A5031723354- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Bone Metabolism and Diseases
- Tissue Engineering and Regenerative Medicine
- Circular RNAs in diseases
- Telomeres, Telomerase, and Senescence
- Periodontal Regeneration and Treatments
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- RNA modifications and cancer
- Bone health and treatments
- Dental Trauma and Treatments
- Epigenetics and DNA Methylation
- Endodontics and Root Canal Treatments
- Wound Healing and Treatments
- Aquaculture Nutrition and Growth
- Muscle Physiology and Disorders
- Cancer-related gene regulation
- Freezing and Crystallization Processes
- Cellular Mechanics and Interactions
- dental development and anomalies
- Food Drying and Modeling
- RNA Research and Splicing
Sichuan University
2003-2025
State Key Laboratory of Oral Diseases
2020-2025
Hainan University
2025
Hubei University of Medicine
2025
Second Affiliated Hospital of Guangzhou Medical University
2024
Guangzhou Medical University
2024
Union Hospital
2024
Hunan Agricultural University
2023-2024
Shanghai University of Traditional Chinese Medicine
2023
Hubei Academy of Agricultural Sciences
2017-2023
Dental pulp regeneration by tooth stem cells promotes recovery of damaged teeth in animal models and human patients.
Mesenchymal stem cell transplantation (MSCT) promotes cutaneous wound healing. Numerous studies have shown that the therapeutic effects of MSCT appear to be mediated by paracrine signaling. However, cell-cell interaction during between MSCs and macrophages in region healing is still unknown. In this study, early depletion delayed repair with MSC injection, which suggested MSC-mediated required macrophages. Moreover, we demonstrated systemically infused bone marrow (BMMSCs) jaw (JMMSCs) could...
Abstract Inflammatory cytokines, especially tumor necrosis factor α (TNF-α), have been shown to inhibit osteogenic differentiation of mesenchymal stem cells (MSCs) and bone formation in estrogen deficiency–induced osteoporosis, but the mechanism responsible remains poorly understood. MicroRNAs (miRNAs) regulate MSC differentiation. Here, we identified a novel whereby TNF-α, suppressing functional axis key miRNA (miR-21) contributes osteoporosis. In this study, screened differentially...
During osteoporosis, the shift of mesenchymal stem cell (MSC) lineage commitment to adipocyte leads imbalance between bone mass and fat, which increases risk fracture. The Enhancer Zeste homology 2 (EZH2), methylates histone H3 on lysine 27 (H3K27me3), controls MSC commitment. However, whether EZH2 is related osteoporosis remains elusive. In our study, we found expression was significantly increased in osteoporotic MSCs. directly H3K27me3 levels promoters Wnt1, Wnt6, Wnt10a silence Wnt gene...
Periodontitis is initiated and sustained by bacteria. However, the mechanism of bacteria induced periodontitis still unknown. We hypothesized that bacterial components can affect functions stem cells in periodontium. In this study, we comparatively investigated influence Lipopolysaccharide (LPS) on osteogenesis potential human periodontal ligament (PDLSCs) bone marrow mesenchymal (BMMSCs).Human PDLSCs BMMSCs were harvested mineralized nodule formation was assessed alizarin red S staining....
During the process of aging, especially for postmenopausal females, cell lineage commitment mesenchymal stem cells (MSCs) shift to adipocyte in bone marrow, resulting osteoporosis. However, cell-intrinsic mechanism this switch is poorly understood. As post-transcription regulation by microRNAs (miRNAs) has a critical role MSCs differentiation and homeostasis, we performed comprehensive miRNAs profiling found miR-705 miR-3077-5p were significantly enhanced from osteoporosis marrow. Both acted...
Systemic infusion of bone marrow-derived mesenchymal stem cells (BMSCs) has become a promising strategy for disease treatment and tissue regeneration. Strategies to enhance the efficiency BMSC cell therapy are crucial promote its clinical application. Here, we aimed improve by inhibiting BMSC-induced coagulation reaction. Intravenous injection gradient BMSCs into mice showed that were not fully compatible with blood. Large doses induced series symptoms respiratory failure heart failure....
Decline of antioxidant defense after estrogen deficiency leads to oxidative damage in bone marrow-derived mesenchymal stem cells (BMMSCs), resulting a defect formation osteoporosis. Forkhead box O1 (FoxO1) protein is crucial for defending physiological bone. But whether FoxO1 involved the during osteoporosis largely unknown. In this study, we found that accumulation was decreased BMMSCs ovariectomized mice. The decrease resulted suppression manganese superoxide dismutase (Sod2) and catalase...
