A5100455468- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Plant Molecular Biology Research
- Aquaculture disease management and microbiota
- Plant nutrient uptake and metabolism
- Bacteriophages and microbial interactions
- Potato Plant Research
- Genetic diversity and population structure
- Parasite Biology and Host Interactions
- Studies on Chitinases and Chitosanases
- Insect Resistance and Genetics
- Soybean genetics and cultivation
- Molecular Biology Techniques and Applications
- Biosensors and Analytical Detection
- Microbial Inactivation Methods
- Insects and Parasite Interactions
- Natural Products and Biological Research
- Diatoms and Algae Research
- RNA and protein synthesis mechanisms
Cornell University
2014-2024
Robert W. Holley Center for Agriculture & Health
2014-2024
Huazhong Agricultural University
2015-2024
Tsinghua University
2021
Massachusetts General Hospital
2021
Harvard University Press
2021
Ningxia Medical University General Hospital
2021
Ningxia Medical University
2021
Binzhou Medical College Hospital
2021
Tang Du Hospital
2021
Abstract Plant-defense responses are triggered by perception of conserved microbe-associated molecular patterns (MAMPs), for example, flagellin or peptidoglycan. However, it remained unknown whether plants can detect derived from plant-parasitic animals, including nematodes. Here we show that several genera nematodes produce small molecules called ascarosides, an evolutionarily family nematode pheromones. Picomolar to micromolar concentrations ascr#18, the major ascaroside in nematodes,...
ABSTRACT Little is known about gene action in the preimplantation events that initiate mammalian development. Based on cDNA collections made from each stage egg to blastocyst, 25438 3′-ESTs were derived, and represent 9718 genes, half of them novel. Thus, a considerable fraction genes dedicated embryonic expression. This study reveals profound changes expression include transient induction transcripts at stage. These results raise possibility development driven by series stage-specific...
SUMMARY The Hg-SYV46 parasitism gene is expressed exclusively in the dorsal oesophageal gland cell of parasitic stages soybean cyst nematode, Heterodera glycines, and it encodes a secretory protein that contains C-terminal motif CLAVATA3/ESR-related (CLE) family Arabidopsis thaliana. In shoot floral meristems Arabidopsis, stem cells secret CLV3, founding member CLE family, activates CLV1/CLV2 receptor complex negatively regulates WUSCHEL expression to restrict size population. Mis-expression...
Evidence is emerging that plant-parasitic nematodes can secrete effectors to interfere with the host immune response, but it remains unknown how these conquer responses. Here, we depict a novel effector, MjTTL5, could suppress plant response. Immunolocalization and transcriptional analyses showed MjTTL5 expressed specifically within subventral gland of Meloidogyne javanica up-regulated in early parasitic stage nematode. Transgenic Arabidopsis lines expressing were significantly more...
Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ESR (CLE)-like effector proteins. These proteins have been shown to act as ligand mimics of plant CLE peptides and are required for successful nematode infection; however, the receptors CLE-like not identified. Here we demonstrate that CLV2 CORYNE (CRN), members receptor kinase family, signaling. Exogenous peptide assays overexpression CLEs in Arabidopsis demonstrated CRN perception CLEs. In addition, promoter-reporter showed both...
Abstract Microorganisms and nematodes in the rhizosphere profoundly impact plant health, small-molecule signaling is presumed to play a central role interactions. However, nature of signals underlying mechanisms are poorly understood. Here we show that ascaroside ascr#18, pheromone secreted by plant-parasitic nematodes, metabolized plants generate chemical repel reduce infection. Comparative metabolomics tissues excretions revealed ascr#18 converted into shorter side-chained ascarosides...
Secretions from the esophageal gland cells of plantparasitic nematodes play critical roles in nematodeparasitic cycle. A novel method to isolate cDNA encoding putative nematode secretory proteins was developed that utilizes mRNA for reverse transcription-polymerase chain reaction derived microaspiration cell contents parasitic stages soybean cyst Heterodera glycines. The resulting H. glycines cloned into pRK18 vector, and plasmid DNA transformed a mutated yeast host specific selection...
Cyst nematodes and root-knot elaborately transform cells within the vascular cylinders of plant roots into enlarged, multinucleate, metabolically active feeding cells. The giant are formed by repeated karyokinesis uncoupled from cytokinesis, whereas syncytia cyst arise coordinated cell wall dissolution coalescing cytoplasm adjacent Both undergo extensive architectural modifications, including thickening formation numerous ingrowths that increase plasmalemma surface area for solute uptake....
