A5001638982- biodegradable polymer synthesis and properties
- Electrospun Nanofibers in Biomedical Applications
- Microplastics and Plastic Pollution
- Advanced Polymer Synthesis and Characterization
- Carbon dioxide utilization in catalysis
- Bone Tissue Engineering Materials
- Graphene and Nanomaterials Applications
- Legume Nitrogen Fixing Symbiosis
- 3D Printing in Biomedical Research
- Biopolymer Synthesis and Applications
- Microbial Metabolic Engineering and Bioproduction
- Additive Manufacturing and 3D Printing Technologies
- Hydrogels: synthesis, properties, applications
- Dental Implant Techniques and Outcomes
- Orthopaedic implants and arthroplasty
- Surgical Sutures and Adhesives
- Advanced Sensor and Energy Harvesting Materials
- Enzyme Catalysis and Immobilization
- Nanoparticle-Based Drug Delivery
- Metabolism and Genetic Disorders
- Probiotics and Fermented Foods
- Mesenchymal stem cell research
- Agricultural Productivity and Crop Improvement
- Microbial Community Ecology and Physiology
- Seaweed-derived Bioactive Compounds
Federal Center Research Fundamentals of Biotechnology
2016-2025
Russian Academy of Sciences
2013-2024
A N Bach Institute of Biochemistry
2012-2023
Biotechnology Research Center
2016-2022
Lomonosov Moscow State University
2005-2015
Semenov Institute of Chemical Physics
2005
Biodegradable and biocompatible polymers, polyhydroxyalkanoates (PHAs), are actively used in medicine to produce a wide range of medical devices dosage formulations. The industry mainly utilizes PHAs obtained by chemical synthesis, but interest the application natural biotechnologically is also growing. Synthetic biomimetic analogs bacterial poly(3-hydroxybutyrate) (PHB) other PHAs. This paper addresses issue presence biological activity synthetic (stimulation cell proliferation...
Peripheral nerve injury poses a threat to the mobility and sensitivity of nerve, thereby leading permanent function loss due low regenerative capacity mature neurons. To date, most widely clinically applied approach bridging injuries is autologous grafting, which faces challenges such as donor site morbidity, shortages, necessity second surgery. An effective therapeutic strategy urgently needed worldwide overcome current limitations. Herein, magnetic guidance conduit (NGC) based on...
Hydrolytic degradations of biodegradable poly(3-hydroxybutyrate) (PHB), polylactide (PLA) and their derivatives were explored by kinetic structure methods at 37 70°C in phosphate buffer. It was revealed the profiles for copolymer PHBV (20% 3-hydroxyvalerate) blend PHB-PLA (1:1 wt. ratio). The intensity biopolymer hydrolysis depending on temperature is characterized total weight loss viscosity-averaged molecular decrement (ΔMW) as well WAXS AMF techniques. Characterization PHB includes both...
The improvement of biomedical properties, e.g. biocompatibility, poly(3-hydroxyalkanoates) (PHAs) by copolymerization is a promising trend in bioengineering. We used strain Azotobacter chroococcum 7B, an effective producer PHAs, for biosynthesis not only poly(3-hydroxybutyrate) (PHB) and its main copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV), but also alternative poly(3-hydroxybutyrate)-poly(ethylene glycol) (PHB-PEG). In we sucrose as the primary carbon source valeric...
The hydrolytic and enzymatic degradation of polymer films poly(3-hydroxybutyrate) (PHB) different molecular mass its copolymers with 3-hydroxyvalerate (PHBV) (3-HV) content mass, 3-hydroxy-4-methylvalerate (PHB4MV), polyethylene glycol (PHBV-PEG) produced by the Azotobacter chroococcum 7B controlled biosynthesis technique were studied under in vitro model conditions. changes physicochemical properties polymers during their pancreatic lipase solution phosphate-buffered saline for a long time...
