A5073481164- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Endoplasmic Reticulum Stress and Disease
- Immune cells in cancer
- Chronic Kidney Disease and Diabetes
- Cell Image Analysis Techniques
- Sphingolipid Metabolism and Signaling
- Cancer Immunotherapy and Biomarkers
- Diet, Metabolism, and Disease
- Blood Pressure and Hypertension Studies
- Reproductive Biology and Fertility
- Mosquito-borne diseases and control
- Prenatal Screening and Diagnostics
- Plant Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Ovarian function and disorders
- Renal and related cancers
- Pancreatic function and diabetes
- Plant-Microbe Interactions and Immunity
- Geomechanics and Mining Engineering
- Sensorless Control of Electric Motors
- Genomics and Chromatin Dynamics
- Health Promotion and Cardiovascular Prevention
- Advanced MEMS and NEMS Technologies
Nanjing Agricultural University
2024-2025
Sichuan University of Science and Engineering
2025
Fujian University of Traditional Chinese Medicine
2024
Chongqing University
2024
Shijiazhuang Tiedao University
2024
Air Force Medical University
2023
Sun Yat-sen University
2023
Southern Medical University
2018-2022
Nanfang Hospital
2020-2022
Shenyang Pharmaceutical University
2022
Integrative analysis of multi-omics layers at single cell level is critical for accurate dissection cell-to-cell variation within certain populations. Here we report scCAT-seq, a technique simultaneously assaying chromatin accessibility and the transcriptome same cell. We show that combined signatures enable construction regulatory relationships between cis-regulatory elements target genes single-cell resolution, providing new dimension features helps direct discovery patterns specific to...
Thrombotic events cannot be completely prevented by antithrombotics, implicating a therapeutic gap due to inflammation, not yet sufficiently addressed mechanism. Neutrophil extracellular traps (NETs) are an essential interface between inflammation and thrombosis, but exactly how the NETotic process is initiated maintained during arterial thrombosis remains incompletely understood.We found that plasma concentrations of C5a were higher in patients with ST-elevation myocardial infarction...
Viral infection causes multiple forms of human cancer, and HPV is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight tumor heterogeneity present most cancers, but virally induced tumors have not been studied. HeLa a well characterized HPV+ cancer cell line. We developed new high throughput platform to prepare RNA on nanoliter scale based customized microwell chip. Using this method, we successfully amplified full-length transcripts 669 single S3 cells 40...
Medical artificial intelligence (AI) has been moving from the research phase to clinical implementation. However, most AI-based models are mainly built using high-quality images preprocessed in laboratory, which is not representative of real-world settings. This dataset bias proves a major driver AI system dysfunction. Inspired by design flow cytometry, DeepFundus, deep-learning-based fundus image classifier, developed provide automated and multidimensional sorting address this data quality...
Abstract The liver and gallbladder are among the most important internal organs derived from endoderm, yet development of in early embryonic stages is not fully understood. Using a transgenic Foxa2 eGFP reporter mouse line, we performed single-cell full-length mRNA sequencing on endodermal hepatic cells isolated ten stages, ranging E7.5 to E15.5. We identified developmental trajectory gut endoderm hepatoblasts characterized transcriptome lineage. More importantly, primordium as nascent...
Abstract Post-hepatectomy liver dysfunction is a life-threatening morbidity that lacks efficient therapy. Bioactive lipids involved in macrophage polarization crucially regulate tissue injury and regeneration. Herein, we investigate the key bioactive mediate cytotherapeutic potential of polarized-macrophage for post-hepatectomy dysfunction. Untargeted lipidomics identified elevation ceramide (CER) metabolites as signature lipid species relevant to M1/M2 mouse bone-marrow-derived-macrophages...
ABSTRACT The host cytoskeleton plays crucial roles in various stages of virus infection, including viral entry, transport, replication, and release. However, the specific mechanisms by which intermediate filaments are involved orthoflavivirus infection have not been well understood. In this study, we demonstrate that Japanese encephalitis (JEV) remodels vimentin network, resulting formation cage-like structures support replication. Mechanistically, JEV NS1 NS1’ proteins induce translocation...
Abstract Chronic lymphocytic leukaemia (CLL) is a frequent B-cell malignancy, characterized by recurrent somatic chromosome alterations and low level of point mutations. Here we present single-nucleotide polymorphism microarray analyses single CLL patient over 29 years observation treatment, transcriptome whole-genome sequencing at selected time points. We identify 13q14−, 6q− 12q+ in early cell clones, elimination clonal populations following therapy, subsequent appearance clone containing...
