A5006294489- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Single-cell and spatial transcriptomics
- Neural dynamics and brain function
- Cell Image Analysis Techniques
- Gene Regulatory Network Analysis
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Neuroendocrine regulation and behavior
- Ubiquitin and proteasome pathways
- Advanced Electron Microscopy Techniques and Applications
- Functional Brain Connectivity Studies
- Receptor Mechanisms and Signaling
- Peptidase Inhibition and Analysis
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- RNA Research and Splicing
- Congenital heart defects research
- Genomics and Rare Diseases
- Intestinal and Peritoneal Adhesions
- RNA regulation and disease
- Advanced Steganography and Watermarking Techniques
- Escherichia coli research studies
- Metastasis and carcinoma case studies
- Neuroscience, Education and Cognitive Function
University of Utah
2023-2024
University of California, San Francisco
2013-2021
University of Maryland, Baltimore
2009-2015
Czech Academy of Sciences, Institute of Physiology
2014
Princeton University
2014
Cereal Disease Laboratory
2006
National Institutes of Health
2006
Calcium/calmodulin-dependent protein kinase II (CaMKII) is essential for synaptic plasticity underlying memory formation. Some functions of CaMKII are mediated by interactions with proteins, and activity-triggered translocation to synapses has been heavily studied. However, actions away from the postsynaptic density (PSD) remain poorly understood, in part because difficulty discerning where binds live cells. We used photoactivated localization microscopy (PALM) rat hippocampal neurons track...
The family of Shank scaffolding molecules (comprising Shank1, 2 and 3) are core components the postsynaptic density (PSD) in neuronal synapses. Shanks link surface receptors to other within PSD, as well actin cytoskeleton. However, determining function proteins neurons has been complicated because different isoforms share a very high degree sequence domain homology. Therefore, control content while minimizing potential compensatory effects, miRNA-based knockdown strategy was developed reduce...
Abstract To examine the consequences of inhibiting activator protein-1 (AP-1) transcription factors in skin, transgenic mice were generated, which use tetracycline system to conditionally express A-FOS, a dominant negative that inhibits AP-1 DNA binding. Older develop mild alopecia and hyperplasia sebaceous glands, particularly around eyes. When A-FOS was expressed during chemical-induced skin carcinogenesis, do not characteristic benign malignant squamous lesions but instead adenomas...
A temporal phase mask encryption method is proposed and experimentally demonstrated to improve the security of stealth channel in an optical steganography system. The protected two levels. In first level, data carried by amplified spontaneous emission (ASE) noise, which cannot be detected either time domain or spectral domain. second even if eavesdropper suspects existence channel, each bit covered a fast changing mask. code always combined with wide band noise from ASE. Without knowing...
New technologies make it possible to measure activity from many neurons simultaneously. One approach is analyze simultaneously recorded individually, then group together which increase their during similar behaviors into an “ensemble.” However, this notion of ensemble ignores the ability act collectively and encode transmit information in ways that are not reflected by individual levels. We used microendoscopic GCaMP imaging prefrontal while mice were either alone or engaged social...
Prolonged social isolation induces a distinct state characterized by numerous impacts on behavior, including increased aggression and altered behaviors, but the contributions of cortical circuits to these behaviors are poorly understood. Here, we find that promotes increases investigatory leading up in both males female mice. Genetic characterization neuropeptidergic population Tachykinin-2 expressing (Tac2+) neurons medial prefrontal cortex (mPFC) reveals predominately early-layer GABAergic...
Type IV pili (T4P) are filamentous surface appendages required for tissue adherence, motility, aggregation, and transformation in a wide array of bacteria archaea. The bundle-forming pilus (BFP) enteropathogenic Escherichia coli (EPEC) is prototypical T4P confirmed virulence factor. fibers assembled by complex biogenesis machine that extrudes through an outer membrane (OM) pore formed the secretin protein. Secretins constitute superfamily proteins assemble into multimers support transport...
Renal sodium-dependent phosphate transporter 2a (Npt2a) binds to a number of PDZ adaptor proteins including sodium-hydrogen exchanger regulatory factor-1 (NHERF-1), which regulates its retention in the apical membrane renal proximal tubule cells and response parathyroid hormone (PTH). The present experiments were designed study lateral mobility enhanced green fluorescent protein (EGFP)-Npt2a tubule-like opossum kidney (OK) using fluorescence recovery after photobleaching (FRAP) determine...
In neurons, the shape of dendritic spines relates to synapse function, which is rapidly altered during experience-dependent neural plasticity. The small size makes detailed measurement their morphology in living cells best suited super-resolution imaging techniques. distribution molecular positions mapped via live-cell Photoactivated Localization Microscopy (PALM) a powerful approach, but motion complicates this analysis and can degrade overall resolution morphological reconstruction....
Social interactions are encoded by the coordinated activity of heterogeneous cell types within distributed brain regions including medial prefrontal cortex (mPFC). However, our understanding which comprise social ensemble has been limited available mouse lines and reliance on single marker genes. We identified differentially active neuronal populations during quantifying immediate-early gene (IEG) expression using snRNA-sequencing. These studies revealed that distinct neuron composed...
Abstract The prefrontal cortex plays a key role in social interactions, anxiety-related avoidance, and flexible context- dependent behaviors, raising the question: how do neurons represent socioemotional information across different environments? Are contextual representations segregated or intermixed, does this cause encoding to remap generalize To address this, we imaged neuronal activity medial of mice engaged interactions avoidance within environments. Neuronal ensembles representing...
ABSTRACT New technologies have made it possible to measure activity from many neurons simultaneously. Nevertheless, most studies still analyze the of simultaneously recorded one-at-a-time, then group together which increase their during similar behaviors into an ‘ensemble.’ This notion ensemble ignores ability act collectively, and encode transmit information in ways that are not reflected by individual levels. We used microendoscopic GCaMP imaging prefrontal while mice were either alone or...
Supplementary Methods and Materials from Activator Protein-1 Activity Regulates Epithelial Tumor Cell Identity
Supplementary Methods and Materials from Activator Protein-1 Activity Regulates Epithelial Tumor Cell Identity
<div>Abstract<p>To examine the consequences of inhibiting activator protein-1 (AP-1) transcription factors in skin, transgenic mice were generated, which use tetracycline system to conditionally express A-FOS, a dominant negative that inhibits AP-1 DNA binding. Older develop mild alopecia and hyperplasia sebaceous glands, particularly around eyes. When A-FOS was expressed during chemical-induced skin carcinogenesis, do not characteristic benign malignant squamous lesions but...
<div>Abstract<p>To examine the consequences of inhibiting activator protein-1 (AP-1) transcription factors in skin, transgenic mice were generated, which use tetracycline system to conditionally express A-FOS, a dominant negative that inhibits AP-1 DNA binding. Older develop mild alopecia and hyperplasia sebaceous glands, particularly around eyes. When A-FOS was expressed during chemical-induced skin carcinogenesis, do not characteristic benign malignant squamous lesions but...
<h3>Objective:</h3> This case presents a patient with cognitive decline and spasticity associated previously unidentified pathogenic variant of presinilin1 (PSEN1). <h3>Background:</h3> Mutations in PSEN1 are the most common cause familial Alzheimer's Disease (AD). highly penetrant autosomal dominant inheritance, presenting at young age often unusual features including spastic paraplegia (SP). We report novel mutation found our that is suspected to be his AD SP. <h3>Design/Methods:</h3>...