A5053767735- Protein Kinase Regulation and GTPase Signaling
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Phagocytosis and Immune Regulation
- Microtubule and mitosis dynamics
- Ion channel regulation and function
- Cancer-related molecular mechanisms research
- Caveolin-1 and cellular processes
- Melanoma and MAPK Pathways
- Erythrocyte Function and Pathophysiology
- RNA modifications and cancer
- Antimicrobial Peptides and Activities
- interferon and immune responses
- Enzyme Structure and Function
- GDF15 and Related Biomarkers
- RNA Research and Splicing
- Click Chemistry and Applications
- Mechanisms of cancer metastasis
- Mycobacterium research and diagnosis
- Cardiac Fibrosis and Remodeling
- Congenital heart defects research
- 3D Printing in Biomedical Research
- Chemical Synthesis and Analysis
Turku Centre for Computer Science
2024-2025
University of Turku
2024-2025
Åbo Akademi University
2024-2025
Turku Centre for Biotechnology
2024
Charles University
2017-2021
Integrin-mediated cell adhesion is essential for migration, mechanotransduction and tissue integrity. In vivo, these processes are regulated by complex physicochemical signals from the extracellular matrix (ECM). These nuanced cues, including molecular composition, rigidity topology, call sophisticated systems to faithfully explore behaviour. Here, we discuss recent methodological advances in cell-ECM research compile a toolbox of techniques that expect shape this field future. We outline...
Cancer's notorious heterogeneity poses significant challenges, as each tumor comprises a unique ecosystem. While single‐cell and spatial transcriptomics advancements have transformed our understanding of diversity within tumors, the temporal dimension remains underexplored. Tumors are dynamic entities that continuously evolve adapt, relying solely on static snapshots obscures intricate interplay between cancer cells their microenvironment. Here, we advocate for integrating dynamics into...
Leukocyte extravasation is a key component of the innate immune response, while circulating tumor cell critical step in metastasis formation. Despite their importance, mechanisms underlying leukocyte and remain incompletely understood. Here, we developed an imaging pipeline that integrates fast, label-free live-cell with deep learning-based image analysis to quantitatively track compare initial steps extravasation—cell landing arrest on endothelial monolayer—under physiological flow...
The tyrosine kinase Src acts as a key regulator of cell motility by phosphorylating multiple protein substrates that control cytoskeletal and adhesion dynamics. In an earlier phosphotyrosine proteomics study, we identified novel Rho-GTPase activating protein, now known ARHGAP42, likely biologically relevant substrate. ARHGAP42 is member family RhoGAPs distinguished tandem BAR-PH domains lying N-terminal to the GAP domain. Like other members, preferentially for RhoA. We show principally...
Protein p130Cas constitutes an adaptor protein mainly involved in integrin signaling downstream of Src kinase. Owing to its modular structure, acts as a general regulator cancer cell growth and invasiveness induced by different oncogenes. However, other mechanisms leading malignant progression are poorly understood. Here, we show novel interaction with Ser/Thr kinase PKN 3, which is implicated prostate breast phosphoinositide 3‐kinase. This direct mediated the SH 3 domain centrally located...
Abstract CAS is a docking protein downstream of the proto-oncogene Src with role in invasion and metastasis cancer cells. The SH3 domain indispensable for CAS-mediated signaling, but structural aspects ligand binding regulation are not well understood. Here, we identified consensus motif structurally characterized complex ligand. We revealed requirement an uncommon centrally localized lysine residue at position +2 ligands two rather dissimilar optional anchoring residues, leucine arginine,...
The ability of cancer cells to adopt various migration modes (the plasticity cell invasiveness) is a substantive obstacle in the treatment metastasis, yet still an incompletely understood process. We performed comparison publicly available transcriptomic datasets from types undergoing switch between mesenchymal and amoeboid modes. Strikingly, lncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) was one three genes that were found upregulated all analyzed. Accordingly,...
Protein kinase N3 (PKN3) is a serine/threonine implicated in tumor progression of multiple cancer types, however, its substrates and effector proteins still remain largely understudied. In the present work we aimed to identify novel PKN3 phosphoproteomic screen using analog sensitive PKN3. Among identified putative selected ARHGAP18, protein from RhoGAP family, for validation further study. We confirmed that can phosphorylate ARHGAP18 vitro also characterized interaction two proteins, which...
ARHGAP42 encodes Rho GTPase activating protein 42 that belongs to a member of the Regulator Associated with Focal Adhesion Kinase (GRAF) family. is involved in blood pressure control by regulating vascular tone. Despite these findings, disorders human variants coding part have not been reported. Here, we describe an 8-year-old girl childhood interstitial lung disease (chILD), systemic hypertension, and immunological findings who carries homozygous stop-gain variant (c.469G>T,...
EPLIN, an actin-binding protein, has been described as both a tumour promoter and suppressor in different cancers. EPLIN isoform(α or β)-specific functions, which remain largely unknown, could explain these opposing roles. We observed distinct EPLIN-isoform localization; EPLINα is recruited to actin plasma membrane ruffles endosomes, while EPLINβ resides on stress fibers. identified two regions demonstrated interaction with Rab21, established regulator of β1-integrin endosomal traffic....
Abstract Protein p130Cas constitutes an adaptor protein mainly involved in integrin signaling downstream of Src kinase. Owing to its modular structure, acts as a general regulator cancer cell growth and invasiveness induced by different oncogenes. However, other mechanisms leading malignant progression are poorly understood. Here, we show novel interaction with Ser/Thr kinase PKN3, which is implicated prostate breast phosphoinositide 3-kinase. This direct mediated the SH3 domain centrally...