Featured paper: See Editorial p877
Plant CLAVATA3/ESR-related (CLE) peptides have diverse roles in plant growth and development. Here, we report the isolation functional characterization of five new CLE genes from potato cyst nematode Globodera rostochiensis. Unlike typical that contain a single motif, four Gr-CLE encode proteins with multiple motifs. These were found to be specifically expressed within dorsal esophageal gland cell parasitic stages, suggesting role for their encoded parasitism. Overexpression phenotypes...
SUMMARY In this article, we present the cloning of two CLAVATA3/ESR ( CLE )‐like genes, HsCLE1 and HsCLE2 , from beet cyst nematode Heterodera schachtii a plant‐parasitic with relatively broad host range that includes model plant Arabidopsis. CLEs are small secreted peptide ligands play important roles in growth development. By secreting mimics CLEs, can developmentally reprogramme root cells for formation unique feeding sites within roots its own benefit. Both encode polypeptides conserved...
Nematode effector proteins originating from esophageal gland cells play central roles in suppressing plant defenses and formation of the feeding that are required for growth development cyst nematodes. A gene (GrUBCEP12) encoding a unique ubiquitin carboxyl extension protein (UBCEP) consists signal peptide secretion, mono-ubiquitin domain, 12 amino acid (CEP12) domain was cloned potato nematode Globodera rostochiensis. This GrUBCEP12 expressed exclusively within nematode's dorsal cell,...
Abstract Nematodes that parasitize plant roots cause huge economic losses and have few mechanisms for control. Many parasitic nematodes infect plants by reprogramming root development to drive the formation of feeding structures. How take control is largely unknown. Here, we identify two host factors involved in function a receptor ligand mimic, GrCLE1, secreted potato cyst nematode Globodera rostochiensis. GrCLE1 correctly processed an active form proteases. Processed peptides bind directly...
Stem cell pools in the SAM (shoot apical meristem), RAM (root meristem) and vascular procambium/cambium are regulated by CLE-receptor kinase-WOX signaling modules. Previous data showed that cyst nematode CLE-like effector proteins delivered into host cells through a stylet, act as ligand mimics of plant A-type CLE peptides pivotal for successful parasitism. Here we report identification new class from nematodes with functional similarity to B-type peptide TDIF (tracheary element...
Root-knot nematodes (RKNs) infect many annual and perennial crops are the most devastating soil-born pests in vineyards. To develop a biotech-based solution for controlling RKNs grapes, we evaluated efficacy of plant-derived RNA interference (RNAi) silencing conserved RKN effector gene, 16D10, nematode resistance transgenic grape hairy roots. Two hairpin-based constructs, containing stem sequence 42 bp (pART27-42) or 271 (pART27-271) 16D10 were transformed into roots compared their small...
Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ENDOSPERM SURROUNDING REGION (CLE)-like effector proteins. These proteins act as ligand mimics of plant CLE peptides and are required for successful nematode infection. Previously, we showed that the CLV2/CORYNE (CRN) heterodimer receptor complex is signaling. However, there was only a partial reduction in infection when this signaling disrupted, indicating might be additional receptors. In study, demonstrate CLV1 RECEPTOR-LIKE PROTEIN...
The potato cyst nematode, Globodera rostochiensis, is an important pest of potato. Like other pathogens, plant parasitic nematodes are presumed to employ effector proteins, secreted into the apoplast as well host cytoplasm, alter cellular functions and successfully infect their hosts. We have generated a library ORFs encoding putative G. rostochiensis apoplastic effectors in vectors for expression planta. These clones were assessed morphological developmental effects on plants ability induce...
Like other biotrophic plant pathogens, plant-parasitic nematodes secrete effector proteins into host cells to facilitate infection. Effector that mimic CLAVATA3/ENDOSPERM SURROUNDING REGION-related (CLE) have been identified in several cyst nematodes, including the potato nematode (PCN); however, mechanistic details of this cross-kingdom mimicry are poorly understood. Plant CLEs posttranslationally modified and proteolytically processed function as bioactive ligands critical various aspects...
Potato cyst nematodes (PCNs), including Globodera rostochiensis (Woll.), are important pests of potato. Plant parasitic produce multiple effector proteins, secreted from their stylets, to successfully infect hosts. These include proteins delivered the apoplast and host cytoplasm. A number effectors G. predicted be cytoplasm have been identified, several belonging SPRY domain (SPRYSEC) family. SPRYSEC unique members genus implicated in both induction repression defense responses. We tested...
Abstract An international consortium is sequencing the euchromatic genespace of Medicago truncatula. Extensive bioinformatic and database resources support marker-anchored bacterial artificial chromosome (BAC) strategy. Existing physical genetic maps deep BAC-end help to guide effort, while EST databases provide essential for genome annotation as well transcriptome characterization microarray design. Finished BAC sequences are joined into overlapping sequence assemblies undergo an automated...