A5101584052- Ion Transport and Channel Regulation
- Pancreatitis Pathology and Treatment
- Pancreatic function and diabetes
- Cystic Fibrosis Research Advances
- Pluripotent Stem Cells Research
- Pancreatic and Hepatic Oncology Research
- Gastrointestinal disorders and treatments
- CRISPR and Genetic Engineering
- Ion channel regulation and function
- IgG4-Related and Inflammatory Diseases
- Neuroendocrine Tumor Research Advances
- Pediatric Hepatobiliary Diseases and Treatments
- Renal and related cancers
- Neuroscience of respiration and sleep
- Electrolyte and hormonal disorders
- Cannabis and Cannabinoid Research
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Rheumatoid Arthritis Research and Therapies
- Neonatal Respiratory Health Research
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Gene Regulatory Network Analysis
- Congenital Ear and Nasal Anomalies
Keio University
2016-2025
Keio University Hospital
2017
Yamaguchi University
2013
Nagoya University
2002-2012
Nagoya University Hospital
2012
The University of Texas Southwestern Medical Center
2001-2006
Southwestern Medical Center
2002
VA Greater Los Angeles Healthcare System
2000
Tokyo Medical and Dental University
1999
The analysis of RNA-Seq data from individual differentiating cells enables us to reconstruct the differentiation process and degree (in pseudo-time) each cell. Such analyses can reveal detailed expression dynamics functional relationships for differentiation. To further elucidate processes, more insight into gene regulatory networks is required. pseudo-time be regarded as time information and, therefore, single-cell are time-course with high resolution. Although useful inferring networks,...
IgG4-sclerosing cholangitis (IgG4-SC) patients have an increased level of serum IgG4, dense infiltration IgG4-positive plasma cells with extensive fibrosis in the bile duct wall, and a good response to steroid therapy. However, it is not easy distinguish IgG4-SC from primary sclerosing cholangitis, pancreatic cancer, cholangiocarcinoma on basis cholangiographic findings alone because various features are similar those above progressive or malignant diseases.The Research Committee...
The SLC26 transporters are a family of mostly luminal Cl− and HCO3− transporters. transport mechanism the Cl−/HCO3− stoichiometry not known for any member family. To address these questions, we simultaneously measured fluxes current or membrane potential slc26a3 slc26a6 expressed in Xenopus laevis oocytes human embryonic kidney 293 cells. mediates coupled 2Cl−/1HCO3− exchanger. modulated apparent affinity extracellular exchange by slc26a3. Interestingly, replacement with NO3− SCN− uncoupled...
Mouse embryonic stem cells (mESCs) have a remarkable capacity to maintain normal genome stability and karyotype in culture. We previously showed that infrequent bursts of Zscan4 expression (Z4 events) are important for the maintenance telomere length mESCs. However, molecular details Z4 events remain unclear. Here we show involve unexpected transcriptional derepression heterochromatin regions usually silent. During event, see rapid rerepression leading burst transcription coincides with...
Pancreatic duct epithelium secretes a HCO3−-rich fluid by mechanism dependent on cystic fibrosis transmembrane conductance regulator (CFTR) in the apical membrane. However, exact role of CFTR remains unclear. One possibility is that HCO3− permeability provides pathway for efflux during maximal secretion. We have therefore attempted to measure electrodiffusive fluxes induced changes membrane potential across interlobular ducts isolated from guinea pig pancreas. This was done recording...
Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability five transcription factors (TFs) toward aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, NEUROD2. contrast to previous methods that have shortcomings their speed efficiency, a cocktail these TFs as synthetic mRNAs can differentiate hPSCs 7 days, judged by calcium...
Abstract The modified total Sharp score (mTSS) is often used as an evaluation index for joint destruction caused by rheumatoid arthritis. In this study, special findings (ankylosis, subluxation, and dislocation) are detected to estimate the efficacy of mTSS using deep neural networks (DNNs). proposed method detects classifies finger regions ensemble mechanism. This integrates multiple DNN detection models, specifically single shot multibox detectors, different training data each finding. For...
