A5100420891- Single-cell and spatial transcriptomics
- Coccidia and coccidiosis research
- RNA Interference and Gene Delivery
- Helminth infection and control
- Genomics and Chromatin Dynamics
- Protein Hydrolysis and Bioactive Peptides
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Insect and Arachnid Ecology and Behavior
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Wound Healing and Treatments
- Genomics and Phylogenetic Studies
- Inhalation and Respiratory Drug Delivery
- Tendon Structure and Treatment
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- Market Dynamics and Volatility
- Phytochemicals and Antioxidant Activities
- Microbial Community Ecology and Physiology
- Remote Sensing and Land Use
- Cellular Mechanics and Interactions
- Energy, Environment, and Transportation Policies
- Cell Image Analysis Techniques
- Plant-Microbe Interactions and Immunity
Southeast University
2022-2025
Meso Scale Discovery (United States)
2022-2025
First Affiliated Hospital of Zhengzhou University
2016-2025
Nanjing Agricultural University
2022-2025
Advanced Materials and Devices (United States)
2025
Donghua University
2024
Qingdao University
2022-2024
Xiamen University
2024
Wuhan University
2015-2024
State Key Laboratory of Hybrid Rice
2024
Integrative analysis of multi-omics layers at single cell level is critical for accurate dissection cell-to-cell variation within certain populations. Here we report scCAT-seq, a technique simultaneously assaying chromatin accessibility and the transcriptome same cell. We show that combined signatures enable construction regulatory relationships between cis-regulatory elements target genes single-cell resolution, providing new dimension features helps direct discovery patterns specific to...
Drosophila has long been a successful model organism in multiple biomedical fields. Spatial gene expression patterns are critical for the understanding of complex pathways and interactions, whereas temporal changes vital studying highly dynamic physiological activities. Systematic studies still impeded by lack spatiotemporal transcriptomic information. Here, utilizing spatial enhanced resolution omics-sequencing (Stereo-seq), we dissected developing with high sensitivity. We demonstrated...
Human X-box binding protein-1 (XBP1) is an alternatively spliced transcription factor that participates in the unfolded protein response (UPR), a stress-signaling pathway allows cells to survive accumulation of proteins endoplasmic reticulum lumen. We have previously demonstrated XBP1 expression increased antiestrogen-resistant breast cancer cell lines and coexpressed with estrogen receptor alpha (ER) tumors. The purpose this study investigate role UPR antiestrogen responsiveness cancer....
Human pre-implantation embryonic development involves extensive changes in chromatin structure and transcriptional activity. Here, we report on LiCAT-seq, a technique that enables simultaneous profiling of accessibility gene expression with ultra-low input cells, map the transcriptome landscapes for human embryos. We observed global difference between sperm all stages embryos, finding accessible regions tend to occur gene-poor genomic regions. Integrative analyses two datasets reveals strong...
The Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) is a fundamental epigenomics approach and has been widely used in profiling the chromatin accessibility dynamics multiple species. A comprehensive reference of ATAC-seq datasets mammalian tissues important understanding regulatory specificity developmental abnormality caused by genetic or environmental alterations. Here, we report an adult mouse atlas producing total 66 profiles from 20 primary both male female mice....
Osteochondral defects (OCD) cannot be efficiently repaired due to the unique physical architecture and pathological microenvironment including enhanced oxidative stress inflammation. Conventional strategies, such as control of implant microstructure or introduction growth factors, have limited functions failing manage these complex environments. Here we developed a multifunctional silk-based hydrogel incorporated with metal-organic framework nanozymes (CuTA@SF) provide suitable for OCD...
Dry eye is a common ocular disease that results in discomfort and impaired vision, impacting an individual's quality of life. A great number drugs administered drops to treat dry are poorly soluble water rapidly eliminated from the surface, which limits their therapeutic effects. Therefore, it imperative design novel drug delivery system not only improves solubility but also prolongs its retention time on surface. Herein, we develop copolymer mono-functional POSS, PEG, PPG (MPOSS-PEG-PPG,...
High levels of mitochondrial reactive oxygen species (mROS) are linked to cancer development, which is tightly controlled by the electron transport chain (ETC). However, epigenetic mechanisms governing ETC gene transcription drive mROS production and cell growth remain be fully characterized. Here, we report that protein demethylase PHF8 overexpressed in many types cancers, including colon lung cancer, negatively correlated with expression. While it well known demethylate histones activate...
Abstract Single-cell technologies are becoming increasingly widespread and have been revolutionizing our understanding of cell identity, state, diversity function. However, current platforms can be slow to apply large-scale studies resource-limited clinical arenas due a variety reasons including cost, infrastructure, sample quality requirements. Here we report DNBelab C4 (C4), negative pressure orchestrated, portable cost-effective device that enables high-throughput single-cell...
The black rockfish (Sebastes schlegelii) is a teleost in which eggs are fertilized internally and retained the maternal reproductive system, where they undergo development until live birth (viviparity). In present study, we report chromosome-level genome assembly. High-throughput transcriptome analysis (RNA-seq ATAC-seq) coupled with situ hybridization (ISH) immunofluorescence reveal several candidate genes for preparation, sperm storage release, hatching. We propose that zona pellucida (ZP)...
Abstract Ant colonies with permanent division of labour between castes and highly distinct roles the sexes have been conceptualized to be superorganisms, but cellular molecular mechanisms that mediate caste/sex-specific behavioural specialization remained obscure. Here we characterized brain cell repertoire queens, gynes (virgin queens), workers males Monomorium pharaonis by obtaining 206,367 single-nucleus transcriptomes. In contrast Drosophila , mushroom body Kenyon cells are abundant in...
Cells do not live in a vacuum, but milieu defined by cell–cell communication that can be measured via emerging high-resolution spatial transcriptomics approaches. However, analytical tools fully leverage such data for kinetic modeling remain lacking. Here we present Spateo ( aristoteleo/spateo-release ), general framework quantitative spatiotemporal of single-cell resolution transcriptomics. delivers novel methods digitizing layers/columns to identify spatially-polar genes, and develops...
Abstract Single cell approaches have increased our knowledge about the type composition of non-human primate (NHP), but a detailed characterization area-specific regulatory features remains outstanding. We generated single-cell transcriptomic and chromatin accessibility (single-cell ATAC) data 358,237 cells from prefrontal cortex (PFC), primary motor (M1) visual (V1) adult female cynomolgus monkey brain, integrated this dataset with Stereo-seq (spatial enhanced resolution omics-sequencing)...
Abstract Stiffness is an important physical property of biomaterials that determines stem cell fate. Guiding differentiation via stiffness modulation has been considered in tissue engineering. However, the mechanism by which material regulates into tendon lineage remains controversial. Increasing evidence demonstrates immune cells interact with implanted and regulate behaviors paracrine signaling; however, role this not clear. In study, polydimethylsiloxane (PDMS) substrates different...