A5084331370- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Bacterial Identification and Susceptibility Testing
- Plant Pathogens and Fungal Diseases
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Resistance in Staphylococcus
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Nail Diseases and Treatments
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Virus-based gene therapy research
- Mycobacterium research and diagnosis
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Oral microbiology and periodontitis research
- Bacillus and Francisella bacterial research
- Bacterial biofilms and quorum sensing
- Complementary and Alternative Medicine Studies
- Biosensors and Analytical Detection
- Escherichia coli research studies
- Ocular Infections and Treatments
Agostino Gemelli University Polyclinic
2017-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2025
Università Cattolica del Sacro Cuore
2012-2024
Catholic University of America
2014
Czech Academy of Sciences, Institute of Microbiology
2011-2014
Istituto Superiore di Sanità
2004-2005
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin wild-type and fks mutant isolates Candida Aspergillus. Complete essential agreement observed with the CLSI reference method, categorical 94.1% tested. Thus, MALDI-TOF MS is a reliable accurate method detect fungal reduced susceptibility.
Culture-independent molecular techniques such as real-time PCRs offer the potential for early diagnosis of invasive aspergillosis (IA), thereby reducing disease-associated mortality rate. PCR-based testing is presently excluded from disease-defining consensus criteria due to lack standardization and clinical validation. A single-center prospective study was conducted investigate performance commercially available MycAssay Aspergillus test detecting DNA in patients with suspicion IA. To this...
ABSTRACT The widespread use of antifungal agents, which is likely to expand with their enhanced availability, has promoted the emergence drug-resistant strains. Antifungal susceptibility testing (AFST) now an essential procedure for guiding appropriate therapy. Recently, we developed a matrix-assisted laser desorption ionization–time flight mass spectrometry (MALDI-TOF MS)-based method that enables detection fungal isolates reduced echinocandin susceptibility, relying on proteome changes are...
Coinfections with bacteria or fungi may be a frequent complication of COVID-19, but coinfections Candida species in COVID-19 patients remain rare. We report the 53-day clinical course complicated type-2 diabetes patient diagnosed who developed bloodstream infections initially due to methicillin-resistant Staphylococcus aureus, secondly multidrug-resistant Gram-negative bacteria, and lastly possibly fatal glabrata. The development FKS-associated pan-echinocandin resistance C. glabrata...
ABSTRACT We evaluated the usefulness of matrix-assisted laser desorption ionization–time flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at species and subspecies levels by using an in-house database 25 reference cryptococcal spectra. Eighty-one out 82 isolates (72 neoformans 10 gattii ) tested were correctly identified with respect to their molecular type designations. showed that MALDI-TOF MS is a practicable alternative conventional mycology or DNA-based methods.
We report the first comparative evaluation between Bruker Biotyper MS (BMS) and Vitek (VMS) for identification of yeasts. The rate correct identifications at species level was comparable using commercial databases (89.8% versus 84.3%; P = 0.712), but higher BMS an in-house-extended database (100% 0.245). Importantly, misidentification significantly VMS (1% 12.1%; < 0.0001), including major errors (0% 4.5%; 0.0036).
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been evaluated for the identification multidrug-resistant Acinetobacter baumannii nosocomial outbreaks in comparison with repetitive sequence-based PCR DiversiLab system. The results suggest that MALDI-TOF MS can be used real-time detection before from DNA-based systems are available.
Objectives: To evaluate the magnetic resonance-based T2Bacteria Panel assay for direct detection of ESKAPEc (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli) pathogens in blood samples patients with suspected bloodstream infection (BSI).Patients methods: Adult admitted to Emergency Medicine Department, Infectious Diseases Unit ICU a large tertiary-care hospital were included if they had culture (BC)...
To determine the effects of a strategy that uses serum (1,3)-β-d-glucan (BDG) results for antifungal treatment ICU patients at high risk invasive candidiasis. Adult admitted to from January 2012 June 2014 were included if they exhibited sepsis time BDG testing and met Candida score components ≥3. A retrospective analysis collected data was performed. In total, 198 studied. Of 63 BDG-positive patients, 47 with candidaemia 16 probable infection, all [31.8% (63/198)] received therapy. 135...
Sensititre YeastOne (SYO) is an affordable alternative to the Clinical and Laboratory Standards Institute (CLSI) reference method for antifungal susceptibility testing. In this study, MICs of yeast isolates from 1,214 bloodstream infection episodes, generated by SYO during hospital laboratory activity (January 2005 December 2013), were reanalyzed using current CLSI clinical breakpoints/epidemiological cutoff values assign (or wild-type [WT] phenotype) systemic agents. Excluding Candida...
The most prevalent cause of nosocomial bloodstream infection (BSI) among non-C. albicans Candida species, parapsilosis, may not only be resistant to azole antifungal agents but also disseminate vulnerable patients. In this survey BSIs occurring at a large Italian hospital between May 2014 and 2019, C. parapsilosis accounted for 28.5% (241/844) all isolates causing BSI episodes. majority episodes (151/844) occurred in medical wards. Across the five yearly periods, rates non-susceptibility...
Fluconazole-resistant Candida parapsilosis is a matter of concern.
The genome of Shigella, a gram negative bacterium which is the causative agent bacillary dysentery, shares strong homologies with that its commensal ancestor, Escherichia coli. acquisition, by lateral gene transfer, large plasmid carrying virulence determinants has been crucial event in evolution towards pathogenic lifestyle and paralleled occurrence mutations affecting genes, negatively interfere expression factors. In this context, we have analysed to what extent presence plasmid-encoded...
ABSTRACT In recent studies evaluating the usefulness of matrix-assisted laser desorption ionization–time flight mass spectrometry (MALDI-TOF MS)-based identification yeasts for routine diagnosis fungal infections, preanalytical sample processing has emerged as a critical step reliable MALDI-TOF MS outcomes, especially when Bruker Daltonics Biotyper software was used. addition, inadequate results often occurred due to discrepancies between methods used clinical testing and database...
Abstract Background (1,3)-β- d -Glucan has been widely used in clinical practice for the diagnosis of invasive Candida infections. However, such serum biomarker showed potential to guide antimicrobial therapy order reduce duration empirical antifungal treatment critically ill septic patients with suspected candidiasis. Methods This was a single-centre, randomized, open-label trial which were enrolled during admission intensive care unit (ICU). All who presented infection risk factors and...
Recently, bergamot oil was shown to be a potent antifungal agent in vitro against clinically important Candida species. In this study, the activities of natural essence and its furocoumarin-free distilled extracts on dermatophytes such as Trichophyton, Microsporum Epidermophyton species were investigated.In susceptibility testing assays 92 clinical isolates (Trichophyton mentagrophytes n = 20, Trichophyton rubrum 18, interdigitale 15, tonsurans 2, canis 24, gypseum 1 floccosum 12) performed...
Abstract The echinocandins are relatively new antifungal drugs that represent, together with the older azoles, recommended and/or preferred agents to treat candidaemia and other forms of invasive candidiasis in human patients. If “time is essence” reduce mortality for these infections, administration appropriate therapy could be accelerated by timely reporting laboratory test results. In this study, we attempted validate a MALDI-ToF mass spectrometry-based assay nti f ungal s usceptibility t...
In this study we compare the capability of amplification fragment-length polymorphism (AFLP) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify subtype isolates members Candida parapsilosis complex (C. parapsilosis, C. orthopsilosis, metapsilosis) Lodderomyces elongisporus, which cannot be differentiated with biochemical methods. Both techniques correctly identified all included in clustered within different species. DNA-based...