A5018186381- Mitochondrial Function and Pathology
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Genetics, Aging, and Longevity in Model Organisms
- ATP Synthase and ATPases Research
- Cancer Genomics and Diagnostics
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- Biochemical Acid Research Studies
- Parasitic Infections and Diagnostics
- Sphingolipid Metabolism and Signaling
- Probiotics and Fermented Foods
- CRISPR and Genetic Engineering
- Gastric Cancer Management and Outcomes
- Genetic factors in colorectal cancer
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Inflammatory Bowel Disease
- Research on Leishmaniasis Studies
- Pneumonia and Respiratory Infections
- Cancer Mechanisms and Therapy
- Insect symbiosis and bacterial influences
- Neurological diseases and metabolism
- Chromatin Remodeling and Cancer
- Pneumocystis jirovecii pneumonia detection and treatment
Bambino Gesù Children's Hospital
2022-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2023-2025
German Center for Neurodegenerative Diseases
2016-2024
University of Bonn
2020-2021
University of Padua
2014
Introduction Since the beginning of SARS-CoV-2 pandemic in early 2020, it has been apparent that children were partially protected from both infection and more severe forms disease. Many different mechanisms have proposed to explain this phenomenon, including children’s frequent exposure other upper respiratory infections vaccines, which inflammatory cytokines they are likely produce response infection. Furthermore, given presence intestine its ability infect enterocytes, combined with well...
Abstract Abnormal intraneuronal accumulation of soluble and insoluble α-synuclein (α-Syn) is one the main pathological hallmarks synucleinopathies, such as Parkinson’s disease (PD). It has been well documented that reversible liquid-liquid phase separation α-Syn can modulate synaptic vesicle condensates at presynaptic terminals. However, also form liquid-like droplets may convert into amyloid-enriched hydrogels or fibrillar polymorphs under stressful conditions. To advance our understanding...
Chromatin structure orchestrates the accessibility to genetic material. Replication-independent histone variants control transcriptional plasticity in postmitotic cells. The life-long accumulation of these histones has been described, yet implications on organismal aging remain elusive. Here, we study importance variant H3.3 Caenorhabditis elegans longevity pathways. We show that H3.3-deficient nematodes have negligible lifespan differences compared wild-type animals. However, is essential...
Impaired mitochondrial energy metabolism contributes to a wide range of pathologic conditions, including neurodegenerative diseases. Mitochondrial apoptosis-inducing factor (AIF) is required for the correct maintenance electron transport chain. An emerging body clinical evidence indicates that several mutations in AIFM1 gene are causally linked severe forms disorders. Here we investigate consequence WAH-1/AIF deficiency survival nematode Caenorhabditis elegans. Moreover, assess C. elegans...
Pneumonia caused by Pneumocystis jirovecii infection (PCP) is a potentially life-threatening illness, particularly affecting the immunocompromised. The past two decades have shown an increase in PCP incidence; however, underlying factors that promote disease severity and fatality yet to be fully elucidated. Recent evidence suggests microbiota of respiratory tract may play role stimulating or repressing pulmonary inflammation, as well progression both bacterial viral pneumonia. Here, we...
Abstract Background Many patients with Inflammatory Bowel Diseases do not respond to biological therapies. Extrachromosomal circular DNA(eccDNA) has been showed play a role in the pathophysiology of IBD, as there is higher presence eccDNA intestinal mucosa these patients. By characterizing gene content eccDNA, up-regulation certain genes observed; one NRG1 (Neuregulin 1), an EGF ligand that guides tissue repair after injury and associated plasticity. This located on chromosome 8p12 it...
Abstract Background The diagnosis of Inflammatory Bowel Disease, which continues to increase year by year, is complex; these disorders can be confirmed investigations such as fecal calprotectin, endoscopy, histology biopsies, and CT scan; despite this, there a lack highly specific minimally invasive techniques that allow not only but also determination the severity disease its evolution. Given challenges, search for reliable predictors direct clinician toward personalized patient care very...
