A5032564649- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Protein Structure and Dynamics
- Enzyme Structure and Function
- RNA modifications and cancer
- Advanced NMR Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Axon Guidance and Neuronal Signaling
- RNA Research and Splicing
- Computational Drug Discovery Methods
- Chemical Synthesis and Analysis
- Molecular spectroscopy and chirality
- Electron Spin Resonance Studies
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Crystallization and Solubility Studies
- Photoreceptor and optogenetics research
- X-ray Diffraction in Crystallography
- SARS-CoV-2 and COVID-19 Research
- Mass Spectrometry Techniques and Applications
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Photochromic and Fluorescence Chemistry
- NMR spectroscopy and applications
- Prion Diseases and Protein Misfolding
Goethe University Frankfurt
2016-2025
Frankfurt Cancer Institute
2020-2024
Deutschen Konsortium für Translationale Krebsforschung
2013-2024
Instruct-ERIC
2023-2024
Goethe Institute
2007-2024
National Renewable Energy Laboratory
2024
University of Colorado Boulder
2024
Leibniz-Institute for New Materials
2024
Environmental Energy & Engineering
2024
The University of Adelaide
2024
Protein folding and unfolding are coupled to a range of biological phenomena, from the regulation cellular activity onset neurodegenerative diseases. Defining nature conformations sampled in nonnative proteins is crucial for understanding origins such phenomena. We have used combination nuclear magnetic resonance (NMR) spectroscopy site-directed mutagenesis study unfolded states protein lysozyme. Extensive clusters hydrophobic structure exist within wild-type even under strongly denaturing...
Proton-pumping complex I of the mitochondrial respiratory chain is among largest and most complicated membrane protein complexes. The enzyme contributes substantially to oxidative energy conversion in eukaryotic cells. Its malfunctions are implicated many hereditary degenerative disorders. We report x-ray structure at a resolution 3.6 3.9 angstroms, describing detail central subunits that execute bioenergetic function. A continuous axis basic acidic residues running centrally through arm...
The φ,ψ backbone angle distribution of small homopolymeric model peptides is investigated by a joint molecular dynamics (MD) simulation and heteronuclear NMR study. Combining the accuracy measured scalar coupling constants atomistic detail all-atom MD simulations with explicit solvent, thermal populations peptide conformational states are determined an uncertainty <5 %. Trialanine samples mainly (∼90%) poly-l-proline II helix-like structure, some (∼10%) β extended but no αR helical...
Abstract The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights need for coordinated research to combat COVID-19. A particularly important aspect is development of medication. In addition viral proteins, structured RNA elements represent a potent alternative as drug targets. search drugs that target requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, worldwide consortium NMR researchers aims...
Oxidized and reduced hen lysozyme denatured in 8 M urea at low pH have been studied detail by NMR methods. 15N correlated NOESY TOCSY experiments provided near complete sequential assignment for both 1H resonances. Over 900 NOEs, including 130 (i, i + 2) 23 3) could be identified analysis of the spectra states, 3J(HN, Hα) coupling constants relaxation rates measured. The constant NOE data were analyzed comparisons with theoretical predictions from a random coil polypeptide model based on...
Protein phosphatases have very recently emerged as important targets for chemical biology and medicinal chemistry research, new phosphatase inhibitor classes are in high demand. The underlying frameworks of natural products represent the evolutionarily selected fractions space explored by nature so far meet criteria relevance to biological prevalidation most crucial development. We refer synthesis efforts compound collection development based on these biology-oriented synthesis. For...
A protein fusion construct of human ubiquitin with an N-terminal lanthanide binding tag (LBT) enables observation long-range orientational restraints in solution NMR from residual dipolar couplings (RDCs) due to paramagnetic alignment the protein. The ions Tb3+, Dy3+, and Tm3+ are shown bind LBT induce different tensors, agreement theory. RDCs, measured relative diamagnetic Lu3+, range −7.6 5.5 Hz for Tb3+ −6.6 6.1 while opposite tensor is observed (4.5 −2.9 Hz) at 800 MHz. Experimental RDCs...
The WeNMR ( http://www.wenmr.eu ) project is a European Union funded international effort to streamline and automate analysis of Nuclear Magnetic Resonance (NMR) Small Angle X-Ray scattering (SAXS) imaging data for atomic near-atomic resolution molecular structures. Conventional calculation structure requires the use various software packages, considerable user expertise ample computational resources. To facilitate NMR spectroscopy SAXS in life sciences consortium has established standard...
We present a high-resolution nuclear magnetic resonance (NMR) solution structure of 14-mer RNA hairpin capped by cUUCGg tetraloop. This short and very stable presents an important model system for the study dynamics using NMR spectroscopy, molecular (MD) simulations force-field development. The extraordinary high precision (root mean square deviation 0.3 A) could be achieved measuring incorporating all currently accessible parameters, including distances derived from Overhauser effect (NOE)...
A solved puzzle: The structure of the seven-transmembrane-helix proton pump proteorhodopsin obtained by solution NMR spectroscopy is based on NOE data combined with distance restraints derived from paramagnetic relaxation enhancement (see picture). Restraints residual dipolar couplings improved structural accuracy. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available...
The correct target: cell division cycle protein 37 (Cdc37) and the heat shock (Hsp90) are molecular chaperones crucial for folding stabilization of kinases including oncogenic kinases. NMR studies show that celastrol, a recently identified triterpene targeting Hsp90, in fact binds to Cdc37 disrupts Cdc37–Hsp90 complex. Celastrol inactivates through thiol-mediated mechanism. Detailed facts importance specialist readers published as "Supporting Information". Such documents peer-reviewed, but...
Lanthanide-binding tags (LBTs) are peptide sequences of up to 20 encoded amino acids that tightly and selectively complex lanthanide ions can sensitize terbium (Tb3+) luminescence. On the basis these properties, it was predicted increasing number bound lanthanides would improve capabilities tags. Therefore, using a structurally well-characterized single-LBT sequence as starting point, "double-LBT" (dLBT), which concatenates two lanthanide-binding motifs, designed. Herein we report generation...
Lanthanide-binding tags (LBTs) are valuable tools for investigation of protein structure, function, and dynamics by NMR spectroscopy, X-ray crystallography, luminescence studies. We have inserted LBTs into three different loop positions (denoted L, R, S) the model interleukin-1β (IL1β) varied length spacer between LBT 1−3). Luminescence studies demonstrate that all nine constructs bind Tb3+ tightly in low nanomolar range. No significant change fusion occurs from insertion LBT, as shown two...
A reliable intrinsic propensity scale of amino acid residues is indispensable for an assessment how local conformational distributions in the unfolded state can affect folding peptides and proteins. Short host-guest peptides, such as GxG tripeptides, are suitable tools probing propensities. To explore sampled by central residue these motifs, we combined vibrational (IR, Raman, VCD) with NMR spectroscopy. The data were analyzed terms a superposition two-dimensional Gaussian distribution...