A5002697232- Cystic Fibrosis Research Advances
- Bacterial biofilms and quorum sensing
- Infections and bacterial resistance
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Antifungal resistance and susceptibility
- Pediatric health and respiratory diseases
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Nematode management and characterization studies
- Antimicrobial Peptides and Activities
- Streptococcal Infections and Treatments
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Inhalation and Respiratory Drug Delivery
- Microbial infections and disease research
- Respiratory viral infections research
- Enterobacteriaceae and Cronobacter Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Infectious Diseases and Mycology
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2024
Bambino Gesù Children's Hospital
2015-2024
Cystic Fibrosis Trust
2021
Centro Diagnostico Italiano
2021
Boston Children's Hospital
2012-2018
University of Manchester
2017
National Agency for New Technologies, Energy and Sustainable Economic Development
2016
ENEA Casaccia Research Centre
2016
Istituto Giannina Gaslini
2008-2011
Cystic Fibrosis Research Foundation
2011
ABSTRACT The alarming diffusion of multidrug-resistant (MDR) bacterial strains requires investigations on nonantibiotic therapies. Among such therapies, the use bacteriophages (phages) as antimicrobial agents, namely, phage therapy, is a promising treatment strategy supported by findings recent successful compassionate treatments in Europe and United States. In this work, we combined host range genomic information to design 6-phage cocktail killing several clinical Pseudomonas aeruginosa ,...
Abstract Dysregulated inflammasome activation contributes to respiratory infections and pathologic airway inflammation. Through basic translational approaches involving murine models human genetic epidemiology, we show here the importance of different inflammasomes in regulating inflammatory responses mice humans with cystic fibrosis (CF), a life-threatening disorder lungs digestive system. While both contributing pathogen clearance, NLRP3 more than NLRC4 deleterious CF correlates defective...
ABSTRACT We report a case of fatal pulmonary infection caused by Mycobacterium abscessus in young patient with cystic fibrosis, who underwent bipulmonary transplantation after 1-year history severe lung disease. Fifteen days surgery he developed septic fever progressive deterioration function. M. , initially isolated from pleural fluid specimen, was then recovered repeated blood samples, suggesting disseminated nature the mycobacterial Drug susceptibility testing assay, performed on two...
Abstract Background Stenotrophomonas maltophilia is emerging as one of the most frequently found bacteria in cystic fibrosis (CF) patients. In present study, phenotypic and genotypic traits a set 98 isolates S. obtained from clinical (CF non-CF patients) environmental sources were comparatively evaluated. Results exhibited high level genomic diversity both CF group, thus possibly allowing this bacterium to expand its pathogenic potentials. Strains sharing same pulsotype infected different...
T helper 9 (Th9) cells contribute to lung inflammation and allergy as sources of interleukin-9 (IL-9). However, the mechanisms by which IL-9/Th9 mediate immunopathology in are unknown. Here we report an IL-9-driven positive feedback loop that reinforces allergic inflammation. We show IL-9 increases IL-2 production mast cells, leads expansion CD25+ type 2 innate lymphoid (ILC2) subsequent activation Th9 cells. Blocking or inhibiting CD117 (c-Kit) signalling counteracts pathogenic effect...
Background Cystic fibrosis (CF) is a disorder affecting the respiratory, digestive, reproductive systems and sweat glands. This lethal hereditary disease has known or suspected links to dysbiosis gut microbiota. High-throughput meta-omics-based approaches may assist in unveiling this complex network of symbiosis modifications. Objectives The aim study was provide predictive functional model microbiota enterophenotype pediatric patients affected by CF under clinical stability. Methods...
The long-term use of antibiotics has led to the emergence multidrug-resistant bacteria. A promising strategy combat bacterial infections aims at hampering their adaptability host environment without affecting growth. In this context, intercellular communication system quorum sensing (QS), which controls virulence factor production and biofilm formation in diverse human pathogens, is considered an ideal target. Here, we describe identification new inhibitors pqs QS pathogen Pseudomonas...
Inhaled siRNA therapy has a unique potential for treatment of severe lung diseases, such as cystic fibrosis (CF). Nevertheless, drug delivery system tackling barriers is mandatory to enhance gene silencing efficacy in the airway epithelium. We recently demonstrated that lipid-polymer hybrid nanoparticles (hNPs), comprising poly(lactic-co-glycolic) acid (PLGA) core and lipid shell dipalmitoyl phosphatidylcholine (DPPC), may assist transport nucleic cargo through mucus-covered human To study...
