A5100344574- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Genomic variations and chromosomal abnormalities
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Genomics and Rare Diseases
- Chromosomal and Genetic Variations
- Congenital heart defects research
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- RNA regulation and disease
- Bioinformatics and Genomic Networks
- Animal Genetics and Reproduction
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
Huazhong University of Science and Technology
2012-2025
Zhengzhou University
2021-2025
Southern University of Science and Technology
2016-2024
Huazhong Agricultural University
2024
University of Birmingham
2024
Guilin Medical University
2017-2024
Sun Yat-sen University
2007-2024
Army Medical University
2011-2024
Guangxi University
2024
National Clinical Research Center for Digestive Diseases
2024
microRNAs (miRNAs) are a large class of small non-coding RNAs which post-transcriptionally regulate the expression fraction all animal genes and important in wide range biological processes. Recent advances high-throughput sequencing allow miRNA detection at unprecedented sensitivity, but computational task accurately identifying miRNAs background sequenced remains challenging. For this purpose, we have designed miRDeep2, substantially improved algorithm identifies canonical non-canonical...
As the human life span increases, number of people suffering from cognitive decline is rising dramatically. The mechanisms underlying age-associated memory impairment are, however, not understood. Here we show that disturbances in aging brain mouse are associated with altered hippocampal chromatin plasticity. During learning, aged mice display a specific deregulation histone H4 lysine 12 (H4K12) acetylation and fail to initiate gene expression program consolidation. Restoration physiological...
SummaryData on absolute molecule numbers will empower the modeling, understanding, and comparison of cellular functions biological systems. We quantified transcriptomes proteomes in fission yeast during proliferation quiescence. This rich resource provides first comprehensive reference for all RNA most protein concentrations a eukaryote under two key physiological conditions. The integrated data set supports quantitative biology affords unique insights into cell regulation. Although mRNAs...
Copy number variations (CNVs) account for a substantial proportion of human genomic variation, and have been shown to cause neurodevelopmental disorders.We sought determine the relevance CNVs aetiology schizophrenia (SZ).Whole-genome, high-resolution, tiling path BAC array comparative hybridization (array CGH) was employed test DNA from 93 individuals with DSM-IV SZ.Common copy changes that are unlikely be directly pathogenic in SZ were filtered out by comparison reference dataset 372...
We have developed a transcriptome-wide approach to identify genes affected by promoter CpG island DNA hypermethylation and transcriptional silencing in colorectal cancer. By screening cell lines validating tumor-specific panel of primary human cancer samples, we estimate that nearly 5% or more all known may be methylated an individual tumor. When directly compared gene mutations, find larger numbers hypermethylated tumors, higher frequency within harboring either genetic epigenetic changes....
Do young and old protein molecules have the same probability to be degraded? We addressed this question using metabolic pulse-chase labeling quantitative mass spectrometry obtain degradation profiles for thousands of proteins. find that >10% proteins are degraded non-exponentially. Specifically, less stable in first few hours their life stabilize with age. Degradation conserved similar two cell types. Many non-exponentially (NED) subunits complexes produced super-stoichiometric amounts...
Changes in gene expression levels determine differentiation of tissues involved development and are associated with functional decline aging. Although is tightly regulated, the transition between aging, as well regulation post-developmental changes, not understood. Here, we measured messenger RNA (mRNA), microRNA (miRNA), protein prefrontal cortex humans rhesus macaques over species' life spans. We find that few changes unique to Instead, vast majority miRNA occur aging represent reversals...
Abstract Background Microarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies. Although free of many limitations imposed microarray design, its potential to transcript levels unknown. Results In this study, we evaluate relative accuracy microarrays and (RNA-Seq)...
Cellular metabolism was recently shown to regulate endothelial cell phenotype profoundly. Whether the atheroprotective biomechanical stimulus elicited by laminar shear stress modulates is not known.Here, we show that flow exposure reduced glucose uptake and mitochondrial content in endothelium. Shear stress-mediated reduction of reversed silencing flow-sensitive transcription factor Krüppel-like 2 (KLF2). Endothelial-specific deletion KLF2 mice induced cells perfused hearts. overexpression...
Over the course of ontogenesis, human brain and cognitive abilities develop in parallel, resulting a phenotype strikingly distinct from that other primates. Here, we used microarrays RNA-sequencing to examine human-specific gene expression changes taking place during postnatal development prefrontal cortex cerebellum humans, chimpanzees, rhesus macaques. We show most prominent change affects genes associated with synaptic functions represents an extreme shift timing cortex, but not...
While splicing differences between tissues, sexes and species are well documented, little is known about the extent nature of changes that take place during human or mammalian development aging. Here, using high‐throughput transcriptome sequencing, we have characterized whole lifespan in two brain regions: prefrontal cortex cerebellum. Identified were confirmed independent rhesus macaque RNA‐seq data sets, exon arrays PCR, detected at protein level mass spectrometry. Splicing across abundant...
Abstract Current treatments for clear cell renal cancer (ccRCC) are insufficient because two-thirds of patients with metastases progress within two years. Here we report the identification and characterization a stem (CSC) population in ccRCC. CSCs quantitatively correlated tumor aggressiveness metastasis. Transcriptional profiling single sequencing reveal that these exhibit an activation WNT NOTCH signaling. A significant obstacle to development rational has been discrepancy between model...