A5100442271- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA Research and Splicing
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Reproductive Biology and Fertility
- Ionosphere and magnetosphere dynamics
- Single-cell and spatial transcriptomics
- Methane Hydrates and Related Phenomena
- Geophysics and Gravity Measurements
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Cancer-related gene regulation
- Macrophage Migration Inhibitory Factor
- Genomics and Chromatin Dynamics
- Reproductive System and Pregnancy
- Cancer, Hypoxia, and Metabolism
- interferon and immune responses
- Molecular Biology Techniques and Applications
Nanjing Medical University
2014-2025
Chongqing Medical University
2018-2025
The University of Texas Medical Branch at Galveston
2022-2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2018-2025
Jiangsu Province Hospital
2024-2025
Wenzhou Medical University
2014-2025
Zhengzhou Children's Hospital
2025
University of Science and Technology Beijing
2025
Beijing Ditan Hospital
2021-2024
Capital Medical University
2014-2024
Abstract Summary: High-throughput RNA sequencing (RNA-seq) is rapidly emerging as a major quantitative transcriptome profiling platform. Here, we present DEGseq, an R package to identify differentially expressed genes or isoforms for RNA-seq data from different samples. In this package, integrated three existing methods, and introduced two novel methods based on MA-plot detect visualize gene expression difference. Availability: The quick-start vignette available at...
MicroRNAs are involved in various critical functions, including the regulation of cellular differentiation, proliferation, angiogenesis, and apoptosis. We hypothesize that microRNA-210 can rescue cardiac function after myocardial infarction by upregulation angiogenesis inhibition apoptosis heart.Using microRNA microarrays, we first showed was highly expressed live mouse HL-1 cardiomyocytes compared with apoptotic cells 48 hours hypoxia exposure. confirmed polymerase chain reaction robustly...
Malignant glioma belong to the most aggressive neoplasms in humans with no successful treatment available. Patients suffering from glioblastoma multiforme (GBM), highest-grade glioma, have an average survival time of only around one year after diagnosis. Both microglia and peripheral macrophages/monocytes accumulate within but fail exert effective anti-tumor activity even support tumor growth. Here we use microarray analysis compare expression profiles glioma-associated microglia/macrophages...
Do young and old protein molecules have the same probability to be degraded? We addressed this question using metabolic pulse-chase labeling quantitative mass spectrometry obtain degradation profiles for thousands of proteins. find that >10% proteins are degraded non-exponentially. Specifically, less stable in first few hours their life stabilize with age. Degradation conserved similar two cell types. Many non-exponentially (NED) subunits complexes produced super-stoichiometric amounts...
Abstract Inflammatory cytokines, especially tumor necrosis factor α (TNF-α), have been shown to inhibit osteogenic differentiation of mesenchymal stem cells (MSCs) and bone formation in estrogen deficiency–induced osteoporosis, but the mechanism responsible remains poorly understood. MicroRNAs (miRNAs) regulate MSC differentiation. Here, we identified a novel whereby TNF-α, suppressing functional axis key miRNA (miR-21) contributes osteoporosis. In this study, screened differentially...
In response to infections and stress, hematopoiesis rapidly enhances blood immune cell production. The stage within the hematopoietic hierarchy that accounts for this regeneration is unclear under natural conditions in vivo. We analyzed by differentiation tracing, using inducible Tie2- or Flt3-driven Cre recombinase, roles of mouse stem cells (HSCs) multipotent progenitors (MPPs). During polymicrobial sepsis, HSCs responded transcriptionally increased their proliferation death, yet HSC rates...
Abstract Identifying transcriptional enhancers and their target genes is essential for understanding gene regulation the impact of human genetic variation on disease 1–6 . Here we create evaluate a resource >13 million enhancer-gene regulatory interactions across 352 cell types tissues, by integrating predictive models, measurements chromatin state 3D contacts, large-scale perturbations generated ENCODE Consortium 7 We first systematic benchmarking pipeline to compare assembling dataset...
The principal problem arising from prostate cancer (PCa) is its propensity to metastasize bone. MicroRNAs (miRNAs) play a crucial role in many tumor metastases. importance of miRNAs bone metastasis PCa has not been elucidated date. We investigated whether the expression certain was associated with PCa. examined miRNA profiles 6 primary and 7 metastatic samples by microarray analysis. 5 significantly decreased compared PCa, including miRs-508-5p, -145, -143, -33a -100. further other 16 13...
Macroautophagy is an evolutionarily conserved cellular process involved in the clearance of proteins and organelles. Although autophagy regulation machinery has been widely studied, key epigenetic control still remains unknown. Here we report that methyltransferase EZH2 (enhancer zeste 2 polycomb repressive complex subunit) epigenetically represses several negative regulators MTOR (mechanistic target rapamycin [serine/threonine kinase]) pathway, such as TSC2, RHOA, DEPTOR, FKBP11, RGS16 GPI....
Significance How NK cell development diverges from T/B commitment at the common lymphoid progenitor stage is poorly understood. Histone modification near critical gene loci often influences lineage determination. Ezh2 a histone methyltransferase frequently associated with repression. Here we observed that Ezh2-null hematopoietic stem and cells (HSPCs) or HSPCs treated inhibitors gave rise to increased precursors mature progeny display enhanced cytotoxicity against tumor cells. The latter...
Abstract Purpose: Golgi phosphoprotein 3 (GOLPH3) has been reported to be involved in various biologic processes. The clinical significance and role of GOLPH3 breast cancer, however, remains unknown. Experimental Design: Expression normal cells, cancer 6-paired adjacent noncancerous tissues were quantified using real-time PCR Western blotting. protein expression was analyzed 258 archived, paraffin-embedded samples immunohistochemistry. cell proliferation tumorigenicity explored vitro vivo....
Based on the supervisory control theory (SCT) of timed discrete-event systems (TDES), this study presents a dynamic reconfiguration technique for real-time scheduling running uni-processors. A new formalism is developed to assign periodic tasks with multiple-periods. By implementing SCT, system (RTS) dynamically reconfigured when its initial safe execution sequence set empty. During process, based multiple-periods, supervisor proposes different sequences. Two real-world examples illustrate...
Gene expression is precisely controlled in a stage and cell-type-specific manner, largely through the interaction between cis-regulatory elements their associated trans-acting factors. Where these components aggregate promoters enhancers, they are able to cooperate modulate chromatin structure support engagement long-range 3D superstructures that shape dynamics of cell's genomic architecture. Recently, term 'super-enhancer' has been introduced describe hyper-active regulatory domain...