Abstract Disrupted Wnt signaling in osteoblastic-lineage cells leads to bone formation defect osteoporosis. However, the factors repressing are unclear. In our study, we found that was suppressed persistently marrow-derived mesenchymal stem (BMSCs) during Accordingly, histone acetylation levels on genes ( Wnt1 , Wnt6 Wnt10a and Wnt10b ) were declined BMSCs from OVX mice. By screening family of acetyltransferase, identified GCN5 expression increased osteogenic differentiation BMSCs, whereas...
Mesenchymal stem cells (MSCs) are promising candidates for tissue regeneration and disease treatment. However, long-term in vitro passaging leads to stemness loss of MSCs, resulting failure MSCs therapy. Here, we report a melatonin-based strategy improve cell therapy cultured MSCs. Among four small molecules with anti-aging cell-protection properties (rapamycin, resveratrol, quercetin melatonin), colony forming, proliferation, osteogenic differentiation assay showed that melatonin was the...
Abstract MicroRNAs (miRNAs) emerge as important regulators of stem cell lineage commitment and bone development. MiRNA-26a (miR-26a) is one the miRNAs regulating osteogenic differentiation both marrow-derived mesenchymal cells (BMSCs) adipose tissue-derived (ADSCs). However, miR-26a functions oppositely in BMSCs ADSCs, suggesting distinct post-transcriptional regulation tissue-specific MSC differentiation. molecular basis largely unknown. Here, we report that function depended on intrinsic...
Rational: Senescence of mesenchymal stem cells (MSCs) and the related functional decline osteogenesis have emerged as critical pathogenesis osteoporosis in aging.Resveratrol (RESV), a small molecular compound that safely mimics effects dietary restriction, has been well documented to extend lifespan lower organisms improve health aging rodents.However, whether RESV promotes function senescent alleviating age-related phenotypes remains largely unknown.Here, we intend investigate counteracts...
Bone defect caused by trauma, neoplasia, congenital defects, or periodontal disease is a major cause of disability and physical limitation. The transplantation bone marrow mesenchymal stem cells (BMSCs) promotes repair regeneration. However, it has been shown that most BMSCs die within short period after transplantation. During apoptosis, generate large number apoptotic cell–derived extracellular vesicles (ApoEVs). This study aims to understand the potential role ApoEVs in craniofacial...
Abstract Apoptosis releases numerous apoptotic vesicles that regulate processes such as cell proliferation, immunity, and tissue regeneration repair. Now, it has also emerged an attractive candidate for biotherapeutics. However, encompass a diverse range of subtypes, remains unclear which specific subtypes play pivotal role. In this study, we successfully isolated different vesicle based on their sizes characterized them using NTA TEM techniques, respectively. We compared the functional...
Compared with long bone that arises from the mesoderm, major portion of maxillofacial bones and front skull are derived cranial neural crest cells undergo intramembranous ossification. Human skeletal stem have been identified in embryonic fetal bones. Here, we describe a single-cell atlas human mandible identify population cranio-maxillofacial (CMSSCs). These CMSSCs marked by interferon-induced transmembrane protein 5 (IFITM5) specifically located around periosteum jawbone frontal bone....
Osteoporosis, caused by a relative increase of bone resorption over formation, is characterized decreased mass and strength, resulting in an increased incidence fractures, which often leads to further disability early mortality the elderly due impaired healing ability. The majority therapeutics currently used clinics for treatment osteoporosis are antiresorptive agents that exert their clinical effect decreasing rate resorption. However, strategies solely aimed at antiresorption have limited...
Abstract Mutations in the liver/bone/kidney alkaline phosphatase ( Alpl ) gene cause hypophosphatasia (HPP) and early-onset bone dysplasia, suggesting that this is a key factor human development. However, how where acts ageing largely unknown. Here, we determined ablation of induces prototypical premature characteristics, including mass loss marrow fat gain coupled with elevated expression p16 INK4A (p16) p53 due to senescence impaired differentiation mesenchymal stem cells (MSCs)....
Periodontal ligament stem cells (PDLSCs) from periodontitis patients showed defective osteogenic differentiation. However, the mechanism of impaired differentiation PDLSCs in inflammatory microenvironments is still unclear. In this study, we found that inflammation microenvironment resulted downregulation histone acetyltransferase GCN5 expression and lack caused decreased PDLSCs. Previous study activated Wnt/β-cateinin pathway Here knockdown DKK1, an inhibitor pathway, thus Wnt/β-catenin...
Abstract Osteoporosis is caused by pathologic factors such as aging, hormone deficiency or excess, inflammation and systemic diseases like diabetes. Bone marrow stromal cells (BMSCs), the mesenchymal progenitors for both osteoblasts adipocytes, are modulated niche signals. In differential states, pathological characteristics of BMSCs to osteoporoses functional differences unknown. Here, we detected that trabecular bone loss co-existed with increased adiposity in 6 osteoporotic models,...