A5063691131- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Immune cells in cancer
- Nerve injury and regeneration
- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Circular RNAs in diseases
- Electrospun Nanofibers in Biomedical Applications
- Bone Metabolism and Diseases
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Hypoxia, and Metabolism
- HIV Research and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Viral Infections and Vectors
- Prion Diseases and Protein Misfolding
- Cannabis and Cannabinoid Research
- Cancer-related Molecular Pathways
- Hematopoietic Stem Cell Transplantation
Florida State University
2016-2025
Florida A&M University - Florida State University College of Engineering
2022-2025
Shaanxi Provincial People's Hospital
2025
Shaanxi Provincial People's Hospital
2025
Shanxi Medical University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Anhui Normal University
2018-2024
Guizhou University
2022-2024
Guizhou Provincial People's Hospital
2012-2024
Shandong First Medical University
2020-2024
Macrophage activation and persistent inflammation contribute to the pathological process of spinal cord injury (SCI). It was reported that M2 macrophages were induced at 3-7 days after SCI but markers reduced or eliminated 1 week. By contrast, M1 macrophage response is rapidly then maintained injured cord. However, factors modulate phenotype function are poorly understood. We developed a model distinguish bone-marrow derived (BMDMs) from residential microglia explored how BMDMs change their...
Alzheimer's disease (AD) is an irreversible neurodegenerative disorder that affects more than 44 million people worldwide. Despite the high burden, there no effective treatment for suffering from AD. Mesenchymal stem cells (MSCs) are multipotent stromal have been widely studied due to their therapeutic potential. However, administration of has found a multitude limitations. Recently, extracellular vesicles (EVs) derived MSCs as candidate, they exhibit similar immunoprotective and...
Human mesenchymal stem cell (hMSC) derived extracellular vesicles (EVs) have shown therapeutic potential in recent studies. However, the corresponding components are largely unknown, and scale-up production of hMSC EVs is a major challenge for translational applications. In current study, hMSCs were grown as 3D aggregates under wave motion to promote EV secretion. Results demonstrate that activation endosomal sorting complexes required transport (ESCRT)-dependent -independent pathways. mRNA...
Hypoxia induced drug resistance is a major obstacle in the development of effective cancer therapy. Our previous study revealed that hypoxia‐inducible factor‐1 (HIF‐1), transcriptional factor significantly activated by hypoxia, was overexpressed gastric vincristine‐resistant cells SGC7901/vincristine (VCR) under normoxic conditions, which suggested it associated with cells. In present study, colony‐forming assay hypoxia and forced HIF‐1α expression increased maximal –8.9‐fold or –14.8‐fold...
MicroRNAs (miRNAs) regulate tumor progression and invasion via direct interaction with target messenger RNAs (mRNAs). We defined miRNAs involved in cancer metastasis (metastamirs) using an established vitro colorectal (CRC) model of minimally metastatic cells (SW480 line) from a colon adenocarcinoma primary lesion highly (SW620 lymph node the same patient 1 year later. used microarray analysis to identify differentially expressed SW480 SW620 cells, focusing on miR-499-5p as novel candidate...
Background: Hepatocellular carcinoma (HCC) is one of the most aggressive cancers, with limited new diagnostic and therapeutic measures.This study aimed to investigate utility specific serum exosome lncRNAs as biomarkers for early diagnosis HCC.Methods: The relative expression levels eight selected in were evaluated by quantitative reverse transcription-PCR (qRT-PCR) training validation sets HCC patients matched healthy controls.Additionally, stability, specificity efficiency these determine...
Aims: To investigate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) transplantation on intrauterine adhesions (IUA). Methods: BMSCs were isolated and labeled by green fluorescence protein. IUA model was established mechanical injury. 48 rats randomly divided into control, model, vein injection groups (n=12 in each group). The third generation injected through tail or intrauterine. Three killed at time 0 h, 7 d, 14 d 28 bilateral uterus obtained points for...
Abstract Methacrylated gelatin (GelMA)/bacterial cellulose (BC) composite hydrogels have been successfully prepared by immersing BC particles in GelMA solution followed photo-crosslinking. The morphology of GelMA/BC hydrogel was examined scanning electron microscopy and compared with pure GelMA. had very well interconnected porous network structure, the pore size decreased from 200 to 10 µm increase content. were also characterized swelling experiment, X-ray diffraction, thermogravimetric...
