A5019569012- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Force Microscopy Techniques and Applications
- Neuroscience and Neural Engineering
- Conducting polymers and applications
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Electrocatalysts for Energy Conversion
- Advancements in Battery Materials
- Lipid Membrane Structure and Behavior
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Nanopore and Nanochannel Transport Studies
- Electron and X-Ray Spectroscopy Techniques
- Advanced Battery Technologies Research
- Graphene research and applications
- Microfluidic and Capillary Electrophoresis Applications
- 2D Materials and Applications
- Fuel Cells and Related Materials
- Advanced Photocatalysis Techniques
- Anodic Oxide Films and Nanostructures
- Advanced battery technologies research
- Diamond and Carbon-based Materials Research
- Molecular Junctions and Nanostructures
- Advanced Chemical Sensor Technologies
Life Science Institute
2018-2025
Nagoya University
2022-2025
Kanazawa University
2016-2025
Graduate School USA
2017-2025
Japan Science and Technology Agency
2015-2024
Tohoku University
2009-2018
Advanced Institute of Materials Science
2012-2018
Japan Foundation London
2013
Institute of Materials Research of the Slovak Academy of Sciences
2013
Imperial College London
2010-2012
We described a hybrid system of scanning electrochemical microscopy (SECM) and ion conductance (SICM) with current feedback nanopositioning control for simultaneous imaging noncontact topography spatial distribution species. A nanopipette/nanoring electrode probe provided submicrometer resolution the measurement on surfaces complex topology. The SECM/SICM had an aperture radius 220 nm. inner outer radii SECM Au nanoring were 330 550 nm, respectively. Characterization was performed electron...
Double take: Double-barrel carbon nanoprobes with integrated distance control for simultaneous nanoscale electrochemical and ion conductance microscopy can be fabricated a wide range of probe sizes in less than two minutes. The allow noncontact topographical (left image) imaging (right) living neurons, as well localized K+ delivery neurotransmitter detection. Detailed facts importance to specialist readers are published "Supporting Information". Such documents peer-reviewed, but not...
The measurement of key molecules in individual cells with minimal disruption to the biological milieu is next frontier single-cell analyses. Nanoscale devices are ideal analytical tools because their small size and potential for high spatial temporal resolution recordings. Here, we report fabrication disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within range 5-200 nm. functionalization nanoelectrode platinum allowed monitoring oxygen consumption outside inside a brain...
Abstract High‐resolution scanning electrochemical cell microscopy (SECCM) is used to image and quantitatively analyze the hydrogen evolution reaction (HER) catalytically active sites of 1H‐MoS 2 nanosheets, MoS , WS heteronanosheets. Using a 20 nm radius nanopipette hopping mode scanning, resolution SECCM was beyond optical limit visualized small triangular nanosheet with side length ca. 130 nm. The provides local cyclic voltammograms nanoscale spatial for visualizing HER as images. activity...
Electrochemical CO2 reduction is a key technology to recycle as renewable resource, but adsorbing on the catalyst surface challenging. We explored effects of reduced graphene oxide (rGO) in Sn/rGO composites and found that adsorption ability was almost 4-times higher than bare Sn catalysts. Density functional theory calculations revealed oxidized groups rGO offered sites for toward adjacent CO2-rich conditions near facilitated production formate via COOH* formation while suppressing CO*...
Tubular structures of transition metal dichalcogenides (TMDCs) have attracted attention in recent years due to their emergent physical properties, such as the giant bulk photovoltaic effect and chirality-dependent superconductivity. To understand control these it is highly desirable develop a sophisticated method fabricate TMDC tubular with smaller diameters more uniform crystalline orientation. For this purpose, rolling up monolayers into nanoscrolls an attractive approach fabricating...
We describe voltage-switching mode scanning electrochemical microscopy (VSM-SECM), in which a single SECM tip electrode was used to acquire high-quality topographical and images of living cells simultaneously. This achieved by switching the applied voltage so as change faradaic current from hindered diffusion feedback signal (for distance control imaging) flux measurement interest. imaging method is robust, nanoscale electrode, simple produce, for both topography activity measurements. In...
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTScanning Probe Microscopy for Nanoscale Electrochemical ImagingYasufumi Takahashi†‡, Akichika Kumatani§∥, Hitoshi Shiku⊥, and Tomokazu Matsue*§∥View Author Information† Division of Electrical Engineering Computer Science, Kanazawa University, 920-1192, Japan‡ Precursory Research Embryonic Science Technology (PRESTO), Japan Agency (JST), Saitama 332-0012, Japan§ Advanced Institute Materials Research, Tohoku Sendai 980-8577, Japan∥ Graduate School...
Carbon-based metal-free catalysts for the hydrogen evolution reaction (HER) are essential development of a sustainable society. Identification active sites in heterogeneous catalysis is key rational design low-cost and efficient catalysts. Here, by fabricating holey graphene with chemically dopants, atomic-level mechanism accelerating HER chemical dopants unveiled, through elemental mapping atomistic characterizations, scanning electrochemical cell microscopy (SECCM), density functional...
Nanometric field-effect-transistor (FET) sensors are made on the tip of spear-shaped dual carbon nanoelectrodes derived from deposition inside double-barrel nanopipettes. The easy fabrication route allows semiconductors or conducting polymers to comprise transistor channel. A channel electrodeposited poly pyrrole (PPy) exhibits high sensitivity toward pH changes. This property is exploited by immobilizing hexokinase PPy nano-FETs give rise a selective ATP biosensor. Extracellular and...
