A5089862690- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Vector-borne infectious diseases
- Caveolin-1 and cellular processes
- Erythrocyte Function and Pathophysiology
- Protein Kinase Regulation and GTPase Signaling
- Ubiquitin and proteasome pathways
- Viral Infections and Vectors
- Toxin Mechanisms and Immunotoxins
- Cardiomyopathy and Myosin Studies
- Microfluidic and Bio-sensing Technologies
- Wnt/β-catenin signaling in development and cancer
- Galectins and Cancer Biology
- Phagocytosis and Immune Regulation
- Vector-Borne Animal Diseases
- Antimicrobial Resistance in Staphylococcus
- Cancer, Hypoxia, and Metabolism
- Skin and Cellular Biology Research
- Angiogenesis and VEGF in Cancer
- Advanced DC-DC Converters
Universität Hamburg
2016-2025
University Medical Center Hamburg-Eppendorf
2016-2025
Roche Pharma AG (Germany)
2025
Eppendorf (Germany)
2013-2023
Institut für Medizinische Informatik, Biometrie und Epidemiologie
2022
Kiel University
2022
Universitäre Psychiatrische Dienste Bern
2018
Institute of Medical Microbiology and Hygiene
2001-2014
University Medical Center
2010-2013
University of Cologne
2013
Wiskott-Aldrich syndrome protein (WASp) is a hematopoietic-specific, multidomain whose mutation responsible for the immunodeficiency disorder syndrome. WASp contains binding motif Rho GTPase CDC42Hs as well verprolin/cofilin-like actin-regulatory domains, but no specific actin structure regulated by CDC42Hs-WASp has been identified. We found that colocalizes with and in core of podosomes, highly dynamic adhesion human blood-derived macrophages. Microinjection constitutively active V12CDC42Hs...
Salmonella typhimurium translocates effector proteins into host cells via the SPI1 type III secretion system to induce responses such as membrane ruffling and internalization by non‐phagocytic cells. Activation of cellular RhoGTPase Cdc42 is thought be a key event during internalization. The translocated protein SopE an activator for Cdc42. Because absent from most S. strains it remains unclear whether all rely on activation invade We have identified SopE2, common strains. SopE2 guanine...
This article is part of a Minifocus on invadopodia and podosomes. For further reading, please see related articles: `Matrix invasion by tumour cells: focus MT1-MMP trafficking to invadopodia' Renaud Poincloux et al. (J. Cell Sci. 122, 3015-3024), `Mechanisms for transcellular diapedesis: probing pathfinding `invadosome-like protrusions” Christopher V. Carman 3025-3035) `Actin machinery mechanosensitivity in invadopodia, podosomes focal adhesions' Corinne Albiges-Rizo 3037-3049).Podosomes...
ABSTRACT Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteinases that belong to the group endopeptidases or matrixins. They able cleave plethora substrates, including components extracellular matrix and cell-surface-associated proteins, as well intracellular targets. Accordingly, MMPs play key roles in variety physiological pathological processes, such tissue homeostasis cancer cell invasion. MMP activity is exquisitely regulated at several levels, pro-domain removal,...
In the dynamic field of gene therapy, recombinant adeno-associated viruses (rAAVs) have become leading viral vectors due to their safety, long-term expression, and wide-ranging cell tissue tropism. With numerous FDA approvals commercial products underscoring potential, there is a critical need for efficient production processes achieve high vector titers quality. A major challenge in rAAV packaging genome into capsid, with empty or partially filled capsids often representing over 90%...
ABSTRACT Podosomes are unique actin-rich adhesion structures of monocyte-derived cells such as macrophages and osteoclasts. They clearly differ from other substratum-contacting organelles like focal adhesions in morphological functional regards. Formation podosomes has been shown to be dependent on the small GTPase CDC42Hs its effector Wiskott-Aldrich syndrome protein (WASp). In this study, we investigated relation between microtubule system primary human macrophages. We demonstrate that,...
Abstract Wiskott-Aldrich syndrome (WAS) is an X-linked recessive disorder originally characterized by the clinical triad eczema, thrombocytopenia, and severe immunodeficieny, with recurrent bacterial viral infections, indicating a profound immune cell defect. Such altered cells include monocytes, macrophages, dendritic cells, which were reported to display disturbed polarization or chemotaxis. WAS caused mutations in protein (WASp), thought organize actin cytoskeleton through Arp2/3 complex....
Microtubules are important for the turnover of podosomes, dynamic, actin-rich adhesions implicated in migration and invasion monocytic cells. The molecular basis this functional dependency, however, remained unclear. Here, we show that contact by microtubule plus ends critically influences cellular fate podosomes primary human macrophages. In particular, identify kinesin KIF1C, a member Kinesin-3 family, as plus-end–enriched motor targets regions podosome turnover. Expression mutation...
Podosomes are actin-rich adhesion and invasion structures. Especially in macrophages, podosomes exist two subpopulations, large precursors at the cell periphery smaller (successors) interior. To date, mechanisms that differentially regulate these subpopulations largely unknown. Here, we show membrane-associated protein supervillin localizes preferentially to successor becomes enriched immediately prior their dissolution. Consistently, podosome numbers inversely correlated with levels. Using...