Abstract Current technologies for acquiring spatial transcript information from tissue sections rely on either RNA probes or barcodes. The former methods require a priori knowledge probeset formulation; the latter have yet to achieve single cell resolution and/or capture efficiencies approaching dissociative, single-cell methods. Here, we describe novel transcriptome assay called p olony (or DNA cluster)- i nde xe d l ibrary-sequencing (PIXEL-seq). It improves upon other barcoding by...
Human mesenchymal stromal cells (hMSCs) are mechanically sensitive undergoing phenotypic alterations when subjected to shear stress, cell aggregation, and substrate changes encountered in 3D dynamic bioreactor cultures. However, little is known about how microenvironment affects the secretion cargo profiles of hMSC-derived extracellular vesicles (EVs) including subset, "exosomes", which contain therapeutic proteins, nucleic acids, lipids from parent cells. In this study, bone marrow-derived...
Abstract Recently, therapies utilizing extracellular vesicles (EVs) derived from mesenchymal stromal/stem cells (MSCs) have begun to show promise in clinical trials. However, EV therapeutic potential varies with MSC tissue source and vitro expansion through passaging. To find the optimal for clinically translatable EV‐derived therapies, this study aims compare angiogenic immunomodulatory potentials protein miRNA cargo compositions of EVs isolated two most common sources adult MSCs, bone...
In this study, we evaluated IL-15 stimulated natural killer cell-derived EVs (NK-EVs) as therapeutic agents in vitro and vivo Osimertinib-resistant lung cancer (H1975R) with EGFR mutations (L858R) combination carboplatin (CBP). NK-EVs were isolated by ultracentrifugation characterized nanoparticle tracking analysis, atomic force microscopy imaging revealed vesicles a spherical form sizes meeting the criteria of exosomal EVs. Further, Western blot studies demonstrated presence regular EV...
Abstract Extracellular vesicles (EVs) secreted by human‐induced pluripotent stem cells (hiPSCs) have great potential as cell‐free therapies in various diseases, including prevention of blood–brain barrier senescence and stroke. However, there are still challenges pre‐clinical clinical use hiPSC‐EVs due to the need for large‐scale production a large quantity. Vertical‐Wheel bioreactors (VWBRs) design features that allow biomanufacturing using scalable aggregate or microcarrier‐based culture...
We investigated whether fetal cells can enter the maternal brain during pregnancy. Female wild-type C57BL/6 mice were crossed with transgenic Green Mice ubiquitously expressing enhanced green fluorescent protein (EGFP). Mouse found in brain. Quantitative real-time polymerase chain reaction (PCR) of genomic DNA for EGFP gene showed that more present 4 weeks postpartum than on day parturition. After an excitotoxic lesion to brain, detected injured region. The presence was also confirmed by...
Neurons and glia in the vertebrate central nervous system arise temporally distinct, albeit overlapping, phases. are generated first followed by astrocytes oligodendrocytes from common progenitor cells. Increasing evidence indicates that axon-derived signals spatiotemporally modulate oligodendrocyte maturation myelin formation. Our previous observations demonstrate F3/contactin is a functional ligand of Notch during maturation, revealing existence another group ligands. Here, we establish...
Hypoxia has been implicated as a crucial microenvironmental factor that induces cancer metastasis. We previously reported hypoxia could promote gastric (GC) metastasis, but the underlying mechanisms are not clear. Long noncoding RNAs (lncRNAs) have recently emerged important regulators of carcinogenesis act on multiple pathways. However, whether lncRNAs involved in hypoxia-induced GC metastasis remains unknown. In this study, we investigated differentially expressed resulting from and...
Abstract Recently, long noncoding RNAs (lncRNAs) are attracting wide attention in the field of cancer research because its important role diagnosis and prognosis. But studies on biological effects relevant mechanisms lncRNAs non‐small cell lung (NSCLC) remain few need to be enriched. Our study discussed expression LncRNA NR2F2‐AS1, further explored possible molecular mechanisms. As a result, elevated NR2F2‐AS1 was detected NSCLC tissues cells remarkably associated with tumor, node,...