A5009460838- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Biochemical Acid Research Studies
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- bioluminescence and chemiluminescence research
- Neuroendocrine regulation and behavior
- Adipose Tissue and Metabolism
- Photochromic and Fluorescence Chemistry
- Microbial Metabolic Engineering and Bioproduction
- RNA modifications and cancer
- Biochemical and Molecular Research
- GABA and Rice Research
- Birth, Development, and Health
- Chemical Synthesis and Analysis
- Genetics and Neurodevelopmental Disorders
- Genomics, phytochemicals, and oxidative stress
- Enzyme Catalysis and Immobilization
- Mitochondrial Function and Pathology
- Coenzyme Q10 studies and effects
- Lysosomal Storage Disorders Research
- Metabolomics and Mass Spectrometry Studies
Cornell University
2020-2024
Boston College
2017-2021
Abstract Recent studies of animal metabolism have revealed large numbers novel metabolites that are involved in all aspects organismal biology, but it is unclear to what extent metabolomes differ between sexes. Here, using untargeted comparative metabolomics for the analysis wildtype animals and sex determination mutants, we show C. elegans hermaphrodites males exhibit pervasive metabolomic differences. Several hundred small molecules produced exclusively or much larger amounts one sex,...
Abstract Viruses utilize distinct binding interactions with a variety of host factors to gain entry into cells. A chemical strategy is described precisely perturb specific molecular interaction between adeno‐associated virus and its cell, which can be rapidly reversed by light. This enables pausing the process at stage then restart it non‐invasive stimulus. The ability synchronize invading population discrete step in pathway will highly valuable for enabling facile experimental...
Signaling molecules derived from attachment of diverse metabolic building blocks to ascarosides play a central role in the life history C. elegans and other nematodes; however, many aspects their biogenesis remain unclear. Using comparative metabolomics, we show that pathway mediating formation intestinal lysosome-related organelles (LROs) is required for biosynthesis most modular as well previously undescribed glucosides. Similar ascarosides, glucosides are highly selective assembly...
Few nucleoside-derived natural products have been identified from animals, despite the ubiquity of nucleosides in living organisms. Here, we use a combination synthesis and emerging electron microscopy technique microcrystal diffraction to determine structures several
Article17 March 2022Open Access Source DataTransparent process CEST-2.2 overexpression alters lipid metabolism and extends longevity of mitochondrial mutants Antonia Piazzesi orcid.org/0000-0002-7591-6473 German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany Contribution: Conceptualization, Supervision, Validation, Investigation, Writing - original draft Search more papers by this author Yiru Wang orcid.org/0000-0002-6522-9995 Investigation Joshua Jackson...
The recently discovered modular glucosides (MOGLs) form a large metabolite library derived from combinatorial assembly of moieties amino acid, neurotransmitter, and lipid metabolism in the model organism C. elegans. Combining CRISPR-Cas9 genome editing, comparative metabolomics, synthesis, we show that carboxylesterase homologue Cel-CEST-1.2 is responsible for specific 2-O-acylation diverse glucose scaffolds with wide variety building blocks, resulting more than 150 different MOGLs. We...
Abstract The biosynthetic pathways and functions of ascaroside signaling molecules in the nematode Caenorhabditis elegans have been studied to better understand complex, integrative developmental decision-making. Although it is known that ascarosides play multiple roles development behavior species other than C. elegans, these parallel pheromone systems not well-studied. Here, we show briggsae are biosynthesized same manner as act induce alternative pathway generates stress-resistant dauer...
Abstract Fatty acid desaturation is central to metazoan lipid metabolism and provides building blocks of membrane lipids precursors diverse signaling molecules. Nutritional conditions associated microbiota regulate desaturase expression, but the underlying mechanisms have remained unclear. Here, we show that endogenous microbiota-dependent small molecule signals promote via nuclear receptor NHR-49/PPARα in C. elegans . Untargeted metabolomics a β-oxidation mutant, acdh-11 , which expression...
Abstract Viruses utilize distinct binding interactions with a variety of host factors to gain entry into cells. A chemical strategy is described precisely perturb specific molecular interaction between adeno‐associated virus and its cell, which can be rapidly reversed by light. This enables pausing the process at stage then restart it non‐invasive stimulus. The ability synchronize invading population discrete step in pathway will highly valuable for enabling facile experimental...
Reactive electrophilic intermediates such as coenzyme A esters play central roles in metabolism but are difficult to detect with conventional strategies. Here, we introduce hydroxylamine-based stable isotope labeling convert reactive into derivatives that easily detectable via LC–MS. In the model system Caenorhabditis elegans, parallel treatment 14NH2OH and 15NH2OH revealed >1000 labeled metabolites, e.g., derived from peptide, fatty acid, ascaroside pheromone biosyntheses. Results NH2OH of...
Nucleosides are essential cornerstones of life, and nucleoside derivatives synthetic analogues have important biomedical applications. Correspondingly, production non-canonical in animal model systems is particular interest. Here, we report the discovery diverse glucose-based nucleosides Caenorhabditis elegans related nematodes. Using a mass spectrometric screen based on all-ion fragmentation combination with total synthesis, show that C. selectively glucosylates series modified purines but...
Abstract Signaling molecules derived from attachment of diverse metabolic building blocks to ascarosides play a central role in the life history C. elegans and other nematodes; however, many aspects their biogenesis remain unclear. Using comparative metabolomics, we show that pathway mediating formation intestinal lysosome-related organelles (LROs) is required for biosynthesis most modular as well previously undescribed glucosides. Similar ascarosides, glucosides are highly selective...
ABSTRACT Fatty acid desaturation is central to metazoan lipid metabolism and provides building blocks of membrane lipids precursors diverse signaling molecules. Nutritional conditions associated microbiota regulate desaturase expression 1–4 , but the underlying mechanisms have remained unclear. Here, we show that endogenous microbiota-dependent small molecule signals promote via nuclear receptor NHR-49/PPARα in C. elegans . Untargeted metabolomics a β-oxidation mutant, acdh-11 which...
Abstract Recent studies of animal metabolism have revealed large numbers novel metabolites that are involved in all aspects organismal biology, but it is unclear to what extent metabolomes differ between sexes. Here, using untargeted comparative metabolomics for the analysis wildtype animals and a series germline mutants, we show C. elegans hermaphrodites males exhibit pervasive metabolomic differences. Several hundred small molecules produced exclusively or much larger amounts one sex,...