A5079673853- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Protein Kinase Regulation and GTPase Signaling
- Nuclear Structure and Function
- Retinal Development and Disorders
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Peer-to-Peer Network Technologies
- Caching and Content Delivery
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- RNA Research and Splicing
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Distributed systems and fault tolerance
- Biochemical and Molecular Research
- Microbial infections and disease research
- MicroRNA in disease regulation
- Veterinary Oncology Research
- Physiological and biochemical adaptations
- Hippo pathway signaling and YAP/TAZ
- Multimedia Communication and Technology
- Molecular Sensors and Ion Detection
IFOM
2019-2025
Axxam (Italy)
2024
Istituto Nazionale Genetica Molecolare
2016-2021
University of Bologna
2001-2017
University of Milan
1979-2017
Politecnico di Milano
2008-2013
Toxicologie, Pharmacologie et Signalisation Cellulaire
2013
Norsk Hydro (Norway)
1980
Norwegian Cancer Society
1980
University of Brescia
1979
Abstract Cell polarity refers to the intrinsic asymmetry of cells, including orientation cytoskeleton. It affects cell shape and structure as well distribution proteins organelles. In migratory front-rear is essential dictates movement direction. While link between cytoskeleton nucleus well-studied, we aim investigate if can be transmitted nucleus. We show that knock-down emerin, an integral protein nuclear envelope, abolishes preferential localization several proteins. propose frontally...
Abstract Phosphatidylinositol-5-phosphate (PtdIns5P)−4-kinases (PIP4Ks) are stress-regulated phosphoinositide kinases able to phosphorylate PtdIns5P PtdIns(4,5)P2. In cancer patients their expression is typically associated with bad prognosis. Among the three PIP4K isoforms expressed in mammalian cells, PIP4K2B one more prominent nuclear localisation. Here, we unveil role of as a mechanoresponsive enzyme. protein level strongly decreases cells growing on soft substrates. Its direct silencing...
Significance PIP4Ks are druggable lipid kinases critical in cancer biology whose function human immunity remains unknown. Here, we show that specifically control the growth and activity of a subset immune cells called Tregs, isolated from blood healthy donors. Tregs to exquisitely strength response. If response is too strong, this can trigger autoimmune disease insurgence, while weak responses lead increased infections or enable tumor cell growth. Being able selectively Treg would impact...
Abstract In eukaryotes, cytoplasmic and nuclear volumes are tightly regulated to ensure proper cell homeostasis. However, current methods measure volumes, including confocal 3D reconstruction, have limitations, such as relying on two-dimensional projections or poor vertical resolution. Here, overcome these we describe a method, N2FXm, jointly in single cultured adhering human cells, real time, across cycles. We find that this method accurately provides joint size over dynamic measurements at...
Abstract In the present study we illustrate how, using concept of synthetic lethality (SL), developed cellular assays suitable for screening new drugs targeting cancer-specific weaknesses. SL is a promising strategy in cancer therapy that exploits genetic vulnerabilities cells. This approach targets pairs genes where mutation or changes one gene does not affect cell viability, but simultaneous occurrence these events both leads to death. particularly valuable treatment, as it allows...
Abstract: In rat hippocampal synaptosomes, adenosine decreased the K + (15 m M ) or kainate (1 evoked release of glutamate and aspartate. An even more pronounced effect was observed in presence stable analogue, R ‐phenylisopropyladenosine. All these effects were reversed by selective A 1 receptor antagonist 8‐cyclo‐pentyltheophylline. same synaptosomal preparation, (30 strongly stimulated preloaded [ 3 H]adenosine a partially Ca 2+ ‐dependent tetrodotoxin (TTX)‐sensitive manner. Moreover,...
Abstract: Electrical stimulation of rat hippocampal slices evoked the release excitatory amino acids and purines, as reflected by a time‐dependent increase in extracellular levels glutamate adenosine, well increased efflux radioactivity preloaded with both [ 14 C]glutamate 3 H]adenosine. The purines was amplified when were exposed to 8‐cyclopentyl‐1,3‐dipropylxanthine (a selective A1 adenosine receptor antagonist), (+)‐α‐methyl‐4‐carboxyphenylglycine [a mixed antagonist metabotropic...
Abstract: Presynaptic actions of kainic acid have been tested on uptake and release mechanisms in synaptosome‐enriched preparations from rat hippocampus goldfish brain. Kainic increased a Ca 2+ ‐dependent way the basal endogenous glutamate aspartate both synaptosomal preparations, with maximum effect (40–80%) being reached at highest concentration (1 m M ). In addition, potentiated, an additive or synergic way, excitatory amino acids stimulated by high K + concentrations. 1 showed completely...
Phospholipase C β1 (PLCβ1) is known to play an important role in cell proliferation. Previous studies reported involvement of PLCβ1 G0-G1/S transition and G2/M progression Friend murine erythroleukemia cells (FELC). However, little has been found about its human models. Here, we used K562 line as homologous FELC order investigate the possible key regulatory during proliferation this line. Our on effects overexpression both these isoforms showed a specific positive connection between cyclin...
Phosphatidylinositol (PI) signaling is an essential regulator of cell motility and proliferation. A portion PI metabolism takes place in the nuclear compartment eukaryotic cells, where array kinases phosphatases localize modulate PI. Among these, Diacylglycerol Kinases (DGKs) are a class phosphotransferases that phosphorylate diacylglycerol induce synthesis phosphatidic acid. Nuclear DGKalpha modulates cycle progression, its activity or expression can lead to changes phosphorylated status...
PI-PLCβ1 is involved in cell proliferation, differentiation, and myelodysplastic syndrome (MDS) pathogenesis. Moreover, the increased activity of reduces expression PKC-α, which, turn, delays proliferation linked to erythropoiesis. Lenalidomide currently used low-risk patients with MDS del(5q), where it can suppress del(5q) clone restore normal In this study, we analyzed effect lenalidomide on 16 MDS, as well non-del(5q) hematopoietic lines, mainly focusing erythropoiesis, cycle,...
Through the years, different studies showed involvement of Protein Kinase C (PKC) in cell cycle control, particular during G1/S transition. Little is known about their role at G2/M checkpoint. In this study, using K562 human erythroleukemia line, we found a novel and specific mechanism through which conventional isoform PKCα positively affects Cyclin B1 modulating progression cycle. Since kinase activity PKC was not necessary process, demonstrated that PKCα, physically interacting with B1,...
Abstract Cells and tissue integrity is constantly challenged by the necessity to adapt respond mechanical loads. Among cellular components, nucleus possesses mechano-sensing mechanotransduction capabilities, yet molecular mechanisms involved remain poorly defined. We postulated that properties of chromatin its compartmentalization into condensates contribute nuclear adaptation external forces, while preserving integrity. By interrogating effects MLL4 loss-of-function in Kabuki Syndrome, we...
We examined the expression of metabotropic glutamate (mGlu) receptors in species fish that differ for their vulnerability to anoxic brain damage. Although mGlu1a and mGlu5 was similar all examined, mGlu2/3 substantially higher anoxia-tolerant (i.e., carp Carassius carassius goldfish auratus) than are highly vulnerable damage, such as trouts Salmo trutta Oncorhynchus mykiss. This difference confirmed by measuring receptor-mediated inhibition forskolin-stimulated cAMP formation slices prepared...