A5036992786- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Advanced biosensing and bioanalysis techniques
- Vibrio bacteria research studies
- Extracellular vesicles in disease
- Microbial infections and disease research
- RNA Interference and Gene Delivery
- Respiratory viral infections research
- Zebrafish Biomedical Research Applications
- Circular RNAs in diseases
- Cystic Fibrosis Research Advances
- Plant Virus Research Studies
- MicroRNA in disease regulation
- Genomics, phytochemicals, and oxidative stress
- COVID-19 impact on air quality
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Air Quality and Health Impacts
- Muscle Physiology and Disorders
- Cancer-related molecular mechanisms research
- Skin Protection and Aging
- Neonatal Respiratory Health Research
- Atherosclerosis and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- Bacterial Genetics and Biotechnology
University of Milan
2018-2025
ABSTRACT The alarming diffusion of multidrug-resistant (MDR) bacterial strains requires investigations on nonantibiotic therapies. Among such therapies, the use bacteriophages (phages) as antimicrobial agents, namely, phage therapy, is a promising treatment strategy supported by findings recent successful compassionate treatments in Europe and United States. In this work, we combined host range genomic information to design 6-phage cocktail killing several clinical Pseudomonas aeruginosa ,...
Abstract Cystic fibrosis (CF) is a hereditary disease due to mutations in the CFTR gene and causes mortality humans mainly respiratory infections caused by Pseudomonas aeruginosa . In previous work we used phage therapy, which treatment with mix of phages, actively counteract acute P mice Galleria mellonella larvae. this apply therapy PAO1 CF zebrafish model. The structure channel evolutionary conserved between fish mammals cftr -loss-of-function embryos show phenotype that recapitulates...
In the last years, extracellular vesicles (EVs) from different plant matrices have been isolated and gained interest of scientific community for their intriguing biological properties. this study, we characterized nanovesicles lemon juice (LNVs) evaluated antioxidant effects. We tested LNV activity using human dermal fibroblasts that were pre-treated with LNVs 24 h then stimulated hydrogen peroxide (H
Vascular smooth muscle cells (VSMCs) are key participants in both early- and late-stage atherosclerosis influence neighbouring possibly by means of bioactive molecules, some which packed into extracellular vesicles (EVs). Proprotein convertase subtilisin/kexin type 9 (PCSK9) is expressed secreted VSMCs. This study aimed to unravel the role PCSK9 on VSMCs-derived EVs terms content functionality. were isolated from human VSMCs overexpressing (VSMCPCSK9-EVs) tested endothelial cells, monocytes,...
Multi drug resistant (MDR) bacteria are insensitive to the most common antibiotics currently in use. The spread of antibiotic-resistant bacteria, if not contained, will represent main cause death for humanity 2050. situation is even more worrying when considering patients with chronic bacterial infections, such as those Cystic Fibrosis (CF). development alternative approaches essential and novel therapies that combine exogenous host-mediated antimicrobial action promising. In this work, we...
Abstract Background A few studies suggest that particulate matter (PM) exposure might play a role in bronchiolitis. However, available data are mostly focused on the risk of hospitalization and come from retrospective provided conflicting results. This prospective study investigated association between PM (PM 2.5 10 ) severity Methods cohort was conducted November 2019 February 2020 at pediatric emergency department Fondazione IRCCS Ca′ Ospedale Maggiore Policlinico, Milan, Italy. Infants...
Extracellular vesicles (EVs) are important components of the metastatic niche and crucial in infiltration, metastasis, immune tolerance processes during tumorigenesis. We hypothesized that human endogenous retroviruses (HERV) positive EVs derived from tumor cellsmay have a role modulating innate response. The study was conducted two different colorectal cancer cell lines, representing stages development: Caco-2, non-metastatic adenocarcinoma, SK-CO-1, adenocarcinoma (ascites). Both lines...
ABSTRACT Phage therapy represents a promising strategy for curing bacterial infections refractory to antibiotics. However, the success rate of phage may be lowered by emergence resistance phages used therapy. In this work, we studied CK4 cocktail, which is mixture composed four able cure Pseudomonas aeruginosa in animal models. CK4-resistant mutants were easily isolated from cultures grown either standard laboratory medium or an artificial sputum mimicking composition airway fluid cystic...
Antimicrobial resistance, a major consequence of diagnostic uncertainty and antimicrobial overprescription, is an increasingly recognized cause severe infections, complications, mortality worldwide with huge impact on our society the health system. In particular, patients compromised immune systems or pre-existing chronic pathologies, such as cystic fibrosis (CF), are subjected to frequent antibiotic treatments control infections appearance diffusion multidrug resistant isolates. Therefore,...
Antimicrobial resistance, a major consequence of diagnostic uncertainty and antimicrobial overprescription, is an increasingly recognized cause severe infections, complications, mortality worldwide with huge impact on our society the health system. In particular, patients compromised immune systems or pre-existing chronic pathologies, such as cystic fibrosis (CF), are subjected to frequent antibiotic treatments control infections appearance diffusion multidrug resistant isolates. Therefore,...
Short-term exposure to fine particulate matter (PM2.5) is associated with the activation of adverse inflammatory responses, increasing risk developing acute respiratory diseases, such as those caused by pathogen infections. However, functional mechanisms underlying this evidence remain unclear. In present study, we generated a zebrafish model short-term specific PM2.5, collected in northern metropolitan area Milan, Italy. First, assessed immunomodulatory effects PM2.5 and observed that it...