A5075093223- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Spaceflight effects on biology
- Reproductive Biology and Fertility
- Amyotrophic Lateral Sclerosis Research
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Neurogenetic and Muscular Disorders Research
- Neurological diseases and metabolism
- Photoreceptor and optogenetics research
- Nanoparticles: synthesis and applications
- Photosynthetic Processes and Mechanisms
- Cell death mechanisms and regulation
- Birth, Development, and Health
- Endoplasmic Reticulum Stress and Disease
- FOXO transcription factor regulation
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- RNA regulation and disease
- GABA and Rice Research
- MicroRNA in disease regulation
- Cellular transport and secretion
- Muscle Physiology and Disorders
Massachusetts Institute of Technology
2015-2024
Howard Hughes Medical Institute
2015-2024
McGovern Institute for Brain Research
2009-2024
IIT@MIT
1983-2018
Bethesda University
2005-2013
Seattle University
2005-2013
Pasadena City College
2005-2013
Advisory Board Company (United States)
2008-2013
Ithaca College
2013
New England Biolabs (United States)
2011
Amyotrophic lateral sclerosis (ALS) is a fatal degenerative motor neuron disorder. Ten percent of cases are inherited; most involve unidentified genes. We report here 13 mutations in the fused sarcoma/translated liposarcoma (FUS/TLS) gene on chromosome 16 that were specific for familial ALS. The FUS/TLS protein binds to RNA, functions diverse processes, and normally located predominantly nucleus. In contrast, mutant forms accumulated cytoplasm neurons, pathology similar TAR DNA-binding 43...
MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation miRNAs play essential roles embryonic development. We determined the temporal and spatial patterns of 115 conserved vertebrate zebrafish embryos microarrays situ hybridizations, using locked-nucleic acid–modified oligonucleotide probes. Most were expressed a highly tissue-specific manner during segmentation later stages,...
Mutations in the Caenorhabditis elegans genes lin-14 , lin-28 and lin-29 cause heterochronic developmental defects: timing of specific events several tissues is altered relative to other tissues. These defects result from temporal transformations fates cells, that is, certain cells express normally expressed by generated at stages. The identification characterization can be mutated heterochrony support proposal a mechanism for phylogenetic change suggest cellular genetic bases variation.
Abstract Background MicroRNAs are a large new class of tiny regulatory RNAs found in nematodes, plants, insects and mammals. thought to act as post-transcriptional modulators gene expression. In invertebrates microRNAs have been implicated regulators developmental timing, neuronal differentiation, cell proliferation, programmed death fat metabolism. Little is known about the roles Results We isolated 18-26 nucleotide from developing rat monkey brains. From sequences these human genomes we...
ABSTRACT The frequency of males (5AA; XO) among the self progeny wild-type Caenorhabditis elegans hermaphrodites XX) is about one in 500. Fifteen him (for "high incidence males") mutations have been identified that increase this by a factor ten to 150, as result increased X-chromosome nondisjunction. define complementation groups, which mapped: nine are autosomal, and sex linked. Most mutants superficially wild type anatomy behavior; however, him-4 display gonadal abnormalities, unc-86...
ABSTRACT We have isolated 145 fertile mutants of C. elegans that are defective in egg laying and characterized 59 them genetically, behaviorally pharmacologically. These define 40 new genes called egl, for egg-lay ing abnormal. Most the other previously identified genes. The egl differ with respect to severity their egg-laying defects presence behavioral or morphological pleiotropies. defined four distinct categories based on responses pharmacological agents serotonin imipramine, which...
Development of the nematode Caenorhabditis elegans is highly reproducible and fate every somatic cell has been reported. We describe here a previously uncharacterized in C. elegans: we show that germ cells, which hermaphrodites can differentiate into sperm oocytes, also undergo apoptotic death. In adult hermaphrodites, over 300 cells die, using same execution machinery (ced-3, ced-4 ced-9) as described 131 deaths. However, this activated by distinct pathway, loss egl-1 function, inhibits...
The biogenic amines serotonin and octopamine are present in the nematode Caenorhabditis elegans . Serotonin, detected histochemically whole mounts, is localized two pharyngeal neurons that appear to be neurosecretory. Octopamine, identified radioenzymatically crude extracts, probably also a few neurons. Exogenous elicit specific opposite behavioral responses , suggesting these compounds function physiologically as antagonists.
ABSTRACT Ninety-five mutants of the nematode Caenorhabditis elegans altered in cell lineages vulva have been isolated on basis their displaying one two phenotypes, Vulvaless or Multivulva. In mutants, which define 12 genes, no is present. Multivulva ten more supernumerary vulva-like protrusions are located along ventral side animal. A single recessive mutation responsible for phenotypes most, but not all, these strains. Fifteen 22 genes represented by multiple alleles. We shown a variety...