A5030998224- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Nanomaterials and Printing Technologies
- 3D Printing in Biomedical Research
- Near-Field Optical Microscopy
- Photoacoustic and Ultrasonic Imaging
- Surface Modification and Superhydrophobicity
- Congenital limb and hand anomalies
- Tendon Structure and Treatment
- Microfluidic and Bio-sensing Technologies
- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Biocrusts and Microbial Ecology
- Micro and Nano Robotics
- Photonic Crystals and Applications
- Advanced Materials Characterization Techniques
- RNA Research and Splicing
- Liquid Crystal Research Advancements
- Planarian Biology and Electrostimulation
- Nuclear Structure and Function
- Adhesion, Friction, and Surface Interactions
ETH Zurich
2012-2022
Courant Institute of Mathematical Sciences
2019
New York University
2019
Abstract The mechanical wiring between cells and their surroundings is fundamental to the regulation of complex biological processes during tissue development, repair or pathology. Traction force microscopy (TFM) enables determination actuating forces. Despite progress, important limitations with intrusion effects in low resolution 2D pillar-based methods disruptive intermediate steps cell removal substrate relaxation high-resolution continuum TFM need be overcome. Here we introduce a novel...
Abstract Droplet interactions with compliant materials are familiar, but surprisingly complex processes of importance to the manufacturing, chemical, and garment industries. Despite progress—previous research indicates that mesoscopic substrate deformations can enhance droplet drying or slow down spreading dynamics—our understanding how intertwined effects transient wetting phenomena deformation affect remains incomplete. Here we show above a critical receding contact line speed during...
Abstract Cell polarity refers to the intrinsic asymmetry of cells, including orientation cytoskeleton. It affects cell shape and structure as well distribution proteins organelles. In migratory front-rear is essential dictates movement direction. While link between cytoskeleton nucleus well-studied, we aim investigate if can be transmitted nucleus. We show that knock-down emerin, an integral protein nuclear envelope, abolishes preferential localization several proteins. propose frontally...
Color printing at the diffraction limit has been recently explored by fabricating nanoscale plasmonic structures with electron beam lithography. However, only a limited color range and constant intensity throughout structure have demonstrated. Here we show an alternative, facile approach relying on direct, open-atmosphere electrohydrodynamic rapid NanoDrip of controlled amounts red, green, blue (RGB) quantum dots resolution 250 nm. The narrow emission spectrum allows coverage very broad...
The generation of traction forces and their transmission to the extracellular environment supports disseminative migration cells from a primary tumor. In cancer cells, periodic variation nuclear stiffness during cell cycle provides functional link between efficient translocation proliferation. However, mechanical framework completing this picture remains unexplored. Here, Fucci2 reporter was expressed in various human epithelial resolve phase transition. corresponding tractions were captured...
Topographic modifications of the substrate a cell culture have potential to guide polarization and migration, through which epidermal wound healing may be accelerated. Classic topographic contact guidance is based on interaction between cells supporting scaffold that interferes with establishment focal adhesions, thereby influencing organization actin cytoskeleton. Using soft lithography techniques PDMS, we engineered gratings groove ridge widths 1 μm depth 0.6 μm. These were applied apical...
Traction force microscopy (TFM) derives maps of cell-generated forces, typically in the nanonewton range, transmitted to extracellular environment upon actuation complex biological processes. In traditional approaches, rendering requires a terminal, time-consuming step cell deadhesion obtain reference image. A conceptually opposite approach is provided by reference-free methods, opening on-the-fly generation from an ongoing experiment. This image processing algorithm keeping pace phenomena...
Autologous epidermis grafts generated in vitro represent a promising option for the treatment of burn wounds. The procedure relies on sufficient number cells harvested from healthy tissue, which are then sparsely seeded target surface. time required to reconstitute fully confluent and mature monolayer limited availability cell seeds hinder broad clinical application this procedure. Here, novel engineering approach enhance expansion epithelial tissues is designed experimentally validated....
Abstract Endothelial monolayers physiologically adapt to flow and flow‐induced wall shear stress, attaining ordered configurations in which elongation, orientation, polarization are coherently organized over many cells. Here, with the direction unchanged, a peculiar bi‐stable (along or perpendicular it) cell alignment is observed, emerging as function of intensity alone, while purely instructed by directionality. Driven experimental findings, parallelism between endothelia delineated under...