A5081175793- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- GABA and Rice Research
- Metabolomics and Mass Spectrometry Studies
- Insect Utilization and Effects
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- RNA regulation and disease
- Adipose Tissue and Metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Nematode management and characterization studies
- Olfactory and Sensory Function Studies
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Biofuel production and bioconversion
- Biochemical Acid Research Studies
- Enzyme function and inhibition
- Parasite Biology and Host Interactions
- Plant tissue culture and regeneration
- Birth, Development, and Health
- Retinal Imaging and Analysis
- Retinal Diseases and Treatments
- Plant Parasitism and Resistance
- Hormonal Regulation and Hypertension
Cornell University
2017-2024
Nanjing Normal University
2023
First Affiliated Hospital of Kunming Medical University
2021
Kunming Medical University
2021
Ithaca College
2018-2020
Cultivated maize (Zea mays) has retained much of the genetic diversity its wild ancestors. Here, we performed nontargeted liquid chromatography-mass spectrometry metabolomics to analyze metabolomes 282 inbred lines in Goodman Diversity Panel. This analysis identified a bimodal distribution foliar metabolites. Although 15% detected mass features were present >90% lines, majority found <50% samples. Whereas leaf bases and tips differentiated by flavonoid abundance, varieties (stiff-stalk,...
Untargeted metabolomics via high-resolution mass spectrometry can reveal more than 100,000 molecular features in a single sample, many of which may represent unidentified metabolites, posing significant challenges to data analysis. We here introduce Metaboseek, an open-source analysis platform designed for untargeted comparative and demonstrate its utility by uncovering biosynthetic functions conserved fat metabolism pathway, α-oxidation, using C. elegans as model. Metaboseek integrates...
Abstract Microorganisms and nematodes in the rhizosphere profoundly impact plant health, small-molecule signaling is presumed to play a central role interactions. However, nature of signals underlying mechanisms are poorly understood. Here we show that ascaroside ascr#18, pheromone secreted by plant-parasitic nematodes, metabolized plants generate chemical repel reduce infection. Comparative metabolomics tissues excretions revealed ascr#18 converted into shorter side-chained ascarosides...
Peroxisomal β-oxidation (pβo) is a highly conserved fat metabolism pathway involved in the biosynthesis of diverse signaling molecules animals and plants. In Caenorhabditis elegans, pβo required for ascarosides, that control development, lifespan, behavior this model organism. Via comparative mass spectrometric analysis mutants wildtype, we show C. elegans satellite P. pacificus contributes to life stage-specific several hundred previously unknown metabolites. The pβo-dependent portion...
Summary The production and regulation of defensive specialized metabolites play a central role in pathogen resistance maize ( Zea mays ) other plants. Therefore, identification genes involved plant metabolism can contribute to improved disease resistance. We used comparative metabolomics identify previously unknown antifungal seedling roots, investigated the genetic physiological mechanisms underlying their natural variation using quantitative trait locus mapping transcriptomics approaches....
It is now established that the central nervous system plays an important role in regulating whole body metabolism and energy balance. However, extent to which sensory systems relay environmental information modulate metabolic events peripheral tissues has remained poorly understood. In addition, it been challenging map molecular mechanisms underlying discrete modalities with respect their lipid metabolism. previous work our lab identified instructive roles for serotonin signaling as a...
Biogenic amine neurotransmitters play a central role in metazoan biology, and both their chemical structures cognate receptors are evolutionarily conserved. Their primary roles cell-to-cell signaling, as biogenic amines not normally recruited for communication between separate individuals. Here, we show that the nematode C. elegans, neurotransmitter-sensing G protein-coupled receptor, TYRA-2, is required avoidance responses to osas#9, an ascaroside pheromone incorporates neurotransmitter,...
A versatile synthesis of modular ascarosides, a family signaling molecules from Caenorhabditis elegans and other nematodes, via hydrogenolysis cyclic sulfate derived methyl-α-l-rhamnopyranoside is reported. The route enables selective introduction different side chains at the 1, 2, 4 positions sugar, as demonstrated for ascarosides C. Pristionchus pacificus. Bioassays with synthetic samples 4′-tigloyl ascaroside mbas#3 revealed its role an avoidance or dispersal signal.
Untargeted metabolomics indicates that the number of unidentified small-molecule metabolites may exceed protein-coding genes for many organisms, including humans, by orders magnitude. Uncovering underlying metabolic networks is essential elucidating physiological and ecological significance these biogenic small molecules. Here we develop a click-chemistry-based enrichment strategy, DIMEN (deep interrogation metabolism via enrichment), apply to investigate ascarosides, family signaling...
