction and Characterization
- Developmental Biology and Gene Regulation
- Cellular transport and secretion
- Advanced Polymer Synthesis and Characterization
Federal Medical-Biological Agency
2018-2025
Sechenov University
2017-2025
Southwest Washington Medical Center
2025
Oregon Health & Science University
2023-2025
Semenov Institute of Chemical Physics
2014-2023
Federal Scientific Research Centre Crystallography and Photonics
2018-2020
California Institute for Regenerative Medicine
2018-2020
Russian Academy of Sciences
2012-2019
State Research Institute of Highly Pure Biopreparations
2018
Opinion Leader Research
2018
The Plasmodium falciparum circumsporozoite protein (CSP) is critical for sporozoite function and invasion of hepatocytes. Given its nature, a phase III human CSP malaria vaccine trial ongoing. composed three regions as follows: an N terminus that binds heparin sulfate proteoglycans, four amino acid repeat region (NANP), C contains thrombospondin-like type I (TSR) domain. Despite the importance CSP, little known about structure. Therefore, recombinant forms were produced by expression in both...
Abstract Previous phase I studies demonstrated safety and some beneficial effects of mesenchymal stem cells (MSCs) in patients with mild to moderate idiopathic pulmonary fibrosis (IPF). The aim our study was evaluate the safety, tolerability, efficacy a high cumulative dose bone marrow MSCs rapid progressive course severe IPF. Twenty forced ventilation capacity (FVC) ≥40% diffusing lung for carbon monoxide (DLCO) ≥20% decline both >10% over previous 12 months were randomized into two...
Development of fast force volume (FFV), PeakForce Tapping (PFT), and related AFM techniques allow acquisition mapping a sample's mechanical properties. The methods are well-suited for studying soft biological samples like living cells in liquid environment. However, the question remains how measured properties to those acquired with classical (FV) technique conducted at low indentation rates. difference is coming mostly from pronounced viscoelastic behavior cells, making apparent elastic...
We have designed a novel two-component matrix (SPRPix) for the encapsulation of directly reprogrammed human neural precursor cells (drNPC). The is comprised 1) solid anisotropic complex scaffold prepared by electrospinning mixture recombinant analogues spider dragline silk proteins - spidroin 1 (rS1/9) and 2 (rS2/12) polycaprolactone (PCL) (rSS-PCL), 2) "liquid matrix" based on platelet-rich plasma (PRP). combination PRP promoted drNPC proliferation with formation tissue organoids...
Growth plate and articular cartilage constitute a single anatomical entity early in development but later separate into two distinct structures by the secondary ossification center (SOC). The reason for such separation remains unknown. We found that evolutionarily SOC appears animals conquering land - amniotes. Analysis of pattern mammals with specialized extremities (whales, bats, jerboa) revealed correlates extent mechanical loads. Mathematical modeling reduces stress within growth plate....
Fibrin is a well-known tool in tissue engineering, but the structure of its modifications created to improve properties remains undiscussed despite their importance,<italic>e.g.</italic>in designing biomaterials that ensure cell migration and lumenogenesis.
The interaction of neural progenitor cells (NPCs) with the extracellular matrix (ECM) plays an important role in tissue regeneration. Understanding which motifs ECM proteins are crucial for normal NPC adhesion, proliferation, and differentiation is order to create more adequate engineered models efficiently study central nervous system regeneration mechanisms. We have shown earlier that anisotropic matrices prepared from a mixture recombinant dragline silk proteins, such as spidroin 1 2, by...
A series of well-defined poly(d,l-lactide) and poly(ε-caprolactone) macromonomers (Mn ∼ 600 ∼1200 Da) bearing a (meth)acrylate group at one side the chain either hydrophobic nonpolar butyl or hydrophilic polar hydroxyl other were synthesized via 1,8-diazabicyclo[5.4.0]undec-7-ene-catalyzed ring-opening polymerization (ROP) d,l-lactide methanesulfonic acid-catalyzed ROP ε-caprolactone, respectively. Then, random copolymers 30 000 Da, Đ < 1.25) prepared reversible addition–fragmentation...
The aim was to conduct a comparative morphological analysis of the effect implantation and modified adenovirus Ad 5/3 on efficiency local gene delivery cells peri-implantation niche in vivo. Material methods . experiments were carried out laboratory rats, which implanted with collagen membranes for period 2 weeks. Three seven days after introduction adenoviruses (wt, 5/3), tissues fixed histological examination transduced by situ PCR. Results. results showed that presence implant improved...
Abstract The morphological characterisation is crucial for analysing cell states, especially red blood cells (RBCs), which are used in transfusions. This study compared the applicability of atomic force microscopy (AFM) and confocal optical profilometry accurate RBC parameters. imaging RBCs thawed after cryopreservation with immediate delayed washing steps (deglycerolisation) was performed, data obtained AFM were clinical laboratory studies. Both techniques provided close on parameters, but...
The mechanical properties of soft gels hold significant relevance in biomedicine and biomaterial design, including the development tissue engineering constructs bioequivalents. It is important to adequately characterize gel's since they play a role both overall structural construct physiological responses cells. question remains which approach for characterization most suitable specific biomaterials. Our investigation centered on comparison three types four distinct testing techniques: shear...
Thrombospondin repeat (TSR)-like domains are structures involved with cell adhesion. Plasmodium falciparum proteins containing TSR play crucial roles in parasite development. In particular, the preerythrocytic P. circumsporozoite protein is hepatocyte invasion. The importance of these two other malaria proteins, merozoite-specific thrombospondin-related anonymous (MTRAP) and apical membrane (PTRAMP), were assessed using near-full-length recombinant composed extracellular produced Escherichia...
Abstract Decellularized bovine pericardium (DBP)‐based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after transplantation, various chemical cross‐linking strategies employed. However, structural and functional properties of differ dependence on cross‐linker used. Here, we performed a bottom‐up study alterations DBP‐based following with hexamethylene diisocyanate (HMDC), ethylene glycol diglycidyl ether (EGDE),...
Abstract Instrumented indentation has become an indispensable tool for quantitative analysis of the mechanical properties soft polymers and biological samples at different length scales. These types are known their prominent viscoelastic behavior, attempts to calculate such from data constantly made. The simplest experiment presents a cycle approach (deepening into sample) retraction indenter, with output force depth as functions time versus dependency (force curve). linear theory based on...
Abstract There is a growing demand on the studies of wound healing potentials photodynamic therapy. Here we analyze effects Fotoditazin, an e6 chlorine derivative, and its complexes with amphiphilic polymers, early stage in rat model. A skin excision model prevented contraction was developed male albino rats divided into eight groups according to treatment mode. All animals received injections one studied compositions their beds underwent low‐intensity laser irradiation or stayed...
Abstract Background The nucleus, besides its functions in the gene maintenance and regulation, plays a significant role cell mechanosensitivity mechanotransduction. It is largest cellular organelle that often considered as stiffest part well. Interestingly, previous studies have revealed nucleus might be dispensable for some of properties, like polarization 1D 2D migration. Here, we studied how nanomechanical properties cells, measured using mapping by atomic force microscopy (AFM), were...