A5036094763- Hepatitis C virus research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Hepatitis B Virus Studies
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- PARP inhibition in cancer therapy
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Glycosylation and Glycoproteins Research
- HIV Research and Treatment
- Colorectal Cancer Treatments and Studies
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- Cancer-related molecular mechanisms research
- Diabetes and associated disorders
- Inflammatory Bowel Disease
- Animal Virus Infections Studies
- RNA modifications and cancer
- Liver Diseases and Immunity
- IL-33, ST2, and ILC Pathways
Istituto Nazionale Genetica Molecolare
2015-2024
University of Milan
2015-2024
Ospedale Maggiore
2011-2023
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2011-2023
Ospedale San Giuseppe
2022
MultiMedica
2022
University of Milano-Bicocca
2015-2019
Policlinico Tor Vergata
2015-2017
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2016
Science for Life Laboratory
2015
Chronic hepatitis C virus (HCV) infection occurs in about 3 percent of the world's population and is a major cause liver disease. HCV also associated with cryoglobulinemia, B lymphocyte proliferative disorder. Virus tropism controversial, mechanisms cell entry remain unknown. The envelope protein E2 binds human CD81, tetraspanin expressed on various types including hepatocytes lymphocytes. Binding was mapped to extracellular loop CD81. Recombinant molecules containing this bound antibodies...
We investigated whether human resting T cells could be activated to proliferate and display effector function in the absence of cell receptor occupancy. report that combination interleukin 2 (IL-2), tumor necrosis factor alpha, IL-6 highly purified naive (CD45RA+) memory (CD45RO+) CD4+ proliferate. Under this condition, also as measured by lymphokine synthesis help for immunoglobulin production B cells. This novel Ag-independent pathway activation may play an important role vivo recruiting...
The immune response against hepatitis C virus (HCV) is rarely effective at clearing the virus, resulting in ∼170 million chronic HCV infections worldwide. Here we report that ligation of an receptor (CD81) inhibits natural killer (NK) cells. Cross-linking CD81 by major envelope protein (HCV-E2) or anti-CD81 antibodies blocks NK cell activation, cytokine production, cytotoxic granule release, and proliferation. This inhibitory effect was observed using both activated resting Conversely, on...
The high-throughput analysis of microRNAs (miRNAs) circulating within the blood healthy and diseased individuals is an active area biomarker research. Whereas quantitative real-time reverse transcription polymerase chain reaction (qPCR)-based methods are widely used, it yet unresolved how data should be normalized. Here, we show that a combination different algorithms results in identification candidate reference miRNAs can exploited as normalizers, both discovery validation phases. Using...
Vitamin C promotes anticancer adaptive immunity and enhances efficacy of immune checkpoint therapy.
Characterization of the immune responses to SARS-CoV-2 reveals a CD8 + T cell subset that likely establishes memory virus.
Hepatitis C virus (HCV) is a major cause of chronic hepatitis. The does not replicate efficiently in cell cultures, and it therefore difficult to assess infection-neutralizing antibodies evaluate protective immunity vitro. To study the binding HCV envelope cell-surface receptors, we developed an assay specific recombinant proteins human cells neutralization thereof. expressed various systems were incubated with cells, was assessed by flow cytometry using anti-envelope antibodies. Envelope...
Regulatory T (TR) cells consist of phenotypically and functionally distinct CD4+ CD8+ cell subsets engaged both in maintaining self-tolerance preventing anti–non-self effector responses (microbial, tumor, transplant, so on) that may be harmful to the host. Here we propose proinflammatory function virus-specific memory CCR7–CD8+ cells, which are massively recruited liver, inefficient (in terms IFN-γ production) patients with chronic hepatitis C virus (HCV) infection because concomitant...
Infection with hepatitis C virus (HCV), a leading cause of chronic liver diseases, can associate B lymphocyte proliferative disorders, such as mixed cryoglobulinemia and non-Hodgkin lymphoma. The major envelope protein HCV (HCV-E2) binds, high affinity CD81, tetraspanin expressed on several cell types. Here, we show that engagement CD81 human cells by combination HCV-E2 an anti-CD81 mAb triggers the JNK pathway leads to preferential proliferation naïve (CD27 - ) subset. In parallel, have...
The adult liver is an organ without constitutive lymphoid components. Therefore, any intrahepatic T cell found in chronic hepatitis should have migrated to the after infection and inflammation. Because of little information available on differences between peripheral cells, we used recombinant proteins C virus (HCV) establish specific lines clones from biopsies patients with compared them those present blood mononuclear cells (PBMC). We that protein nonstructural 4 (NS4) was able stimulate...
Invariant natural killer T (NKT) cells are a highly conserved subset of lymphocytes expressing semi-invariant cell receptor (TCR), which is restricted to CD1d and specific for the glycosphingolipid antigen α-galactosylceramide. Their ability secrete variety cytokines, in turn modulate activation both innate acquired immune responses, suggests that invariant NKT exert regulatory role mainly via indirect mechanisms. A relevant question whether can directly help B cells. We document here human...
Invariant natural killer T (iNKT) cells are innate-like lymphocytes recognizing CD1d-restricted glycolipid antigens, such as alpha-galactosylceramide (alphaGC). We assessed whether iNKT help B lymphocyte responses and found that mice immunized with proteins alphaGC develop antibody titers 1-2 logs higher than those induced by alone. Activation of enhances protection against infections influenza elicits frequencies memory to booster immunizations. Protein vaccination alphaGC, but not...
Hepatitis C virus (HCV) is a major human pathogen causing chronic liver disease. We have recently found that the large extracellular loop (LEL) of CD81 binds HCV. This finding prompted us to assess structure-function features HCV-CD81 interaction by using recombinant E2 protein and soluble form LEL. HCV-E2 LEL with K d 1.8 nM; can mediate attachment on hepatocytes; engagement mediates internalization only 30% molecules even after 12 h; four cysteines two disulfide bridges, integrity which...