A5055998079- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Retinal Development and Disorders
- Axon Guidance and Neuronal Signaling
- Advanced biosensing and bioanalysis techniques
- Lipid metabolism and biosynthesis
- Cancer-related molecular mechanisms research
- ATP Synthase and ATPases Research
- Angiogenesis and VEGF in Cancer
- Acute Myeloid Leukemia Research
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Protein Tyrosine Phosphatases
- Autophagy in Disease and Therapy
- Cellular Mechanics and Interactions
- Histone Deacetylase Inhibitors Research
- Microtubule and mitosis dynamics
- Neurogenesis and neuroplasticity mechanisms
- Aquaculture disease management and microbiota
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
IFOM
2009-2021
University of Padua
2002-2010
Center for Genomic Science
2009
Abstract Cystic fibrosis (CF) is a hereditary disease due to mutations in the CFTR gene and causes mortality humans mainly respiratory infections caused by Pseudomonas aeruginosa . In previous work we used phage therapy, which treatment with mix of phages, actively counteract acute P mice Galleria mellonella larvae. this apply therapy PAO1 CF zebrafish model. The structure channel evolutionary conserved between fish mammals cftr -loss-of-function embryos show phenotype that recapitulates...
Cells entering mitosis become rounded, lose attachment to the substrate, and increase their cortical rigidity. Pivotal these events is dismantling of focal adhesions (FAs). How mitotic reshaping linked commitment divide unclear. Here, we show that DEPDC1B, a protein accumulates in G2, coordinates de-adhesion cell-cycle progression at mitosis. DEPDC1B functions as an inhibitor RhoA-based signaling complex, which assembles on FA-associated tyrosine phosphatase, receptor type, F (PTPRF)...
Summary Constitutively active, ‘oncogenic’ H-RAS can drive proliferation and transformation in human cancer, or be a potent inducer of cellular senescence. Moreover, aberrant activation the Ras pathway owing to germline mutations cause severe developmental disorders. In this study we have generated transgenic zebrafish that constitutively express low levels, induced high oncogenic H-RAS. We observed fish carrying integrated transgene their display several hallmarks Costello syndrome, rare...
Notch signaling in prominently involved growth regulation metazoan tissues. Because of this, is often upregulated cancer and current efforts point to developing drugs that block its activation. receptor endocytosis towards acidic compartments a recently appreciated determinant Vacuolar H+ ATPase (V‐ATPase) responsible for acidification endocytic organelles mutants V‐ATPase subunit encoding genes model organisms have been shown display loss signaling. Here, we show administration...
Abstract Vascular lumen formation is a fundamental step during angiogenesis; yet, the molecular mechanisms underlying this process are poorly understood. Recent studies have shown that neural and vascular systems share common anatomical, functional similarities. Here we show organization of endothelial controlled at post-transcriptional level by alternative splicing (AS) regulator Nova2, which was previously considered to be cell-specific. Nova2 expressed angiogenesis its depletion disrupts...
Histone deacetylase 8 (HDAC8), a class I HDAC that modifies non-histone proteins such as p53, is highly expressed in different haematological neoplasms including subtype of acute myeloid leukaemia (AML) bearing inv(16). To investigate HDAC8 contribution to haematopoietic stem cell maintenance and leukaemic transformation, we generated zebrafish model with Hdac8 overexpression observed an increase stem/progenitor cells, phenotype could be reverted using specific inhibitor, PCI-34051 (PCI). In...
In this study we analysed the function of Meinox gene prep1.1 during zebrafish development. proteins form heterotrimeric complexes with Hox and Pbx members, increasing DNA binding specificity in vitro vivo. However, a role for specific protein regulation activity vivo has not been demonstrated. situ hybridization showed that is expressed maternally ubiquitously up to 24 hours post-fertilization (hpf), restricted head from 48 hpf onwards. Morpholino-induced loss-of-function caused significant...
It is unclear whether the establishment of apical-basal cell polarity during generation epithelial lumens requires molecules acting at plasma membrane/actin interface. Here, we show that I-BAR-containing IRSp53 protein controls lumen formation and positioning determinants aPKC podocalyxin. Molecularly, acts by regulating localization activity small GTPase RAB35, interacting with actin capping EPS8. Using correlative light electron microscopy, further ensures shape continuity opposing...
