A5101970478- Forest Insect Ecology and Management
- Plant Pathogens and Fungal Diseases
- Botany and Plant Ecology Studies
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Plant Pathogenic Bacteria Studies
- Adipose Tissue and Metabolism
- Digital Media and Visual Art
- Plant and animal studies
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Plant Molecular Biology Research
- Animal Behavior and Reproduction
- Insect and Arachnid Ecology and Behavior
- Genetic Mapping and Diversity in Plants and Animals
- Antimicrobial Peptides and Activities
Northwestern Polytechnical University
2017-2024
Beijing VDJBio (China)
2024
Henan University
2023
Beijing Polytechnic
2023
Tianjin Medical University
2023
Shanghai Jiao Tong University
2022
Fujian Agriculture and Forestry University
2011-2022
Shanghai First People's Hospital
2022
University of Lausanne
2014-2020
Institute of Genetics and Developmental Biology
2010-2019
In development, timing is of the utmost importance, and developmental processes often changes as organisms evolve. human evolution, retardation, or neoteny, has been proposed a possible mechanism that contributed to rise many human-specific features, including an increase in brain size emergence cognitive traits. We analyzed mRNA expression prefrontal cortex humans, chimpanzees, rhesus macaques determine whether neotenic are present at gene level. show transcriptome dramatically remodeled...
Changes in gene expression levels determine differentiation of tissues involved development and are associated with functional decline aging. Although is tightly regulated, the transition between aging, as well regulation post-developmental changes, not understood. Here, we measured messenger RNA (mRNA), microRNA (miRNA), protein prefrontal cortex humans rhesus macaques over species' life spans. We find that few changes unique to Instead, vast majority miRNA occur aging represent reversals...
Over the course of ontogenesis, human brain and cognitive abilities develop in parallel, resulting a phenotype strikingly distinct from that other primates. Here, we used microarrays RNA-sequencing to examine human-specific gene expression changes taking place during postnatal development prefrontal cortex cerebellum humans, chimpanzees, rhesus macaques. We show most prominent change affects genes associated with synaptic functions represents an extreme shift timing cortex, but not...
While splicing differences between tissues, sexes and species are well documented, little is known about the extent nature of changes that take place during human or mammalian development aging. Here, using high‐throughput transcriptome sequencing, we have characterized whole lifespan in two brain regions: prefrontal cortex cerebellum. Identified were confirmed independent rhesus macaque RNA‐seq data sets, exon arrays PCR, detected at protein level mass spectrometry. Splicing across abundant...
While multiple studies have reported the accelerated evolution of brain gene expression in human lineage, mechanisms underlying such changes are unknown. Here, we address this issue from a developmental perspective, by analyzing mRNA and microRNA (miRNA) two regions within macaques, chimpanzees, humans throughout their lifespan. We find that constitutive divergence (species differences independent age) is comparable between chimpanzees. However, display 3-5 times faster evolutionary rate...
Piwi-interacting RNA (piRNA) are small abundant in the germline across animal species. In fruit flies and mice, piRNA have been implicated maintenance of genomic integrity by transposable elements silencing. Outside germline, only found fly ovarian follicle cells. Previous studies further reported presence multiple piRNA-like (pilRNA) heads a number pilRNA mouse tissues human NK Here, we analyze high-throughput sequencing data more than 130 fly, rhesus macaque samples. The results show...
Abstract Background During microRNA (miRNA) maturation in humans and flies, Drosha Dicer cut the precursor transcript, thereby producing a short RNA duplex. One strand of this duplex becomes functional component RNA-Induced Silencing Complex (RISC), while other is eliminated. While thermodynamic asymmetry ends appears to play decisive role selection process, details mechanism are not yet understood. Results Here, we assess miRNA bias fruit flies by analyzing sequence composition relative...
Lipids are prominent components of the nervous system. Here we performed a large-scale mass spectrometry-based analysis lipid composition three brain regions as well kidney and skeletal muscle humans, chimpanzees, rhesus macaques, mice. The human shows most distinct composition: 76% 5,713 compounds examined in our study either enriched or depleted brain. Concentration levels lipids evolve approximately four times faster among primates compared with characteristic non-neural tissues show...
Among other factors, changes in gene expression on the human evolutionary lineage have been suggested to play an important role establishment of human-specific phenotypes. However, molecular mechanisms underlying these are largely unknown. Here, we explored microRNA (miRNA) regulation divergence among adult humans, chimpanzees, and rhesus macaques, two brain regions: prefrontal cortex cerebellum. Using a combination high-throughput sequencing, miRNA microarrays, Q-PCR, shown that up 11% 325...
MicroRNA-mediated gene regulation is important in many physiological processes. Here we explore the roles of a microRNA, miR-941, human evolution. We find that miR-941 emerged de novo lineage, between six and one million years ago, from an evolutionarily volatile tandem repeat sequence. Its copy-number remains polymorphic humans shows trend for decreasing with migration out Africa. Emergence was accompanied by accelerated loss miR-941-binding sites, presumably to escape regulation. further...
