A5060239040- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Renal and related cancers
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- 3D Printing in Biomedical Research
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Gene Regulatory Network Analysis
- Genetic Syndromes and Imprinting
- RNA modifications and cancer
- Genetic Mapping and Diversity in Plants and Animals
- Genetics, Aging, and Longevity in Model Organisms
- Bioinformatics and Genomic Networks
- Single-cell and spatial transcriptomics
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
Elixir Medical (United States)
2023-2025
Keio University
2014-2023
Centre d'Immunologie et des Maladies Infectieuses
2023
Keio University Hospital
2013-2017
National Institute on Aging
2007-2016
National Institutes of Health
2006-2016
Johns Hopkins University
2005-2015
Chinese Academy of Fishery Sciences
2015
Institute on Aging
2004-2013
Japan Science and Technology Agency
2013
Degradation of mRNA is one the key processes that control steady-state level gene expression. However, rate decay for majority genes not known. We successfully obtained 19 977 non-redundant by microarray analysis RNA samples from mouse embryonic stem (ES) cells. Median estimated half-life was 7.1 h and only <100 genes, including Prdm1, Myc, Gadd45 g, Foxa2, Hes5 Trib1, showed less than 1 h. In general, species with short were enriched among regulatory functions (transcription factors),...
The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing complete open reading frame (ORF) for every human mouse gene. initially used random strategy select clones from large number libraries diverse tissues. Candidate were chosen based on 5′-EST sequences, then fully sequenced high accuracy analyzed by algorithms developed this project. Currently, more than 11,000 10,000 genes are...
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes mouse model, we compared expression profiles of metaphase II oocytes collected from 5- to 6-week-old mice those 42- 45-week-old using NIA 22K 60-mer oligo microarray. Among approximately 11 000 genes whose transcripts were detected oocytes, about 5% (530) showed statistically significant changes, excluding possibility global decline transcript abundance....
We present the AGEMAP (Atlas of Gene Expression in Mouse Aging Project) gene expression database, which is a resource that catalogs changes as function age mice. The database includes for 8,932 genes 16 tissues age. found great heterogeneity amount transcriptional with different tissues. Some displayed large differences old mice, suggesting these may contribute strongly to organismal decline. Other showed few or no age, indicating strong levels homeostasis throughout life. Based on pattern...
cDNA microarray technology has been increasingly used to monitor global gene expression patterns in various tissues and cell types. However, applications mammalian development have hampered by the lack of appropriate collections, particularly for early developmental stages. To overcome this problem, a PCR-based library construction method was derive 52,374 expressed sequence tags from pre- peri-implantation embryos, embryonic day (E) 12.5 female gonad/mesonephros, newborn ovary. From these...
The analysis of RNA-Seq data from individual differentiating cells enables us to reconstruct the differentiation process and degree (in pseudo-time) each cell. Such analyses can reveal detailed expression dynamics functional relationships for differentiation. To further elucidate processes, more insight into gene regulatory networks is required. pseudo-time be regarded as time information and, therefore, single-cell are time-course with high resolution. Although useful inferring networks,...
B-type lamins, the major components of nuclear lamina, are believed to be essential for cell proliferation and survival. We found that mouse embryonic stem cells (ESCs) do not need any lamins self-renewal pluripotency. Although genome-wide lamin-B binding profiles correlate with reduced gene expression, such is directly required silencing in ESCs or trophectoderm cells. However, proper organogenesis. Defects spindle orientation neural progenitor migration neurons probably cause brain...
Embryonic stem cells (ESCs) are derived from mammalian embryos during the transition totipotency, when individual blastomeres can make all lineages, to pluripotency, they competent only embryonic lineages. ESCs maintained with inhibitors of MEK and GSK3 (2i) thought represent an embryonically restricted ground state. However, we observed heterogeneous expression extraembryonic endoderm marker Hex in 2i-cultured embryos, suggesting that 2i blocked development prior epiblast commitment....
Loss of imprinting (LOI) the insulin-like growth factor II gene ( IGF2 ) is an epigenetic alteration that results in a modest increase expression, and it present normal colonic mucosa about 30% patients with colorectal cancer. To investigate its role intestinal tumorigenesis, we created mouse model Igf2 LOI by crossing female H19 +/– mice male Apc +/Min mice. Mice developed twice as many tumors did control littermates. Notably, these also showed shift toward less differentiated epithelium,...
Mouse Tabby ( Ta ) and X chromosome-linked human EDA share the features of hypoplastic hair, teeth, eccrine sweat glands. We have cloned gene find it to be homologous gene. The is altered in two alleles with a point mutation or deletion. expressed developing teeth epidermis; no expression seen corresponding tissues from mice. genes both encode alternatively spliced forms; novel exons now extend 3′ end All transcripts recovered same 5′ exon. longest cDNA encodes 391-residue transmembrane...
Delayed implantation (embryonic diapause) occurs when the embryo at blastocyst stage achieves a state of suspended animation. During this period, growth is very slow, with minimal or no cell division. Nearly 100 mammals in seven different orders undergo delayed implantation, but underlying molecular mechanisms that direct process remain largely unknown. In mice, ovariectomy before preimplantation ovarian estrogen secretion on day 4 pregnancy initiates dormancy, which normally lasts for 1–2...
We have developed a program for microarray data analysis, which features the false discovery rate testing statistical significance and principal component analysis using singular value decomposition method detecting global trends of gene-expression patterns. Additional include variance with multiple methods error adjustment, correction cross-channel correlation two-color microarrays, identification genes specific to each cluster tissue samples, biplot tissues corresponding tissue-specific...
ABSTRACT Little is known about gene action in the preimplantation events that initiate mammalian development. Based on cDNA collections made from each stage egg to blastocyst, 25438 3′-ESTs were derived, and represent 9718 genes, half of them novel. Thus, a considerable fraction genes dedicated embryonic expression. This study reveals profound changes expression include transient induction transcripts at stage. These results raise possibility development driven by series stage-specific...
Although the POU transcription factor Oct3/4 is pivotal in maintaining self renewal of embryonic stem (ES) cells, little known its molecular mechanisms. We previously reported that N-terminal transactivation domain required for activation Lefty1 expression (H. Niwa, S. Masui, I. Chambers, A. G. Smith, and J. Miyazaki, Mol. Cell. Biol. 22:1526-1536, 2002). Here we test whether a direct target Oct3/4. identified an ES cell-specific enhancer upstream promoter contains binding sites Sox2. Unlike...
ES cells are defined as self-renewing, pluripotent cell lines derived from early embryos. Cultures of also characterized by the expression certain markers thought to represent state. However, despite widespread key such Oct4 and appearance a characteristic undifferentiated morphology, functional may only small fraction cultures grown under self-renewing conditions. Thus phenotypically "undifferentiated" consist heterogeneous population functionally distinct types. Here we use transgenic...
Large-scale gene expression profiling was performed on embryo-derived stem cell lines to identify molecular signatures of pluripotency and lineage specificity. Analysis pluripotent embryonic (ES) cells, extraembryonic-restricted trophoblast (TS) terminally-differentiated mouse embryo fibroblast (MEF) cells identified profiles unique each type, as well genes common only ES TS cells. Whereas most the MEF-specific had been characterized previously, majority (67%) ES-specific were novel did not...