PRDM genes are a family of transcriptional regulators that modulate cellular processes such as differentiation, cell growth and apoptosis. Some members involved in tissue or organ maturation, differentially expressed specific phases embryonic development. PRDM5 is recently identified member functions repressor behaves putative tumor suppressor different types cancer. Using gene expression profiling, we found targets human U2OS cells include critical developmental processes, specifically...
Abstract The Netrin-1 receptor UNC5B is an axon guidance regulator that also expressed in endothelial cells (ECs), where it finely controls developmental and tumor angiogenesis. In the absence of Netrin-1, induces apoptosis blocked upon binding. Here, we identify splicing isoform (called UNC5B-Δ8) exclusively by ECs generated through exon skipping NOVA2, alternative factor regulating vascular development. We show UNC5B-Δ8 a constitutively pro-apoptotic insensitive to required for specific...
The nucleophosmin 1 gene (NPM1) is the most frequently mutated in acute myeloid leukemia. Notably, NPM1 mutations are always accompanied by additional such as those cohesin genes RAD21, SMC1A, SMC3, and STAG2 but not regulator, nipped B-like (NIPBL). In this work, we analyzed a cohort of adult patients with leukemia mutation observed specific reduction expression NIPBL other genes. our zebrafish model, overexpression form also induced downregulation nipblb, ortholog human NIPBL. To...
olig genes encode a previously unrecognized group of vertebrate-specific basic helix-loop-helix transcription factors. As shown in mice, chickens, and zebrafish, two members this group, olig1 olig2, are involved the differentiation motoneurons oligodendrocytes, but nothing is known about role third member, olig3. Here, we show that olig3 plays an essential establishment neural crest-lateral plate boundary. In zebrafish embryos, morpholino-induced inactivation dramatically increases number...
Homozygous mutations in SNAP29, encoding a SNARE protein mainly involved membrane fusion, cause CEDNIK (Cerebral Dysgenesis, Neuropathy, Ichthyosis and Keratoderma), rare congenital neurocutaneous syndrome associated with short life expectancy, whose pathogenesis is unclear. Here, we report the analysis of first genetic model zebrafish. Strikingly, homozygous snap29 mutant larvae display CEDNIK-like features, such as microcephaly skin defects. Consistent Snap29 role fusion during autophagy,...
During embryonic development, new arteries, and veins form from preexisting vessels in response to specific angiogenic signals. Angiogenic signaling is complex since not all endothelial cells exposed signals respond equally. Some will be selected become tip acquire migration proliferation capacity necessary for vessel growth while others, the stalk trailer that stay connected with pre-existing act as a linkage forming vessels. Additionally, have interchange their roles. Stalk cellular...
Abstract Histone deacetylases (HDACs) catalyze the removal of acetyl molecules from histone and non-histone substrates playing important roles in chromatin remodeling control gene expression. Class I HDAC1 is a critical regulator cell cycle progression, cellular proliferation differentiation during development; it also regulated by many post-translational modifications (PTMs). Herein we characterize new mitosis-specific phosphorylation driven Aurora kinases A B. We show that this affects...
// Elisa Barbieri 1, 4 , Gianluca Deflorian 2 Federica Pezzimenti Debora Valli 1 Marco Saia Natalia Meani Alicja M. Gruszka * Myriam Alcalay 3, Department of Experimental Oncology, Istituto Europeo di Oncologia, Milan, Italy The FIRC Institute Molecular Oncology (IFOM) Foundation, 3 Dipartimento Oncologia ed Emato-Oncologia, Università degli Studi Milano, Current address: Gene Expression and Regulation Program, Wistar Institute, Philadelphia, PA, USA These authors contributed equally to this...
Abstract We developed a novel reporter transgenic zebrafish model called MITO-Luc/GFP in which GFP and luciferase expression are under the control of master regulator proliferation NF-Y. In it is possible to visualize cell vivo by fluorescence bioluminescence. this animal model, occur early living embryos, becoming tissue specific juvenile adult zebrafish. By vitro ex experiments we demonstrate that activity animals occurs intestine, kidney gonads, where detectable proliferating cells...