Human evolution is characterized by the rapid expansion of brain size and drastic increase in cognitive capabilities. It has long been suggested that these changes were accompanied modifications metabolism. Indeed, human-specific on gene expression or amino acid sequence reported for a number metabolic genes, but actual metabolite measurements humans apes have remained scarce. Here, we investigate concentrations more than 100 metabolites prefrontal cerebellar cortex 49 humans, 11...
Although Neanderthals are extinct, fragments of their genomes persist in contemporary humans. Here we show that while the genome-wide frequency Neanderthal-like sites is approximately constant across all out-of-Africa populations, genes involved lipid catabolism contain more than threefold excess such humans European descent. Evolutionally, these significant association with signatures recent positive selection European, but not Asian or African populations. Functionally, can be linked a...
Metabolite concentrations reflect the physiological states of tissues and cells. However, role metabolic changes in species evolution is currently unknown. Here, we present a study metabolome conducted three brain regions two non-neural from humans, chimpanzees, macaque monkeys, mice based on over 10,000 hydrophilic compounds. While chimpanzee, macaque, mouse metabolomes diverge following genetic distances among species, detect remarkable acceleration human prefrontal cortex skeletal muscle...
Transcription is the first step connecting genetic information with an organism's phenotype. While expression of annotated genes in human brain has been characterized extensively, our knowledge about scope and conservation transcripts located outside known genes' boundaries limited. Here, we use high-throughput transcriptome sequencing (RNA-Seq) to characterize total non-ribosomal human, chimpanzee, rhesus macaque brain. In all species, only 20–28% correspond exons 20–23% introns. By...
Epigenetic modifications are critical determinants of cellular and developmental states. changes, such as decreased H3K27me3 histone methylation on insulin/IGF1 genes, have been previously shown to modulate lifespan through gene expression regulation. However, global epigenetic changes during aging their biological functions, if any, remain elusive. Here, we examined the modification H3K4 dimethylation (H3K4me2) in prefrontal cortex individual rhesus macaques at different ages by chromatin...
Miniature inverted-repeat transposable element (MITE) is a type of class II non-autonomous playing crucial role in the process evolution biology. There an urgent need to develop bioinformatics tools effectively identify MITEs on whole genome-wide scale. However, most currently existing suffer from low ability deal with large eukaryotic genomes. In this paper, we proposed novel tool MiteFinderII, which was adapted our previous algorithm MiteFinder, efficiently detect genomics sequences. It...
Abstract OsWUS has recently been shown to be a transcription factor gene critical for tiller development and fertility in rice. The protein consists of three conserved structural domains, but their biological functions are still unclear. We discovered new rice mutant resulting from tissue culture, which hardly produced tillers exhibited complete female sterility. male floral organs the were morphologically indistinguishable those wild type. named srt1 completely sterile reduced tillering 1....
Adenosine-to-inosine (A-to-I) substitutions are the most common type of RNA editing in mammals. A-to-I is particularly widespread brain and known to play important roles neuronal functions. In this study we investigated RNA-editing changes during human development maturation, as well evolutionary conservation patterns across primates. We used high-throughput transcriptome sequencing (RNA-seq) quantify levels assess ontogenetic dynamics at more than 8000 previously annotated exonic sites two...
Sporopollenin in the pollen cell wall protects male gametophytes from stresses. Phenylpropanoid derivatives, including guaiacyl (G) lignin units, are known to be structural components of sporopollenin, but exact composition sporopollenin remains fully resolved. We analyzed phenylpropanoid derivatives maize and Arabidopsis by thioacidolysis coupled with nuclear magnetic resonance (NMR) gas chromatography-mass spectrometry (GC-MS). The NMR GC-MS results confirmed presence p-hydroxyphenyl (H),...
Small nuclear and nucleolar RNAs (snRNAs snoRNAs) are known to be functionally evolutionarily conserved elements of transcript processing machinery. Here, we investigated the expression evolution snRNAs snoRNAs by measuring their abundance in frontal cortex humans, chimpanzees, rhesus monkeys, mice. Although snRNA is largely conserved, 44% 185 measured snoRNA 40% 134 families showed significant divergence among species. The included drastic changes unique humans: A 10-fold elevated U1 a...
High-density genetic maps can significantly improve the resolution of QTL mapping. We constructed a high-density recombination bin-based map eggplant based on 200 F2 plants from an interspecific cross (Solanum melongena × S. incanum) using whole genome resequencing strategy. The was 2022.8 cM long, covering near 99% genome. contained 3776 bins, with 3644 (96.5%) being effective (position non-redundant) ones, giving nominal average distance 0.54 and 0.56 between adjacent respectively. Using...