Stenotrophomonas maltophilia has recently gained considerable attention as an important emerging pathogen in cystic fibrosis (CF) patients. However, the role of this microorganism pathophysiology CF lung disease remains largely unexplored. In present study for first time we assessed ability S. isolates to adhere and form biofilm experimental infection experiments using CF-derived bronchial epithelial IB3-1cell line. The flagella on adhesiveness IB3-1 cell monolayers was also by fliI mutant...
The present study was undertaken in order to understand more about the interaction occurring between S. maltophilia and P. aeruginosa, which are frequently co-isolated from CF airways. For this purpose, RR7 aeruginosa RR8 strains, lung of a chronically infected patient during pulmonary exacerbation episode, were evaluated for reciprocal effect planktonic growth, adhesion biofilm formation onto both polystyrene bronchial cell monolayer, motility, as well gene expression mixed biofilms....
Stenotrophomonas maltophilia has been recognized as an emerging multi-drug resistant opportunistic pathogen in cystic fibrosis (CF) patients. We report a comparative genomic and phenotypic analysis of 91 S. strains from 10 CF patients over 12-year period. Draft genome analyses included silico Multi-Locus Sequence Typing (MLST), Single-Nucleotide Polymorphisms (SNPs), pangenome characterization. Growth rate, biofilm formation, motility, mutation frequency, vivo virulence, vitro antibiotic...
Abstract Background Treatment of cystic fibrosis-associated lung infections is hampered by the presence multi-drug resistant pathogens, many which are also strong biofilm producers. Antimicrobial peptides, essential components innate immunity in humans and animals, exhibit relevant vitro antimicrobial activity although they tend not to select for strains. Results Three α-helical BMAP-27 BMAP-28 bovine origin, artificial P19(9/B) peptide were tested, comparatively Tobramycin, their...
<h3>Objective</h3> To investigate the accuracy of procalcitonin (PCT) as a diagnostic marker nosocomial sepsis (NS) and define most accurate cut-off to distinguish infected from uninfected neonates. <h3>Setting</h3> Six neonatal intensive care units (NICUs). <h3>Patients</h3> 762 neonates admitted six NICUs during 28-month observational study for whom at least one serum sample was taken on admission. <h3>Main outcome measures</h3> Positive negative predictive values different PCT levels....
Cystic fibrosis (CF) is a genetic disease resulting in chronic polymicrobial infections of the airways and progressive decline lung function. To gain insight into underlying causes severe diseases, we aimed at comparing airway microbiota detected sputum CF patients with stable function (S) versus those substantial (SD). Microbiota composition was investigated by using culture-based culture-independent methods, performing multivariate statistical analyses. Culture-based methods identified...
The present study was carried out to understand the adaptive strategies developed by Stenotrophomonas maltophilia for chronic colonization of cystic fibrosis (CF) lung. For this purpose, 13 temporally isolated strains from a single CF patient chronically infected over 10-year period were systematically characterized growth rate, biofilm formation, motility, mutation frequencies, antibiotic resistance, and pathogenicity. Pulsed-field gel electrophoresis (PFGE) showed time presence two...
The rise in antibiotic resistance among bacterial pathogens has prompted the exploitation of alternative antibacterial strategies, such as antivirulence therapy. By inhibiting virulence traits, drugs are expected to lessen pathogenicity without affecting growth, therefore avoiding spread resistance. However, some studies argued against this assumption, and lack clinical use hampers empirical assessment concept. Here we compared mode action range activity two which have been proposed for...
The intrinsic antibiotic resistance of Stenotrophomonas maltophilia, along with its ability to form biofilm both on abiotic surfaces and host tissues, dramatically affects the efficacy therapy. In this work, 85 S. maltophilia strains isolated in several hospital central Italy from clinical settings were evaluated for their genetic relatedness (by pulsed-field gel electrophoresis, PFGE), formation microtiter plate assay), planktonic Kirby-Bauer disk diffusion technique). population showed a...
In recent years, next-generation sequencing (NGS) was employed to decipher the structure and composition of microbiota airways in cystic fibrosis (CF) patients. However, little is still known about overall gene functions harbored by resident microbial populations which specific genes are associated with various stages CF lung disease. present study, we aimed identify repertoire twelve patients severe normal/mild disease performing sputum shotgun metagenome sequencing. The abundance metabolic...