A5065713327- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Retinal Development and Disorders
- Protein Kinase Regulation and GTPase Signaling
- Hippo pathway signaling and YAP/TAZ
- Axon Guidance and Neuronal Signaling
- melanin and skin pigmentation
- Photoreceptor and optogenetics research
- Radiopharmaceutical Chemistry and Applications
- Caveolin-1 and cellular processes
- Glycosylation and Glycoproteins Research
- Angiogenesis and VEGF in Cancer
- Biochemical and Structural Characterization
- RNA regulation and disease
- Crystal structures of chemical compounds
- Protease and Inhibitor Mechanisms
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Wnt/β-catenin signaling in development and cancer
- Algal biology and biofuel production
- Ubiquitin and proteasome pathways
- Marine and coastal plant biology
- Biopolymer Synthesis and Applications
Hochschule Bremen
2020-2024
Universität Hamburg
2014-2020
University Medical Center Hamburg-Eppendorf
2014-2020
VTT Technical Research Centre of Finland
2011-2012
University of Turku
2009-2012
Turku Centre for Biotechnology
2011-2012
University of Copenhagen
2012
Leeds Teaching Hospitals NHS Trust
2012
Max Planck Institute of Molecular Physiology
2006-2009
Weatherford College
2009
Integrin trafficking plays an important role in cellular motility and cytokinesis. Integrins undergo constant endo/exocytic shuttling to facilitate the dynamic regulation of cell adhesion. activity toward components extracellular matrix is regulated by ability these receptors switch between active inactive conformations. Several signalling pathways have been described integrin traffic under different conditions. However, interrelationship conformations their endocytic fate remained...
During developmental and tumor angiogenesis, semaphorins regulate blood vessel navigation by signaling through plexin receptors that inhibit the R-Ras subfamily of small GTPases. is mainly expressed in vascular cells, where it induces adhesion to extracellular matrix (ECM) unknown mechanisms. We identify Ras Rab5 interacting protein RIN2 as a key effector endothelial cells interacts with mediates pro-adhesive -angiogenic activity R-Ras. Both R-Ras-GTP localize at nascent ECM sites associated...
Integrin trafficking from and to the plasma membrane controls many aspects of cell behavior including motility, invasion, cytokinesis. Recruitment integrin cargo endocytic machinery is regulated by small GTPase Rab21, but detailed molecular mechanisms underlying recruitment are yet unknown. Here we identify an important role for p120RasGAP (RASA1) in recycling endocytosed α/β1-integrin heterodimers membrane. Silencing attenuated augmented motility. Mechanistically, interacted with...
MINT‐6602303: ARL2 (uniprotkb:P36404) binds (MI:0407) to HRG4 (uniprotkb:Q13432) by pull down (MI:0096) MINT‐6602333: ARL3 (uniprotkb:P36405) CoD (uniprotkb:Q9BTW9) MINT‐6602347, MINT‐6602369: RP2 (uniprotkb:O75695), and physically interact (MI:0218) fluorescence polarization spectroscopy (MI:0053) MINT‐6602195: PDE delta (uniprotkb:O43924) bind MINT‐6602213: BART (uniprotkb:Q8WZ55) MINT‐6602239: MINT‐6602322: MINT‐6602258: (uniprotkb:Q8IWN7) MINT‐6602233: MINT‐6602360: (uniprotkb:Q13432),...
Aneuploidy is frequently detected in solid tumors but the mechanisms regulating generation of aneuploidy and their relevance cancer initiation remain under debate are incompletely characterized. Spatial temporal regulation integrin traffic critical for cell migration cytokinesis. Impaired endocytosis, because loss Rab21 small GTPase or mutations β-subunit cytoplasmic tail, induces failure cytokinesis vitro. Here, we describe that repeatedly failed cytokinesis, impaired traffic, sufficient to...
The γ-aminobutyric acid type B (GABA<sub>B</sub>) receptor is a heterodimeric G-protein-coupled receptor. In humans, three splice variants of the GABA<sub>B</sub> 1 (R1) subunit differ in having one, both, or neither two putative complement control protein (CCP) modules at extracellular N terminus, prior to GABA-binding domain. <i>in vivo</i> function these predicted remains be discovered, but likely association with matrix proteins intriguing. portion R1a variant encompassing both its CCP...
Abstract Synaptic plasticity and memory formation involve remodeling of the postsynaptic cytoskeleton, a process that is in part based on both local translation dendritic mRNAs synaptic recruitment newly synthesized proteins. The component Dendrin encoded by dendritically localized mRNA thought to modulate structure cytoskeleton. However, molecular mechanisms control extrasomatic transport protein are unknown. data presented here reveal interacts with cytoskeletal components α‐actinin Maguk...
GTPases are central cellular signaling proteins, which cycle between a GDP-bound inactive and GTP-bound active conformation in controlled manner. Ras frequently mutated cancer so far only few experimental inhibitors exist. The most common methods for monitoring GTP hydrolysis rely on luminescent GDP- or GTP-analogs. In this study, the first GTP-specific Fab fragment its application described. We selected fragments using phage display technology. Six were found against 2'/3'-GTP-biotin...
Abstract Despite advancements in RNA extraction methods, from sources rich polyphenols and polysaccharides such as algae seagrasses remains a challenge. Here we present strategy using hexadecyltrimethylammonium bromide (CTAB) buffer demonstrate its effectiveness on broad range of red, green, brown algae, well the cyanobacterium Arthrospira platensis seagrass Zostera marina . For vast majority tested samples achieved high yields comparable to those obtained higher plants by commercially...
Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis, and cancer invasion often hijacked by viral infections 1 . Unlike clathrin-mediated endocytosis, which exploits adaptors selective protein internalization, the clathrin dynamin-independent endocytic pathway (CLIC-GEEC, CG-pathway) has until now been considered a bulk internalization route fluid phase, glycosylated membrane proteins lipids 2,3 Although core molecular players of CG have recently...
EPLIN, an actin-binding protein, has been described as both a tumour promoter and suppressor in different cancers. EPLIN isoform(α or β)-specific functions, which remain largely unknown, could explain these opposing roles. We observed distinct EPLIN-isoform localization; EPLINα is recruited to actin plasma membrane ruffles endosomes, while EPLINβ resides on stress fibers. identified two regions demonstrated interaction with Rab21, established regulator of β1-integrin endosomal traffic....
During developmental and tumor angiogenesis, semaphorins regulate blood vessel navigation by signaling through plexin receptors that inhibit the R-Ras subfamily of small GTPases. is mainly expressed in vascular cells, where it induces adhesion to extracellular matrix (ECM) unknown mechanisms. We identify Ras Rab5 interacting protein RIN2 as a key effector endothelial cells interacts with mediates pro-adhesive -angiogenic activity R-Ras. Both R-Ras-GTP localize at nascent ECM sites associated...