A5089278623- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Neuroendocrine Tumor Research Advances
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Liver Disease Diagnosis and Treatment
- Congenital heart defects research
- Epigenetics and DNA Methylation
- Liver physiology and pathology
- TGF-β signaling in diseases
- Digestive system and related health
- Telomeres, Telomerase, and Senescence
- Metabolism, Diabetes, and Cancer
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Genetic factors in colorectal cancer
- Proteoglycans and glycosaminoglycans research
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Fibroblast Growth Factor Research
- Cell death mechanisms and regulation
- Helicobacter pylori-related gastroenterology studies
University of Crete
2011-2024
Orion Corporation (Finland)
2023
University of Turku
2016-2022
Turku Centre for Computer Science
2019-2022
Åbo Akademi University
2019-2022
Turku Centre for Biotechnology
2015-2022
VIB-KU Leuven Center for Cancer Biology
2013-2015
KU Leuven
2008-2015
VIB-KU Leuven Center for Microbiology
2010
National and Kapodistrian University of Athens
2008
Metabolic switches in various immune cell subsets enforce phenotype and function. In the present study, we demonstrate that active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), induces human monocyte-derived tolerogenic dendritic cells (DC) by metabolic reprogramming. Microarray analysis demonstrated 1,25(OH)2D3 upregulated several genes directly related to glucose metabolism, tricarboxylic acid cycle (TCA), oxidative phosphorylation (OXPHOS). Although OXPHOS was promoted...
Abstract Mounting in vitro , vivo and clinical evidence suggest an important role for filopodia driving cancer cell invasion. Using a high-throughput microscopic-based drug screen, we identify FDA-approved calcium channel blockers (CCBs) as potent inhibitors of formation cells. Unexpectedly, discover that L-type channels are functional frequently expressed cells suggesting previously unappreciated these during tumorigenesis. We further demonstrate that, at filopodia, activated by integrin...
Abstract Tissue homeostasis is dependent on the controlled localization of specific cell types and correct composition extracellular stroma. While role cancer stroma in tumour progression has been well characterized, contribution matrix itself unknown. Furthermore, mechanisms enabling normal—not cancer—stroma to provide tumour-suppressive signals act as an antitumorigenic barrier are poorly understood. Here we show that (ECM) generated by normal fibroblasts (NFs) softer than CAF matrix, its...
Tight regulation of integrin activity is paramount for dynamic cellular functions such as cell matrix adhesion and mechanotransduction. Integrin activation achieved through intracellular interactions at the cytoplasmic tails integrin–ligand binding. In this study, we identify metabolic sensor AMP-activated protein kinase (AMPK) a β1-integrin inhibitor in fibroblasts. Loss AMPK promotes activity, formation centrally located active β1-integrin– tensin-rich mature fibrillar adhesions,...
Cell division is a metabolically demanding process, requiring the production of large amounts energy and biomass. Not surprisingly therefore, cell's decision to initiate co-determined by its metabolic status availability nutrients. Emerging evidence reveals that metabolism not only undergoing substantial changes during cell cycle, but it becoming equally clear regulates cycle progression. Here, we overview emerging role those pathways have been best characterized change or influence We then...
Abstract The human epidermal growth factor receptor 2 (HER2) is an oncogene targeted by several kinase inhibitors and therapeutic antibodies. While the endosomal trafficking of many other tyrosine kinases known to regulate their oncogenic signalling, prevailing view on HER2 that this predominantly retained cell surface. Here, we find sortilin-related 1 (SORLA; SORL1 ) co-precipitates with in cancer cells regulates subcellular distribution promoting recycling back plasma membrane. SORLA...
Ductal carcinoma in situ (DCIS) is a pre-invasive stage of breast cancer. During invasion, the encapsulating DCIS basement membrane (BM) compromised, and tumor cells invade surrounding stroma. The mechanisms that regulate functional epithelial BMs vivo are poorly understood. Myosin-X (MYO10) filopodia-inducing protein associated with metastasis poor clinical outcome invasive cancer (IBC). We identify elevated MYO10 expression human IBC, this suggests links disease progression. promotes...
ABSTRACT Fibrillar adhesions are important structural and adhesive components in fibroblasts, required for fibronectin fibrillogenesis. While nascent focal known to respond mechanical cues, the mechanoresponsive nature of fibrillar remains unclear. Here, we used ratiometric analysis paired adhesion determine an appropriate marker. We found that active α5β1-integrin exhibits most definitive localization compared other proteins, such as tensin-1, reported be adhesions. To elucidate...
AimsPlacental growth factor (PlGF), a homologue of vascular endothelial factor, is pleiotropic cytokine with pro-inflammatory activity. Previous gene-inactivation studies revealed that the loss PlGF delays atherosclerotic lesion development and inhibits macrophage infiltration, but activity an anti-PlGF antibody (αPlGF mAb) has not been evaluated yet.
During vessel sprouting, a migratory endothelial tip cell guides the sprout, while proliferating stalk cells elongate branch. Tip and phenotypes are not genetically predetermined fates, but dynamically interchangeable to ensure that fittest (EC) leads sprout. ECs increase glycolysis when forming new blood vessels. Genetic deficiency of glycolytic activator PFKFB3 in reduces vascular sprouting by impairing migration proliferation cells. PFKFB3-driven promotes phenotype during since...
The link between integrin activity regulation and cellular mechanosensing of tissue rigidity, especially on different extracellular matrix ligands, remains poorly understood. Here, we find that primary mouse mammary gland stromal fibroblasts (MSFs) are able to spread efficiently, generate high forces, display nuclear YAP soft collagen-coated substrates, resembling the tissue. We describe loss inhibitor, SHARPIN, impedes MSF spreading specifically type I collagen but not fibronectin. Through...
Abstract Background: Various studies have examined possible correlations between a number of cytokine gene polymorphisms and periodontal disease in populations different origins. The present study sought the correlation four single‐nucleotide ( IL1A +3954, IL1B +4845, TNFA −308, COL1A1 Sp1), variable tandem repeats polymorphism IL1RN intron 2) conditions subjects Greek origin. Methods: One hundred ninety‐two healthy subjects, stratified as non‐periodontitis periodontitis (chronic aggressive)...
Recent evidence indicates that an imbalance between cardiomyocyte hypertrophy and blood vessel growth in the remote myocardium may contribute to heart failure ischaemic disease. It remains, however, largely unknown which angiogenic factors are capable of stimulating after myocardial infarction (MI) whether systemic, rather than local, administration such suffices ameliorate post-MI cardiac recovery. We therefore analysed effect systemic placental factor (PlGF) delivery on recovery mice. MI...
<h3>Background:</h3> Colorectal cancer is one of the most common cancers and third leading cause death in both sexes. The disease progresses as a multistep process associated with genetic alterations. One characteristic features telomerase activation. We sought to evaluate differences activity between colon adjacent normal tissue correlate different locations clinicopathological factors survival. <h3>Methods:</h3> Matched tumour samples mucosa 10 cm away from were collected during colectomy....