A5084258821- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Hippo pathway signaling and YAP/TAZ
- Cancer Cells and Metastasis
- Protein Kinase Regulation and GTPase Signaling
- Chronic Kidney Disease and Diabetes
- Cell Image Analysis Techniques
- Renal Diseases and Glomerulopathies
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Cell death mechanisms and regulation
- Muscle Physiology and Disorders
- Tendon Structure and Treatment
- Pancreatic function and diabetes
- Melanoma and MAPK Pathways
- Advanced Fluorescence Microscopy Techniques
- Proteoglycans and glycosaminoglycans research
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Bone Tissue Engineering Materials
- Microtubule and mitosis dynamics
- Ear and Head Tumors
- Lymphatic System and Diseases
University of Turku
2015-2024
Åbo Akademi University
2017-2024
Turku Centre for Biotechnology
2015-2024
Turku Centre for Computer Science
2019-2024
Kashan University of Medical Sciences
2018
Wellcome Centre for Cell-Matrix Research
2013-2017
University of Manchester
2013-2017
Manchester Academic Health Science Centre
2017
Defective filopodia formation is linked to pathologies such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell dissemination. Despite wide biological significance, delineating function in complex systems remains challenging and particularly hindered by lack of compatible methods quantify properties. Here, we present FiloQuant, a freely available ImageJ plugin, detect filopodia-like protrusions both fixed- live-cell microscopy data. We demonstrate...
Filopodia are adhesive cellular protrusions specialized in the detection of extracellular matrix (ECM)-derived cues. Although ECM engagement at focal adhesions is known to trigger recruitment hundreds proteins ("adhesome") fine-tune behavior, components filopodia remain undefined. Here, we performed a structured-illumination-microscopy-based screen map localization 80 target proteins, linked cell adhesion and migration, within myosin-X-induced filopodia. We demonstrate preferential...
Precise spatiotemporal coordination of integrin adhesion complex dynamics is essential for efficient cell migration. For cells adherent to fibronectin, differential engagement α5β1 and αVβ3 integrins used elicit changes in stability, mechanosensation, matrix assembly, migration, but the mechanisms responsible receptor regulation have remained largely obscure. We identify phosphorylation membrane-intercalated proteoglycan syndecan-4 as an switch controlling recycling. Src phosphorylates and,...
The glomerular basement membrane (GBM) is a specialized extracellular matrix (ECM) compartment within the glomerulus that contains tissue-restricted isoforms of collagen IV and laminin. It integral to capillary wall therefore, functionally linked filtration. Although composition GBM has been investigated with global candidate-based approaches, relative contributions cell types production ECM are not well understood. To characterize specific cellular GBM, we used mass spectrometry–based...
Cellular mechanics play a crucial role in tissue homeostasis and are often misregulated disease. Traction force microscopy is one of the key methods that has enabled researchers to study fundamental aspects mechanobiology; however, traction limited by poor resolution. Here, we propose simplified protocol imaging strategy enhances output increasing i) achievable bead density ii) accuracy tracking. Our approach relies on super-resolution microscopy, fluorescence fluctuation analysis. pipeline...
Cell adhesion to neighbouring cells and the underlying extracellular matrix (ECM) is a fundamental requirement for existence of multicellular organisms. As such, formation, stability dissociation cell adhesions are subject tight control in space time perturbations within sophisticated machinery associated with variety human pathologies. Here, we outline simple protocol monitor alterations ECM, example, following genetic manipulations or overexpression protein interest response drug...
Podocytes are insulin-responsive cells of the glomerular filtration barrier and key in preventing albuminuria, a hallmark feature diabetic nephropathy. While there is evidence that loss insulin signalling to podocytes detrimental, molecular mechanisms underpinning development podocyte resistance diabetes remain unclear. Thus, we aimed further investigate responses early context Conditionally immortalised human mouse cell lines glomeruli isolated from db/db DBA/2J mice were studied. Podocyte...
ABSTRACT Fibrillar adhesions are important structural and adhesive components in fibroblasts, required for fibronectin fibrillogenesis. While nascent focal known to respond mechanical cues, the mechanoresponsive nature of fibrillar remains unclear. Here, we used ratiometric analysis paired adhesion determine an appropriate marker. We found that active α5β1-integrin exhibits most definitive localization compared other proteins, such as tensin-1, reported be adhesions. To elucidate...
Cell-matrix interactions and podocyte intercellular junctions are key for maintaining the glomerular filtration barrier. Vinculin, a cytoplasmic protein, couples actin filaments to integrin-mediated cell-matrix adhesions cadherin-based junctions. Here, we examined role of vinculin in podocytes by generation podocyte-specific knockout mouse. Mice lacking had increased albuminuria foot process effacement following injury vivo. Analysis primary isolated from mutant mice revealed defects cell...
ABSTRACT β1-integrins mediate cell–matrix interactions and their trafficking is important in the dynamic regulation of cell adhesion, migration malignant processes, including cancer invasion. Here, we employ an RNAi screen to characterize regulators integrin traffic identify association Golgi-localized gamma ear-containing Arf-binding protein 2 (GGA2) with β1-integrin, its role recycling active but not inactive β1-integrin receptors. Silencing GGA2 limits levels focal adhesions decreases...
Abstract Increased extracellular matrix (ECM) and stiffness promote solid tumor progression. However, mechanotransduction in cancers arising mechanically active tissues remains underexplored. Here, we report upregulation of multiple ECM components accompanied by tissue stiffening vocal fold cancer (VFC). We compare non-cancerous (NC) patient- derived VFC cells – from early (mobile, T1) to advanced-stage (immobile, T3) revealing an association between progression cell-surface receptor...
Integrin transmembrane receptors control a wide range of biological interactions by triggering the assembly large multiprotein complexes at their cytoplasmic interface. Diverse methods have been used to investigate between integrins and intracellular proteins, predominantly include peptide-based pulldowns biochemical immuno-isolations from detergent-solubilised cell lysates. However, quantitative probe integrin-protein in more biologically relevant context where integrin is embedded within...