A5016730202- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Metabolism and Genetic Disorders
- Cancer-related Molecular Pathways
- Nutrition, Genetics, and Disease
- Single-cell and spatial transcriptomics
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Mitochondrial Function and Pathology
- Molecular Biology Techniques and Applications
- Esophageal and GI Pathology
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Alzheimer's disease research and treatments
- Cancer-related molecular mechanisms research
- Ginger and Zingiberaceae research
- Genomics and Chromatin Dynamics
Jinggangshan University
2010-2025
Xiangya Hospital Central South University
2025
Central South University
2025
Harbin Institute of Technology
2023-2024
Southern University of Science and Technology
2018-2024
XinHua Hospital
2008-2024
The University of Texas Southwestern Medical Center
2020-2024
Weatherford College
2024
Meizhou City People's Hospital
2024
Chongqing Medical University
2022-2024
Changes in gene expression levels determine differentiation of tissues involved development and are associated with functional decline aging. Although is tightly regulated, the transition between aging, as well regulation post-developmental changes, not understood. Here, we measured messenger RNA (mRNA), microRNA (miRNA), protein prefrontal cortex humans rhesus macaques over species' life spans. We find that few changes unique to Instead, vast majority miRNA occur aging represent reversals...
Over the course of ontogenesis, human brain and cognitive abilities develop in parallel, resulting a phenotype strikingly distinct from that other primates. Here, we used microarrays RNA-sequencing to examine human-specific gene expression changes taking place during postnatal development prefrontal cortex cerebellum humans, chimpanzees, rhesus macaques. We show most prominent change affects genes associated with synaptic functions represents an extreme shift timing cortex, but not...
While splicing differences between tissues, sexes and species are well documented, little is known about the extent nature of changes that take place during human or mammalian development aging. Here, using high‐throughput transcriptome sequencing, we have characterized whole lifespan in two brain regions: prefrontal cortex cerebellum. Identified were confirmed independent rhesus macaque RNA‐seq data sets, exon arrays PCR, detected at protein level mass spectrometry. Splicing across abundant...
Drosophila has long been a successful model organism in multiple biomedical fields. Spatial gene expression patterns are critical for the understanding of complex pathways and interactions, whereas temporal changes vital studying highly dynamic physiological activities. Systematic studies still impeded by lack spatiotemporal transcriptomic information. Here, utilizing spatial enhanced resolution omics-sequencing (Stereo-seq), we dissected developing with high sensitivity. We demonstrated...
While multiple studies have reported the accelerated evolution of brain gene expression in human lineage, mechanisms underlying such changes are unknown. Here, we address this issue from a developmental perspective, by analyzing mRNA and microRNA (miRNA) two regions within macaques, chimpanzees, humans throughout their lifespan. We find that constitutive divergence (species differences independent age) is comparable between chimpanzees. However, display 3-5 times faster evolutionary rate...
Piwi-interacting RNA (piRNA) are small abundant in the germline across animal species. In fruit flies and mice, piRNA have been implicated maintenance of genomic integrity by transposable elements silencing. Outside germline, only found fly ovarian follicle cells. Previous studies further reported presence multiple piRNA-like (pilRNA) heads a number pilRNA mouse tissues human NK Here, we analyze high-throughput sequencing data more than 130 fly, rhesus macaque samples. The results show...
RBM10 encodes an RNA binding protein. Mutations in are known to cause multiple congenital anomaly syndrome male humans, the TARP syndrome. However, molecular function of is unknown. Here we used PAR‐CLIP identify thousands sites and observed significant RBM10–RNA interactions vicinity splice sites. Computational analyses as well loss‐of‐function gain‐of‐function experiments provided evidence for regulating exon skipping suggested underlying mechanistic model, which could be subsequently...
Innate lymphoid cells (ILC) are part of a heterogeneous family haematopoietic effector which lack re-arranged antigen-specific receptors. They promote host defense and contribute to tissue metabolic homeostasis, wound healing immune surveillance. Their role in human cancer immunity is less defined, therefore we aimed identify the frequency phenotype distinct ILC groups various types cancer.Tissue samples peripheral blood were collected from patients undergoing surgical resection...
Blood-brain barrier (BBB) characteristics are induced and maintained by crosstalk between brain microvascular endothelial cells neighboring cells. Using in vitro cell models, we previously found that a bystander effect was cause for Japanese encephalitis-associated disruption. Brain astrocytes, which neighbor BBB cells, play roles the maintenance of integrity. By extending scope relevant studies, potential mechanism has been shown activation astrocytes could be disruption integrity during...
Abstract Human cancer cell lines have long served as tools for research and drug discovery, but the presence source of intra-cell-line heterogeneity remain elusive. Here, we perform single-cell RNA-sequencing ATAC-sequencing on 42 39 human lines, respectively, to illustrate both transcriptomic epigenetic within individual lines. Our data reveal that is frequently observed in different tissue origins, often driven by multiple common transcriptional programs. Copy number variation, well...
Spastic paraplegia 50 (SPG50) is an ultrarare childhood-onset neurological disorder caused by biallelic loss-of-function variants in the AP4M1 gene. SPG50 characterized progressive spastic paraplegia, global developmental delay, and subsequent intellectual disability, secondary microcephaly, epilepsy. We preformed preclinical studies evaluating adeno-associated virus (AAV)/AP4M1 gene therapy for describe vitro that demonstrate transduction of patient-derived fibroblasts with AAV2/AP4M1,...