Overload of palmitic acids is linked to the dysregulation ceramide metabolism in nonalcoholic steatohepatitis (NASH), and ceramides are important bioactive lipids mediating lipotoxicity acid NASH. However, much remains unclear about role ceramidases that catalyze hydrolysis By analyzing National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database, we found alkaline ceramidase 3 (ACER3) upregulated livers patients with Consistently, Acer3 mRNA levels its...
SUMMARY Host‐induced gene silencing (HIGS) is an inherent mechanism of plant resistance to fungal pathogens, resulting from cross‐kingdom RNA interference (RNAi) mediated by small RNAs (sRNAs) delivered plants into invading fungi. Introducing artificial sRNA precursors crops can trigger HIGS selected genes, and thus has potential applications in agricultural disease control. To investigate the apple ( Malus sp.) during interaction with Botryosphaeria dothidea , pathogenic fungus causing ring...
ABSTRACT Orthoflaviviruses remodel the endoplasmic reticulum (ER) network to construct replication organelles (ROs) for RNA replication. In this study, we demonstrate that Japanese encephalitis virus (JEV) NS1 protein concentrates ER membranes in perinuclear region, which provides a substantial membrane source viral Subsequently, forms main within membrane-concentrated area facilitate efficient This process relies on localization signal, glycosylation, dimerization, and membrane-binding...
Abstract Integrative analysis of multi-omics layers at single cell level is critical for accurate dissection cell-to-cell variation within certain populations. Here we report scCAT-seq, a technique simultaneously assaying chromatin accessibility and the transcriptome same cell. We show that combined signatures enable construction regulatory relationships between cis -regulatory elements target genes single-cell resolution, providing new dimension features helps direct discovery patterns...
ABSTRACT Hypoxia-reoxygenation (H/R) injury in steatotic hepatocytes has been implicated liver dysfunction after transplantation. MicroRNAs (miRs) play important roles regulating several cell biology mechanisms related to H/R injury. However, the role of miRs is still unclear. We established an vitro model for studying and identified miR-34a-5p as a miR that was substantially upregulated under challenge. MiR-34a-5p expression modified by transfecting mimic inhibitor into H/R-challenged...
The application of pulse power discharge (PPD) technology in the crushing and dismantling concrete structures has characteristics related to both green environmental protection, as well safety reliability, with broad prospects construction municipal engineering fields dense urban areas. Nevertheless, research into using this break reinforced (RC) slabs is very limited, while influence key parameters on effect not clear. To solve problem, a finite element model an RC slab was established by...
Traditional agriculture is in the direction of increasing integration primary industry, secondary and tertiary industry Zhejiang province. A survey was undertaken on energy consumption traditional natural villages by taking Anji Ligeng village for an example. This paper firstly studied rural buildings, family structure, occupants' activity usage household appliances form a questionnaire. Then, resource structure were analyzed compared with other surveys. The results show that, electric 6...
Abstract Background: Triple-negative breast cancer (TNBC) represents the most aggressive subtype, which recently attracts great interest for immune therapeutic development. In this context, in-depth understanding of TNBC landscape is highly demanded. Results: Here we report full-length single-cell RNA sequencing results 9683 tumor-infiltrated cells isolated from 14 treatment naïve tumors, where 22 cell subsets, including T cells, macrophages, B and DCs have been characterized. We identify a...
Abstract Background Accurate prediction of epitopes presented by human leukocyte antigen (HLA) is crucial for personalized cancer immunotherapies targeting T cell epitopes. Mass spectrometry (MS) profiling eluted HLA ligands, which provides high-throughput measurements associated peptides in vivo , can be used to faithfully model the presentation on surface. In addition, gene expression profiles measured RNA-seq data a specific cell/tissue type significantly improve performance epitope...
ABSTRACT Triple-negative breast cancer (TNBC) represents the most aggressive subtype, which recently attracts great interest for immune therapeutic development. In this context, in-depth understanding of TNBC landscape is highly demanded. Here we report single-cell RNA sequencing results 9683 tumor-infiltrated cells isolated from 14 treatment naïve tumors, where 22 cell subsets, including T cells, macrophages, B and DCs have been characterized. We identify a new subset, CD8 + CXCL8 cell,...