A5072014139- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Additive Manufacturing and 3D Printing Technologies
- Computational Drug Discovery Methods
- Tissue Engineering and Regenerative Medicine
- Polymer Surface Interaction Studies
- biodegradable polymer synthesis and properties
- Hydrogels: synthesis, properties, applications
- Collagen: Extraction and Characterization
- Analytical Chemistry and Sensors
- Polymer composites and self-healing
- Corneal surgery and disorders
- Electrochemical Analysis and Applications
- Elasticity and Material Modeling
- Nanofabrication and Lithography Techniques
- Blood properties and coagulation
- Microfluidic and Bio-sensing Technologies
- thermodynamics and calorimetric analyses
- Supramolecular Self-Assembly in Materials
- Photopolymerization techniques and applications
- Neuroscience and Neural Engineering
- Lipid Membrane Structure and Behavior
Sechenov University
2019-2025
Purdue University West Lafayette
2017-2024
Lomonosov Moscow State University
2011-2022
National University of Science and Technology
2020
Institute of Nanotechnology
2019
Moscow State University
2011-2012
Force-displacement (F-Z) curves are the most commonly used Atomic Force Microscopy (AFM) mode to measure local, nanoscale elastic properties of soft materials like living cells. Yet a theoretical framework has been lacking that allows post-processing F-Z data extract their viscoelastic constitutive parameters. Here, we propose new method samples cells and hydrogels directly from conventional AFM experiments, thereby creating common platform for analysis cell with arbitrary linear relations....
Glioblastoma (GBM) is an intractable malignancy with high recurrence and mortality. Combinatorial therapy based on temozolomide (TMZ) cisplatin (CDDP) shows promising potential for GBM in clinical trials. However, significant challenges include limited blood-brain-barrier (BBB) penetration, poor targeting of tissue/cells, systemic side effects, which hinder its efficacy therapy. To surmount these challenges, new GBM-cell membrane camouflaged pH-sensitive biomimetic nanoparticles (MNPs)...
Biological self-assembly is crucial in the processes of development, tissue regeneration, and maturation bioprinted tissue-engineered constructions. The cell aggregates-spheroids-have become widely used model objects study this phenomenon. Existing approaches describe fusion aggregates by analogy with coalescence liquid droplets ignore complex structural properties spheroids. Here, we analyzed process connection structure mechanical spheroids from human somatic cells different phenotypes:...
Development of fast force volume (FFV), PeakForce Tapping (PFT), and related AFM techniques allow acquisition mapping a sample's mechanical properties. The methods are well-suited for studying soft biological samples like living cells in liquid environment. However, the question remains how measured properties to those acquired with classical (FV) technique conducted at low indentation rates. difference is coming mostly from pronounced viscoelastic behavior cells, making apparent elastic...
Abstract The measurement of local mechanical properties living cells by nano/micro indentation relies on the foundational assumption locally isotropic cellular deformation. As a consequence assumed isotropy, cell membrane and underlying cytoskeleton are expected to deform axisymmetrically when indented spherical tip. Here, we directly observe geometry deformation different adherent during nanoindentation with integrated Atomic Force (AFM) spinning disk confocal (SDC) microscope. We show that...
Mechanical properties of living cells determined by cytoskeletal elements play a crucial role in wide range biological functions.
The improvement of biomedical properties, e.g. biocompatibility, poly(3-hydroxyalkanoates) (PHAs) by copolymerization is a promising trend in bioengineering. We used strain Azotobacter chroococcum 7B, an effective producer PHAs, for biosynthesis not only poly(3-hydroxybutyrate) (PHB) and its main copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV), but also alternative poly(3-hydroxybutyrate)-poly(ethylene glycol) (PHB-PEG). In we sucrose as the primary carbon source valeric...
Abstract Recent developments such as multi-harmonic Atomic Force Microscopy (AFM) techniques have enabled fast, quantitative mapping of nanomechanical properties living cells. Due to their high spatiotemporal resolution, these methods provide new insights into changes mechanical subcellular structures due disease or drug response. Here, we propose three improvements significantly improve the identification, and property quantification sub-cellular sub-nuclear using AFM on First,...
The viscoelastic parameters of the cell can report on state, cellular processes and diseases. Cell mechanics strongly rely properties cytoskeleton, an important system subcellular filaments, especially high-level structures that actin forms together with actin-binding proteins (ABPs). In normal cells, components cytoskeleton are highly integrated, their functions well orchestrated. contrast, impaired expression functioning ABPs lead to increasing ability cancer cells resist chemotherapy...
Cell viability is the primary integrative parameter used for various purposes, particularly when fabricating tissue equivalents (e.g., using bioprinting or scaffolding techniques), optimizing conditions to cultivate cells, testing chemicals, drugs, and biomaterials, etc. Most of conventional methods were originally designed a monolayer (2D) culture; however, 2D approaches fail adequately assess tissue-engineered construct's drug effects recapitulate host-pathogen interactions infectivity....
Abstract The self‐assembly of peptides is a key direction for fabrication advanced materials. Novel approaches fine tuning macroscopic and microscopic properties peptide self‐assemblies are high demand constructing biomaterials with desired properties. In this work, while studying the kinetics Fmoc‐Diphenylalanine (Fmoc‐FF) dipeptide using Thioflavin T (ThT) dye, we observed that presence ThT strongly modifies structural mechanical Fmoc‐FF hydrogel. Notably, resulted in tenfold increase...
Macrophages are the major players and orchestrators of inflammatory response. Expressed proteins secreted cytokines have been well studied for two polar macrophage phenotypes-pro-inflammatory M1 anti-inflammatory regenerative M2, but little is known about how polarization modulates functions. In this study, we used biochemical biophysical methods to compare functional activity mechanical properties activated human macrophages differentiated from monocyte with GM-CSF (M0_GM) M-CSF (M0_M)...