Over the past century there was a significant development and extensive application of biodegradable biocompatible polymers for their biomedical applications. This research investigates dynamic change in properties polymers: poly(3-hydroxybutyrate (PHB), poly-l-lactide (PLA), 50:50 blend (PHB/PLA)) during hydrolytic non-enzymatic (in phosphate buffered saline (PBS), at pH = 7.4, 37 °C) enzymatic degradation PBS supplemented with 0.25 mg/mL pancreatic lipase). 3T3 fibroblast proliferation on...
The copolymerization of poly(3-hydroxybutyrate) (PHB) is a promising trend in bioengineering to improve biomedical properties, e.g. biocompatibility, this biodegradable polymer. We used strain Azotobacter chroococcum 7B, an effective producer PHB, for biosynthesis not only homopolymer and its main copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV), but also novel terpolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-poly(ethylene glycol) (PHB-HV-PEG), using sucrose as the...
Magnetically responsive composite polymer scaffolds have good potential for a variety of biomedical applications. In this work, electrospun made polyhydroxybutyrate (PHB) and magnetite (Fe3O4) particles (MPs) were studied before after degradation in either PBS or lipase solution. MPs different sizes with high saturation magnetization synthesized by the coprecipitation method followed coating citric acid (CA). Nanosized prone to magnetite–maghemite phase transformation during scaffold...
Millions of people worldwide suffer from musculoskeletal damage, thus using the largest proportion rehabilitation services. The limited self-regenerative capacity bone and cartilage tissues necessitates development functional biomaterials. Magnetoactive materials are a promising solution due to clinical safety deep tissue penetration magnetic fields (MFs) without attenuation heating. Herein, electrospun microfibrous scaffolds were developed based on piezoelectric poly(3-hydroxybutyrate)...
IntroductionFor the last three decades intensive research studies and developments of polymer systems for controlled release pharmaceuticals have been carried out [1][2][3].At curative dose prolonged delivery drugs from into organism permits to eliminate shortcomings in peroral, injectable, aerosol, other traditional methods drug administration.Among those hypertoxicity, instability, pulsative character rate delivery, ineffective expenditure should be pointed out.Alternatively, applications...
This review is designed to be a comprehensive source for biodegradable polymer: poly(3-hydroxybutyrate)—PHB research, including fundamental structure/property relationships and biodegradation kinetics samples of different geometry. In addition presenting the scientific framework advances in PHB this focuses on applications biomedicine environment with discussion commercial health/safety concerns materials.
This study investigated the effect of various cultivation conditions (sucrose/phosphate concentrations, aeration level) on alginate biosynthesis using bacterial producing strain Azotobacter vinelandii 12 by full factorial design (FFD) method and physicochemical properties (e.g., rheological properties) produced alginate. We demonstrated experimentally applicability for tissue engineering (the cytotoxicity testing mesenchymal stem cells (MSCs)). The isolated synthesis high molecular weight...
Abstract This review is designed to be a comprehensive source for biodegradable polymer: poly(3-hydroxybutyrate) research, including fundamental structure/property relationships and biodegradation kinetics samples of different geometry. In addition presenting the scientific framework advances in PHB this focuses on applications biomedicine environment with discussion commercial health/safety concerns materials. Keywords: Applicationsbiocompatibilitybiodegradation soildegradation animal...
A study of in vitro biodegradation poly(3-hydroxybutyrate) (PHB) model conditions was performed. The porcine pancreatic lipase solutions at different concentrations the two buffer systems (phosphate-buffered saline (PBS) and simulating body fluid (SBF)) were chosen as media. At first, optimal concentration (0.25 mg/ml PBS) determined: these media decomposition PHB films realized faster according to data gravimetric analysis. Differential scanning calorimetry showed an increase crystallinity...
A precursor feeding strategy for effective biopolymer producer strain Azotobacter chroococcum 7B was used to synthesize various poly(3-hydroxybutyrate) (PHB) copolymers. We performed experiments on biosynthesis of PHB copolymers by A. using precursors: sucrose as the primary carbon source, carboxylic acids and ethylene glycol (EG) derivatives [diethylene (DEG), triethylene (TEG), poly(ethylene glycol) (PEG) 300, PEG 400, 1000] additional sources. analyzed growth parameters including biomass...