Abstract Background Inflammatory Bowel Diseases (IBD), comprising Ulcerative Colitis (UC) and Crohn’s Disease (CD), are chronic, relapsing-remitting disorders of the gastrointestinal tract with unclear etiology. Identifying reliable biomarkers for predicting disease progression treatment response remains a priority. Extrachromosomal circular DNA (eccDNA) has shown promise as diagnostic prognostic biomarker in oncology, but its potential IBD patients, including gene fragments involved, not...
Cryptosporidium spp. are zoonotic protozoan parasites with a global prevalence, both gastrointestinal and pulmonary involvement. Though symptoms can often be relatively mild, they become severe even fatal in children under five, the elderly, immunocompromised individuals, making cryptosporidiosis leading cause of morbidity mortality fragile populations. Furthermore, there is an urgent clinical need for alternative therapies against cryptosporidiosis, as currently available FDA-approved...
Mutations in the AIFM1 gene have been identified recessive X-linked mitochondrial diseases. Functional and molecular consequences of these pathogenic mutations poorly studied vivo.
We have shown that sphingosine 1-phosphate (S1P) generated by kinase 2 (SK2) is toxic in neurons lacking S1P-lyase (SGPL1), the enzyme catalyzes its irreversible cleavage. Interestingly, patients harboring mutations gene encoding this (SGPL1) often present with neurological pathologies. Studies a mouse model developmental neural-specific ablation of SGPL1 (SGPL1fl/fl/Nes) confirmed importance S1P metabolism for presynaptic architecture and neuronal autophagy, known to be essential brain...
Aberrant mitochondrial function contributes to the pathogenesis of various metabolic and chronic disorders. Inhibition insulin/IGF-1 signaling (IIS) represents a promising avenue for treatment diseases, although many molecular mechanisms underlying this beneficial effect remain elusive. Using an unbiased multi-omics approach, we report here that IIS inhibition reduces protein synthesis favors catabolism in deficient Caenorhabditis elegans We unveil lifespan extension does not occur through...
Article17 March 2022Open Access Source DataTransparent process CEST-2.2 overexpression alters lipid metabolism and extends longevity of mitochondrial mutants Antonia Piazzesi orcid.org/0000-0002-7591-6473 German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany Contribution: Conceptualization, Supervision, Validation, Investigation, Writing - original draft Search more papers by this author Yiru Wang orcid.org/0000-0002-6522-9995 Investigation Joshua Jackson...
Mitochondria sustain the energy demand of cell. The composition and functional state mitochondrial oxidative phosphorylation system are informative indicators organelle bioenergetic capacity. Here, we describe a highly sensitive reproducible method for single-cell quantification CI- CIV-containing respiratory supercomplexes (CI∗CIV-SCs) as an alternative means assessing chain integrity. We apply proximity ligation assay (PLA) stain CI∗CIV-SCs in fixed human mouse brains, tumorigenic cells,...
Mitochondrial "retrograde" signaling may stimulate organelle biogenesis as a compensatory adaptation to aberrant activity of the oxidative phosphorylation (OXPHOS) system. To maintain energy-consuming processes in OXPHOS deficient cells, alternative metabolic pathways are functionally coupled degradation, recycling and redistribution biomolecules across distinct intracellular compartments. While transcriptional regulation mitochondrial network expansion has been focus many studies, molecular...
ABSTRACT The present study aimed to investigate the pharyngeal and nasal microbiota composition in children with adenotonsillar hypertrophy (AH) assess longitudinal alterations both after a probiotic oral spray treatment. A cohort of 57 AH patients were enrolled randomly assigned placebo groups for 5-month treatment course. Pharyngeal swabs collected before analyzed by 16S rRNA-based metataxonomics axenic cultures pathobiont identification. rRNA sequences from 65 healthy (HC) used as...