A5068418751- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Micro and Nano Robotics
- Cellular transport and secretion
- Microfluidic and Bio-sensing Technologies
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- Sulfur Compounds in Biology
- interferon and immune responses
- Molecular Communication and Nanonetworks
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Mast cells and histamine
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- NF-κB Signaling Pathways
- Cancer Research and Treatment
- Ecosystem dynamics and resilience
- Mathematical Biology Tumor Growth
- Signaling Pathways in Disease
- Fibroblast Growth Factor Research
- Cell death mechanisms and regulation
- Advanced Fluorescence Microscopy Techniques
- Diffusion and Search Dynamics
IFOM
2013-2023
University of Milan
2016-2019
Ospedale San Paolo
2016-2018
University of Parma
2007-2013
University of Notre Dame
2010
Ospedale di Parma
2007
Ospedale Maggiore
2007
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2007
The mechanisms by which tumor cells metastasize and the role of endocytic proteins in this process are not well understood. We report that overexpression GTPase RAB5A, a master regulator endocytosis, is predictive aggressive behavior metastatic ability human breast cancers. RAB5A necessary sufficient to promote local invasion distant dissemination various mammary nonmammary cell lines, prometastatic associated with increased intratumoral motility. Specifically, for formation invadosomes,...
Abstract The process in which locally confined epithelial malignancies progressively evolve into invasive cancers is often promoted by unjamming, a phase transition from solid-like to liquid-like state, occurs various tissues. Whether this tissue-level mechanical impacts phenotypes during carcinoma progression remains unclear. Here we report that the large fluctuations cell density accompany unjamming result repeated deformations of cells and nuclei. This triggers cellular mechano-protective...
Dense monolayers of living cells display intriguing relaxation dynamics, reminiscent soft and glassy materials close to the jamming transition, migrate collectively when space is available, as in wound healing or cancer invasion. Here we show that collective cell migration occurs bursts are similar those recorded propagation cracks, fluid fronts porous media ferromagnetic domain walls. In analogy with these systems, distribution activity displays scaling laws universal different types for...
Abstract The toxic effects of exogenous hydrogen sulfide on peripheral blood lymphocytes have been investigated in detail. Hydrogen is now considered as a gasotransmitter with specific functional roles different cell types, like neurons and vascular smooth muscle. Here we show that induces caspase‐independent death depends their intracellular glutathion levels, physiologically relevant subset specificity for CD8 + T cells NK cells. Although lymphocyte activation does not modify sensitivity...
Epithelial cells cultured in a monolayer are very motile isolation but reach near-jammed state when mitotic division increases their number above critical threshold. We have recently shown that can be reawakened by over-expression of single protein, RAB5A, master regulator endocytosis. This reawakening motility was explained term flocking transition promotes the emergence large-scale collective migratory pattern. Here we focus on impact this structural properties monolayer. find unjammed is...
A biological tissue is an ensemble of soft cells in close physical contact. Events such as cell-shape changes and, more rarely, cell-divisions and apoptosis continuously occur a tissue, whose collective behavior set by the cumulative occurrence events. In this complex environment, quantifying single-cell dynamics key to extract quantitative information be used capture fundamental ingredients for validating predictions models numerical simulations. However, tracking motion each cell dense...
By combining simulations and experiments, Chepizhko <italic>et al.</italic> show that jammed cellular layers rapidly revert into a flowing state after wound.
Abstract Protein kinase C (PKC)-mediated intracellular signaling participates in several key steps of hematopoietic cell differentiation. The ε isoform PKC has been associated with erythroid differentiation as well the early phases megakaryocytic (MK) lineage commitment. Here, we worked on hypothesis that PKCε expression levels might be modulated during MK differentiation, a specific role late thrombopoiesis. We demonstrate that—at variance development—PKCε is completely downmodulated...
Abstract How cells move chemotactically remains a major unmet challenge in cell biology. Emerging evidence indicates that for interpreting noisy, shallow gradients of soluble cues system must behave as an excitable process. Here, through RNAi-based, high-content screening approach, we identify RAB35 necessary the formation growth factors (GFs)-induced waves circular dorsal ruffles (CDRs), apically restricted actin-rich migratory protrusions. is sufficient to induce recurrent and polarized...
The positive effects of spa therapy on ear, nose, and throat pathology are known but robust literature in this field, is still lacking. aim study was to assess through a retrospective analysis, the otitis media with effusion Politzer endotympanic inhalation sulphurous waters children aged 5-9 years.A cohort 95 patients treated insufflations water: 58 did cycle consisting treatment 12 days per year for three consecutive years; 37 followed same procedure 5 years consecutively. control...
Cell monolayers provide an interesting example of active matter, exhibiting a phase transition from flowing to jammed state as they age. Here we report experiments and numerical simulations illustrating how cellular layer rapidly reverts after wound. Quantitative comparison between shows that cells change their self-propulsion alignement strength so the system crosses line, which characterize by finite-size scaling in particle model. This wound-induced unjamming is found occur generically...
Abstract During wound repair, branching morphogenesis and carcinoma dissemination, cellular rearrangements are fostered by a solid-to-liquid transition known as unjamming. The biomolecular machinery behind unjamming, its physiological clinical relevance remain, however, mystery. Here, we combine biophysical biochemical analysis to study unjamming in variety of epithelial 2D 3D collectives: monolayers, differentiated normal mammary cysts, spheroid models breast ductal situ (DCIS), ex vivo...
Selective evolutionary pressure shapes the processes and genes that enable cancer survival expansion in a tumour-suppressive environment. A distinguishing lethal feature of malignant is its dissemination seeding metastatic foci. key requirement for this process acquisition migratory/invasive ability. However, how migratory phenotype selected during natural evolution what advantage, if any, it might provide to growing cells remain open issues. In opinion piece, we discuss three possible...
Abstract Fragile histidine triad (FHIT) is a tumor suppressor gene whose allelic loss associated to number of human cancers. FHIT protein acts as diadenosine oligophosphate hydrolase, but its suppressive activity appears independent from enzymatic activity. Tumor necrosis factor (TNF)‐related apoptosis inducing ligand (TRAIL) can induce in the FHIT‐negative non‐small lung cancer cell line Calu‐1. We generated four FHIT‐inducible Calu‐1 clones and demonstrated that expression was able protect...
Abstract The process in which locally confined epithelial malignancies progressively evolve into invasive cancers is often promoted by unjamming, a phase transition from solid-like to liquid-like state that occurs various tissues. Whether this tissue-level mechanical impact phenotypes during carcinoma progression remains unclear. We show, here the large fluctuations cell density accompany unjamming result repeated deformations of cells and nuclei. Cells react these protracted stresses...