A5082160785- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA regulation and disease
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Digestive system and related health
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- Metalloenzymes and iron-sulfur proteins
- Nutrition, Genetics, and Disease
- DNA Repair Mechanisms
- Traditional Chinese Medicine Analysis
- Cancer-related gene regulation
- Ginseng Biological Effects and Applications
- Endoplasmic Reticulum Stress and Disease
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Mitochondrial Function and Pathology
- Fish Ecology and Management Studies
- Marine and coastal ecosystems
- Plant Molecular Biology Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
Qilu University of Technology
2025
Shandong Academy of Sciences
2025
First Affiliated Hospital of Guangzhou University of Chinese Medicine
2024
Southern University of Science and Technology
2020-2024
Dalian Medical University
2023
First Affiliated Hospital of Dalian Medical University
2023
Anhui Normal University
2022
The University of Adelaide
2017-2021
Anhui Medical University
2021
Posttranscriptional methylation of RNA cytosine residues to 5-methylcytosine (m5C) is an important modification with diverse roles, such as regulating stress responses, stem cell proliferation, and metabolism. Here, we used bisulfite sequencing for transcriptome-wide quantitative mapping m5C in the model plant Arabidopsis thaliana We discovered more than a thousand sites mRNAs, long noncoding RNAs, other RNAs across three tissue types (siliques, seedling shoots, roots) validated number these...
Astragalus membranaceus, also known as Huangqi in China, is one of the most widely used medicinal herbs Traditional Chinese Medicine. Medicine formulations from membranaceus have been to treat a wide range illnesses, such cardiovascular disease, type 2 diabetes, nephritis and cancers. Pharmacological studies shown that immunomodulating, anti-hyperglycemic, anti-inflammatory, antioxidant antiviral activities exist extract membranaceus. Therefore, characterising biosynthesis bioactive...
Abstract Epithelial–mesenchymal transition (EMT) is a fundamental cellular process frequently hijacked by cancer cells to promote tumor progression, especially metastasis. EMT orchestrated complex molecular network acting at different layers of gene regulation. In addition transcriptional regulation, posttranscriptional mechanisms may also play role in EMT. Here, we performed pooled CRISPR screen analyzing the influence 1,547 RNA-binding proteins on cell motility colon and identified...
Method30 November 2020Open Access Transparent process CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators Liang Fang Corresponding Author [email protected] orcid.org/0000-0003-4502-1756 Department Biology, Southern University Science and Technology, Shenzhen, Guangdong, China Academy Advanced Interdisciplinary Studies, ChinaThese authors contributed equally to this work Search more papers by author Wen Wang Harbin Institute Harbin, Heilongjiang,...
Modified nucleosides in tRNAs are critical for protein translation. N1-methylguanosine-37 and N1-methylinosine-37 tRNAs, both located at the 3'-adjacent to anticodon, formed by Trm5. Here we describe Arabidopsis thaliana AtTRM5 (At3g56120) as a Trm5 ortholog. Attrm5 mutant plants have overall slower growth observed leaf initiation rate, delayed flowering reduced primary root length. In mutants, mRNAs of time genes less abundant correlated with flowering. We show that complements yeast trm5...
Temozolomide (TMZ) resistance is a major obstacle in glioma treatment. Nuclear protein-1 (NUPR1) regulator of progression. This study investigated the mechanism NUPR1 TMZ hypoxia-treated cells and its modulating autophagy. We treated TMZ-resistant U251-TMZ T98G-TMZ to normoxia or hypoxia silenced assess cell viability, proliferation, apoptosis, LC3-II/LC3-I p62 expressions, autophagic flux under different concentrations TMZ. found that upregulated expression autophagy while silencing...
<p>Table S6. The list of differentially expressed proteins upon DDX6 or EDC4 knockout</p>
<p>Table S1. The list of oligos used in the study</p>
<p>Table S4. The list of differentially expressed genes upon DDX6 or EDC4 knockout</p>
<p>Table S1. The list of oligos used in the study</p>
<p>Table S2. The list and sequences of gRNAs</p>
<p>Table S4. The list of differentially expressed genes upon DDX6 or EDC4 knockout</p>
<div>Abstract<p>Epithelial–mesenchymal transition (EMT) is a fundamental cellular process frequently hijacked by cancer cells to promote tumor progression, especially metastasis. EMT orchestrated complex molecular network acting at different layers of gene regulation. In addition transcriptional regulation, posttranscriptional mechanisms may also play role in EMT. Here, we performed pooled CRISPR screen analyzing the influence 1,547 RNA-binding proteins on cell motility colon and...
<p>Table S5. The list of peaks and genes enriched in DDX6-eCLIP-seq</p>
<p>Table S2. The list and sequences of gRNAs</p>
<p>Table S3. The list of candidate genes</p>
<p>Figure S1-S8</p>
<div>Abstract<p>Epithelial–mesenchymal transition (EMT) is a fundamental cellular process frequently hijacked by cancer cells to promote tumor progression, especially metastasis. EMT orchestrated complex molecular network acting at different layers of gene regulation. In addition transcriptional regulation, posttranscriptional mechanisms may also play role in EMT. Here, we performed pooled CRISPR screen analyzing the influence 1,547 RNA-binding proteins on cell motility colon and...
<p>Figure S1-S8</p>
<p>Table S6. The list of differentially expressed proteins upon DDX6 or EDC4 knockout</p>
<p>Table S5. The list of peaks and genes enriched in DDX6-eCLIP-seq</p>
<p>Table S3. The list of candidate genes</p>
The study investigates the therapeutic effects and mechanisms of ginsenoside Rg_1(GRg_1) on sepsis-induced acute lung injury(SALI). A murine model SALI was created using cecal ligation puncture(CLP) surgery, mice were randomly assigned to groups for GRg_1 intervention. Survival body weight changes recorded, function assessed with a non-invasive test system, tissue damage evaluated through HE staining. content expression inflammatory factors measured by ELISA qRT-PCR. Apoptosis examined flow...