Many bacterivorous and parasitic nematodes secrete signaling molecules called ascarosides that play a central role regulating their behavior development. Combining stable-isotope labeling mass spectrometry-based comparative metabolomics, here we show are taken up from the environment metabolized by wide range of phyla, including plants, fungi, bacteria, mammals, as well nematodes. In most tested eukaryotes some into derivatives with shortened fatty acid side chains, analogous to ascaroside...
Abstract Fatty acid desaturation is central to metazoan lipid metabolism and provides building blocks of membrane lipids precursors diverse signaling molecules. Nutritional conditions associated microbiota regulate desaturase expression, but the underlying mechanisms have remained unclear. Here, we show that endogenous microbiota-dependent small molecule signals promote via nuclear receptor NHR-49/PPARα in C. elegans . Untargeted metabolomics a β-oxidation mutant, acdh-11 , which expression...
Cell-nonautonomous effects of signaling in the nervous system animals can influence diverse aspects organismal physiology. We previously showed that phosphorylation Ser49 α-subunit eukaryotic translation initiation factor 2 (eIF2α) two chemosensory neurons by PEK-1/PERK promotes entry Caenorhabditis elegans into dauer diapause. Here, we identified and characterized molecular determinants confer sensitivity to neuronal eIF2α on development physiology C. Isolation characterization mutations...
Identification of pheromone receptors plays a central role for uncovering signaling pathways that underlie chemical communication in animals. Here, we describe the synthesis and bioactivity photoaffinity probes ascaroside ascr#8, sex-pheromone model nematode, Caenorhabditis elegans. Structure-activity studies guided incorporation alkyne- diazirine-moieties revealed addition functionality sidechain ascr#8 was well tolerated, whereas modifications to ascarylose moiety resulted loss biological...
An antitumour lectin named AAL has been purified from the fruiting body of edible mushroom Agrocybe aegerita. In addition to having a distinct bioactivity, shows strong inhibition effects on human and mouse tumour cells. It shown that exerts its via apoptosis-induction. AAL-lactose complex have crystallized their diffraction data were collected with resolution 2.6 A 3.0 A, respectively. Both crystals belong space group P 6122 unit cell parameters = 123.98 b c 56.86 alpha= beta=90 degrees,...
Purpose: To investigate the clinical significance of macular estimated retinal ganglion cell (mRGC) and (eRGC) in diagnosis staging glaucoma. Methods: This is a cross-section study. All enrolled subjects underwent standard automated perimetry (SAP) optical coherence tomography (OCT) examination. Swedish Interactive Threshold Algorithm (SITA)-FAST detection strategy 24-2, 10-2 programs were employed SAP assessment. The visual-field parameters OCT calculated according to three formulas obtain...
One Sentence Summary HPLC-MS metabolite profiling of maize seedlings, in combination with genome-wide association studies, identifies numerous quantitative trait loci that influence the accumulation foliar metabolites. Abstract Cultivated ( Zea mays ) retains much genetic and metabolic diversity its wild ancestors. Non-targeted metabolomics using a diverse panel 264 inbred lines identified bimodal distribution prevalence Although 15% detected mass features were present >90% lines,...
Abstract Biogenic amine neurotransmitters play a central role in metazoan biology, and both their chemical structures cognate receptors are evolutionarily conserved. Their primary roles intra-organismal signaling, whereas biogenic amines not normally recruited for communication between separate individuals. Here, we show that C. elegans , neurotransmitter-sensing G protein-coupled receptor, TYRA-2, is required avoidance responses to osas#9, an ascaroside pheromone incorporates the...
ABSTRACT Fatty acid desaturation is central to metazoan lipid metabolism and provides building blocks of membrane lipids precursors diverse signaling molecules. Nutritional conditions associated microbiota regulate desaturase expression 1–4 , but the underlying mechanisms have remained unclear. Here, we show that endogenous microbiota-dependent small molecule signals promote via nuclear receptor NHR-49/PPARα in C. elegans . Untargeted metabolomics a β-oxidation mutant, acdh-11 which...
Summary The production and regulation of defensive specialized metabolites plays a central role in pathogen resistance maize ( Zea mays ) other plants. Therefore, identification genes involved plant metabolism can contribute to improved disease resistance. We used comparative metabolomics identify previously unknown antifungal seedling roots, investigated the genetic physiological mechanisms underlying their natural variation using quantitative trait locus (QTL) mapping transcriptomics...