A5056445396- Cellular Mechanics and Interactions
- Protein Tyrosine Phosphatases
- 3D Printing in Biomedical Research
- Galectins and Cancer Biology
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Monoclonal and Polyclonal Antibodies Research
- ATP Synthase and ATPases Research
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Micro and Nano Robotics
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Cancer Research and Treatment
- Advanced Biosensing Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Molecular Communication and Nanonetworks
- Caveolin-1 and cellular processes
- Microfluidic and Bio-sensing Technologies
- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
- DNA Repair Mechanisms
- HIV/AIDS drug development and treatment
- Hippo pathway signaling and YAP/TAZ
- Biological Research and Disease Studies
IFOM
2015-2022
Axxam (Italy)
2020
University of Rome Tor Vergata
2008-2015
Epithelial cells cultured in a monolayer are very motile isolation but reach near-jammed state when mitotic division increases their number above critical threshold. We have recently shown that can be reawakened by over-expression of single protein, RAB5A, master regulator endocytosis. This reawakening motility was explained term flocking transition promotes the emergence large-scale collective migratory pattern. Here we focus on impact this structural properties monolayer. find unjammed is...
There is growing evidence that tyrosine phosphatases display an intrinsic enzymatic preference for the sequence context flanking target phosphotyrosines. On other hand, substrate selection in vivo decisively guided by enzyme-substrate connectivity protein interaction network. We describe here a system wide strategy to infer physiological substrates of protein-tyrosine phosphatases. Here we integrate, Bayesian model, proteome about vitro preference, as determined novel high-density peptide...
Abstract How cells move chemotactically remains a major unmet challenge in cell biology. Emerging evidence indicates that for interpreting noisy, shallow gradients of soluble cues system must behave as an excitable process. Here, through RNAi-based, high-content screening approach, we identify RAB35 necessary the formation growth factors (GFs)-induced waves circular dorsal ruffles (CDRs), apically restricted actin-rich migratory protrusions. is sufficient to induce recurrent and polarized...
Abstract Background The human POB1/REPS2 (Partner of RalBP1) protein is highly conserved in mammals where it has been suggested to function as a molecular scaffold recruiting proteins involved vesicular traffic and linking them the actin cytoskeleton remodeling machinery. More recently was found expressed androgen-dependent prostate cancer cell lines, while one its isoforms (isoform 2) down regulated during progression. Results In this report we characterize central proline rich domain...
The SHP-2 tyrosine phosphatase plays key regulatory roles in the modulation of cell response to growth factors and cytokines. Over past decade, integration genetic, biochemical, structural data has helped interpreting pathological consequences altered function. Using complementary approaches, we provide evidence here that endogenous can dimerize through formation disulfide bonds may also involve catalytic cysteine. We show fraction dimeric is modulated by factor stimulation redox state....
Abstract During wound repair, branching morphogenesis and carcinoma dissemination, cellular rearrangements are fostered by a solid-to-liquid transition known as unjamming. The biomolecular machinery behind unjamming, its physiological clinical relevance remain, however, mystery. Here, we combine biophysical biochemical analysis to study unjamming in variety of epithelial 2D 3D collectives: monolayers, differentiated normal mammary cysts, spheroid models breast ductal situ (DCIS), ex vivo...
Review on REPS2 (RALBP1 associated Eps domain containing 2), with data DNA, the protein encoded, and where gene is implicated.
Multiple transcript variants encoding different isoforms have been found for this gene. Three transcripts are described in Ensembl (ID: ENS0149177). The first (ENS0418331) represents the longer isoform and encodes a protein that consists of an extracellular fibronectin type 3 domain cytosolic catalytic domain. The second (ENS0440289) has multiple differences presence and absence exons at its 3' end, compared to isoform. These produce a unique UTR encoded is shorter consists...