Genomic and transcriptomic alterations affecting key cellular processes such us cell proliferation, differentiation genomic stability are considered crucial for the development progression of cancer. Most invasive breast carcinomas known to derive from precursor in situ lesions. It is proposed that major global expression abnormalities occur transition normal premalignant stages further stages. Serial analysis gene (SAGE) was employed generate a comprehensive profile changes occurring during...
Abstract Background Estrogens are known to regulate the proliferation of breast cancer cells and modify their phenotypic properties. Identification estrogen-regulated genes in human tumors is an essential step toward understanding molecular mechanisms estrogen action cancer. To this end we generated compared Serial Analysis Gene Expression (SAGE) profiles 26 carcinomas based on receptor α (ER) status. Thus, producing a SAGE database almost 2.5 million tags, representing over 50,000...
Abstract Global gene expression measured by DNA microarray platforms have been extensively used to classify breast carcinomas correlating with clinical characteristics, including outcome. We generated a cancer Serial Analysis of Gene Expression (SAGE) high-resolution database ∼2.7 million tags perform unsupervised statistical analyses obtain the molecular classification breast-invasive ductal in correlation clinicopathologic features. Unsupervised analysis means random forest approach...
As a major cellular defence mechanism, the Nrf2/Keap1 pathway regulates expression of genes involved in detoxification and stress response. Here we hypothesise that Nrf2 is oesophageal barrier function plays protective role against gastro-oesophageal reflux disease (GERD).Human biopsy samples, mouse surgical models Nrf2(-/-) mice were used to assess function. Trans-epithelial electrical resistance (TEER) was measured with mini-Ussing chambers. HE staining transmission electron microscopy...
Ginger has received extensive attention because of its antioxidant, anti-inflammatory, and antitumor activities. However, the metabolic fate major components is still unclear. In present study, metabolism [6]-shogaol, one active in ginger, was examined for first time mice cancer cells. Thirteen metabolites were detected identified, seven which purified from fecal samples collected [6]-shogaol-treated mice. Their structures elucidated as 1-(4'-hydroxy-3'-methoxyphenyl)-4-decen-3-ol (M6),...
Neuronal ceroid lipofuscinosis type 7 (CLN7) disease is a lysosomal storage caused by mutations in the facilitator superfamily domain containing 8 (MFSD8) gene, which encodes membrane-bound protein, MFSD8. To test effectiveness and safety of adeno-associated viral (AAV) gene therapy, an vitro study demonstrated that AAV2/MFSD8 dose dependently rescued function fibroblasts from CLN7 patient. An vivo efficacy using intrathecal administration AAV9/MFSD8 to Mfsd8– /– mice at P7–P10 or P120 with...
Cells do not live in a vacuum, but milieu defined by cell–cell communication that can be measured via emerging high-resolution spatial transcriptomics approaches. However, analytical tools fully leverage such data for kinetic modeling remain lacking. Here we present Spateo ( aristoteleo/spateo-release ), general framework quantitative spatiotemporal of single-cell resolution transcriptomics. delivers novel methods digitizing layers/columns to identify spatially-polar genes, and develops...
Abstract Epithelial–mesenchymal transition (EMT) is a fundamental cellular process frequently hijacked by cancer cells to promote tumor progression, especially metastasis. EMT orchestrated complex molecular network acting at different layers of gene regulation. In addition transcriptional regulation, posttranscriptional mechanisms may also play role in EMT. Here, we performed pooled CRISPR screen analyzing the influence 1,547 RNA-binding proteins on cell motility colon and identified...
Mechanisms controlling human multipotent mesenchymal (stromal) stem cell (hMSC) differentiation into osteoblasts or adipocytes are poorly understood. We have previously demonstrated that Wnt signaling in hMSC enhanced osteoblast and inhibited adipogenesis by comparing two lines overexpressing mutated forms of the co-receptor LRP5: T253I (hMSC-LRP5(T253)) T244M (hMSC-LRP5(T244)) conducting high low level signaling, respectively. To explore underlying molecular mechanisms, we compared gene...
In this study, we identified Nrf2 as a molecular target of [6]-shogaol (6S), bioactive compound isolated from ginger, in colon epithelial cells vitro and vivo. Following 6S treatment HCT-116 cells, the intracellular GSH/GSSG ratio was initially diminished but then elevated above basal level. Intracellular reactive oxygen species (ROS) correlated inversely with ratio. Further analysis using gene microarray showed that upregulated expression genes (AKR1B10, FTL, GGTLA4, HMOX1) cells. Western...
MicroRNAs (miRNAs) constitute an important class of small regulatory RNAs that are derived from distinct hairpin precursors (pre-miRNAs). In contrast to mature miRNAs, which have been characterized in numerous genome-wide studies different organisms, research on global profiling pre-miRNAs is limited. Here, using massive parallel sequencing, we performed characterization both mouse and precursor miRNAs. total, 87 369 704 252 003 sequencing reads 887 281 miRNAs were obtained, respectively....
Abstract Gene annotation is a critical resource in genomics research. Many computational approaches have been developed to assemble transcriptomes based on high-throughput short-read sequencing, however, only with limited accuracy. Here, we combine next-generation and third-generation sequencing reconstruct full-length transcriptome the rat hippocampus, which further validated using independent 5´ 3´-end profiling approaches. In total, detect 28,268 transcripts (FLTs), covering 6,380 RefSeq...