The renal cortical collecting duct (CCD) plays an important role in systemic acid-base homeostasis. β-intercalated cells secrete most of the HCO[Formula: see text], which is mediated by a luminal, DIDS-insensitive, Cl − /HCO[Formula: text] exchange. identity luminal exchanger matter debate. Anion isoform 4 (AE4) cloned from rabbit kidney was proposed to perform this function (Tsuganezawa H et al. J Biol Chem 276: 8180–8189, 2001). By contrast, it (Royaux IE Proc Natl Acad Sci USA 98:...
HCO3− secretion is a vital activity in cystic fibrosis transmembrane conductance regulator (CFTR)-expressing epithelia. However, the role of CFTR this not well understood. Simultaneous measurements membrane potential and pHi and/or current CFTRexpressing Xenopus oocytes revealed dynamic control Cl−/HCO3− permeability ratio, which regulated by external Cl– (Cl–o). Thus, reducing from 110 to 0–10 mm resulted expected increase potential, but with no corresponding OH– or influx. Approximately...
Abstract The derivation of kidney tissues from human pluripotent stem cells (hPSCs) and its application for replacement therapy in end-stage renal disease have been widely discussed. Here we report that consecutive transfections two sets synthetic mRNAs encoding transcription factors can induce rapid efficient differentiation hPSCs into tissues, termed induced nephron-like organoids (iNephLOs). first set - FIGLA, PITX2, ASCL1 TFAP2C, differentiated SIX2 + SALL1 nephron progenitor with 92%...
Pancreatic HCO 3 − and fluid secretion were studied by monitoring luminal pH (pH L ) volume simultaneously in interlobular duct segments isolated from guinea‐pig pancreas. The secretory rate flux estimated fluorescence images obtained following microinjection of BCECF‐dextran (70 kDa, 20 μM) into the lumen. Ducts filled initially with a Cl ‐rich solution swelled steadily (2.0 nl min −1 mm 2 when /CO was introduced, increased to 8.08. When replaced glucuronate, spontaneous reduced 75 %, did...
Aquaporin 12 (AQP12) is the most recently identified member of mammalian AQP family and specifically expressed in pancreatic acinar cells. In vitro expression studies have revealed that AQP12 localized at intracellular sites. To determine physiological roles pancreas, we generated knockout mice for this gene (AQP12-KO). No obvious differences were observed under normal conditions between wild-type (WT) AQP12-KO terms growth, blood chemistry, fluid content, or histology. However, when induced...
The secretin-stimulated human pancreatic duct secretes HCO 3 − -rich fluid essential for normal digestion. Optimal stimulation of secretion likely requires coupled activities the cystic fibrosis transmembrane regulator (CFTR) anion channel and apical SLC26 Cl /HCO exchangers. However, whereas stimulated guinea pig ducts secrete ∼140 mM or more, mouse rat ∼40–70 . Moreover, axial distribution physiological roles exchangers in secretory processes remain controversial may vary among mammalian...
Harnessing epigenetic regulation is crucial for the efficient and proper differentiation of pluripotent stem cells (PSCs) into desired cell types. Histone H3 lysine 27 trimethylation (H3K27me3) functions as a barrier against through suppression developmental gene expression in PSCs. Here, we have generated human PSC (hPSC) lines which genome-wide reduction H3K27me3 can be induced by ectopic catalytic domain histone demethylase JMJD3 (called JMJD3c). We found that transient, forced...
In the present work, we characterized H(+) and HCO3- transport mechanisms in submandibular salivary gland (SMG) ducts of wild type, NHE2-/-, NHE3-/-, NHE2-/-;NHE3-/- double knock-out mice. The bulk recovery from an acid load across luminal membrane (LM) duct was mediated by a Na(+)-dependent HOE ethyl-isopropyl-amiloride (EIPA)-inhibitable 4,4'-diisothiocyanostilbene-2,2'-disulfonic (DIDS)-insensitive mechanism. increased rate pH(i) but did not change inhibition EIPA or insensitivity to...
Most epithelia that express CFTR secrete fluid rich in HCO3- and poor Cl- is generated by a CFTR-dependent absorption secretion process when aberrant leads to human diseases such as cystic fibrosis congenital chloride diarrhoea. Epithelial require expression of other transporters the luminal membrane secreting cells. Recent advances understanding this critical epithelial function revealed are members SLC26 family. Characterization several family reveals all characterized thus far...