Abstract Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding role H + in cellular and subcellular processes, with particular importance cancer. While several pHe sensing techniques have been developed, accessing this information requires improvement sensitivity, spatial temporal resolution. We report on a zwitterionic label-free nanoprobe that addresses these long-standing challenges. The probe has sensitivity > 0.01 units, 2 ms response time,...
In vivo monitoring of reactive oxygen species (ROS) in tumors during treatment with anticancer therapy is important for understanding the mechanism action and design new drugs. this work, a platinized nanoelectrode placed into single cell detection ROS signal, drug-induced production then recorded. The main advantages method are short incubation time drug its high sensitivity which allows low intracellular concentrations. We have shown that our can measure response to chemotherapy...
To visualize the electrochemical reactivity and obtain diffusion coefficient of anode lithium-ion batteries, we developed scanning cell microscopy (SECCM) in a glovebox.
Transition metal dichalcogenides (TMDs) exhibit unique properties and potential applications when reduced to one-dimensional (1D) nanoribbons (NRs), owing quantum confinement high edge densities. However, effective growth methods for self-aligned TMD NRs are still lacking. We demonstrate a versatile approach lattice-guided of dense, aligned MoS 2 NR arrays via chemical vapor deposition (CVD) on anisotropic sapphire substrates, without tailored surface steps. This method enables the synthesis...
Because endogenous extracellular vesicles are involved in important physiological functions, various techniques have been developed for their isolation and evaluation. However, methods evaluating within cells limited. This study presents a technique the direct extraction evaluation of intraluminal (ILVs). combines scanning ion conductance microscopy, electrochemical syringes, confocal microscopy to extract specific structures living cell, achieving high spatial resolution accuracy at...
Scanning ion conductance microscopy (SICM) using a nanopipette as probe and ionic current feedback signal was introduced novel technique to study live cells in physiological environment. To avoid contact between the pipette tip during conventional lateral scanning mode, we adopted standing approach (STA) mode which moved vertically first then retracted from cell surface at each measurement point on an XY plane. The STA ensured non-contact imaging of topography for wide range uneven...
A membrane protein on the surface of a single living mammalian cell was imaged by scanning electrochemical microscopy (SECM). The epidermal growth factor receptor (EGFR) is one key proteins associated with cancer. It elicits wide range cell-type-specific responses, leading to proliferation, differentiation, apoptosis, and migration. To estimate EGFR expression levels SECM, labeled alkaline phosphatase (ALP) via an antibody. oxidation current PAP (p-aminophenol) produced ALP-catalyzed...
We propose a novel electrochemical detection system for alkaline phosphatase (ALP) activity using the difference in water and oil solubilities between substrate, ferrocene ethyl phosphate ester (FcEtOPO32–), enzymatic product, ferroceneethanol (FcEtOH). In this system, droplets containing ALP FcEtOPO32– were placed on Pt disk microelectrode surrounded by mineral oil. By ALP-catalyzed reaction, was converted to FcEtOH, which then transferred from with remaining droplets. After partitioning...
In this study, we developed a novel method for fabricating microwell arrays constructed from alginate gels, and the gel microwells were used three-dimensional (3D) cell culture. The fabricated on patterned ITO electrode using electrodeposition. Embryonic stem (ES) cells or hepatocellular carcinoma (HepG2) cultured in containing 3T3 cells. During culture, embryoid bodies (EBs) HepG2 spheroids successfully microwells. oxygen consumption of EBs indicated that they cultured. Liver-specific gene...
Using nanopipettes to locally deliver molecules the surface of living cells could potentially open up studies biological processes down level single molecules. However, in order achieve precise and quantitative local delivery it is essential be able determine amount distribution being delivered. In this work, we investigate how size nanopipette, magnitude applied pressure or voltage, which drives delivery, distance underlying influences number spatial delivered Analytical expressions...
Observation of nanoscale structure dynamics on cell surfaces is essential to understanding functions. Hopping-mode scanning ion conductance microscopy (SICM) was used visualize the topography fragile convoluted structures under noninvasive conditions. However, conventional hopping mode SICM does not have sufficient temporal resolution observe cell-surface in situ because additional time required for performing vertical probe movements nanopipette. Here, we introduce a new algorithm high...
Abstract Despite extensive use of arginine‐rich cell‐penetrating peptides (CPPs)—including octaarginine (R8)—as intracellular delivery vectors, mechanisms for their internalization are still under debate. Lipid packing in live cell membranes was characterized using a polarity‐sensitive dye (di‐4‐ANEPPDHQ), and evaluated terms generalized polarization. Treatment with membrane curvature‐inducing led to significant loosening the lipid packing, resulting an enhanced R8 penetration....
In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 electrodes with pitch 250 μm for rapid electrochemical imaging large areas. spheroids were stimulated K+ dopamine. Poststimulation was electrochemically imaged using device. clearly showed effects dopaminergic drugs l-3,4-dihydroxyphenylalanine...
Information regarding spatial mRNA localization in single cells is necessary for a better understanding of cellular functions tissues. Here, we report method evaluating using double-barrel scanning ion conductance microscopy (SICM). Two barrels nanopipette were filled with aqueous and organic electrolyte solutions used SICM as an electrochemical syringe, respectively. We confirmed that the phase barrel could be to collect cytosol from living cells, which minute but sufficient amount assess...