A5015835780- Glycosylation and Glycoproteins Research
- Ovarian cancer diagnosis and treatment
- Galectins and Cancer Biology
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Cervical Cancer and HPV Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Proteoglycans and glycosaminoglycans research
- Cancer-related molecular mechanisms research
- HER2/EGFR in Cancer Research
- Caveolin-1 and cellular processes
- Cancer Genomics and Diagnostics
- Brain Metastases and Treatment
- Circular RNAs in diseases
- Cellular transport and secretion
- Lung Cancer Research Studies
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Glioma Diagnosis and Treatment
- Extracellular vesicles in disease
- Lymphatic System and Diseases
- Carbohydrate Chemistry and Synthesis
Universität Hamburg
2016-2025
University Medical Center Hamburg-Eppendorf
2016-2025
Klinik und Poliklinik für Neurologie
2016-2020
Eppendorf (Belgium)
2015
University Medical Center
2015
University Cancer Center Hamburg
2011-2014
Essen University Hospital
2007
Neurology, Inc
2004
Specific micro RNA s (mi s) are packaged in exosomes that regulate processes tumor development and progression. The current study focuses on the influence of exosomal mi pathogenesis epithelial ovarian cancer ( EOC ). Mi profiles were determined from plasma 106 patients, eight cystadenoma 29 healthy women by TaqMan real‐time PCR ‐based array cards containing 48 different s. In cell culture experiments, impact miR‐200b miR‐320 was proliferation apoptosis lines. We report miR‐21 P = 0.0001),...
Long noncoding RNAs (lncRNAs) are frequently precursor of microRNAs (miRNAs) or act as competing endogenous (ceRNAs) to interact with miRNAs. To better understand the shared impact lncRNAs and miRNAs in regulatory post‐transcriptional network, we focused here on relationships between (a) lncRNA H19 miR‐675, (b) NEAT1 miR‐204, (c) HOTAIR miR‐331 plasma early breast cancer (BC) patients. We quantified each RNA samples 63 BC patients 10 healthy women by quantitative real‐time PCR. In cell...
Immune checkpoints are intensively investigated as targets in cancer therapy. T-cell immunoreceptor with immunoglobulin (Ig) and ITIM domains (TIGIT) its ligand poliovirus receptor (PVR) recently emerging novel promising immunotherapy. Here, we show that high expression of PVR represents an independent prognostic marker being associated poor outcome for breast patients. Furthermore, mRNA, well protein expression, is more aggressive subtypes such HER2 positive triple-negative cancer. In...
Abstract Background The antiangiogenic agent cilengitide disrupts integrin binding to the extracellular matrix leading apoptosis of activated endothelial cells. Integrins are also widely expressed in malignant glioma and inhibitors may directly target tumor cells this disease. Aim current study was investigate effects on molecular cellular levels. Results Cilengitide caused dose-dependent detachment from cell culture dishes. Proliferation significantly inhibited while proportion apoptotic...
C-Fos was initially described as oncogene, but associated with favourable prognosis in ovarian cancer (OvCa) patients. The molecular and functional aspects underlying this effect are still unknown. Using stable transfectants of SKOV3 OVCAR8 cells, proliferation, migration, invasion apoptotic potential c-FOS-overexpressing clones controls were compared. Adherence to components the extracellular matrix analysed static assays, adhesion E-selectin, endothelial mesothelial cells dynamic flow...
Loading of microRNAs (miRNAs) into exosomes that are involved in cellular communication is a selective process. The current study investigates whether the enrichment miRNAs reflects pathogenesis breast cancer (BC) and ductal carcinoma situ (DCIS). levels were quantified from plasma 32 BC patients, 8 DCIS patients healthy women by TaqMan real-time PCR-based miRNA array cards containing 47 different miRNAs. Then, exosomal miR-16, miR-30b miR-93 displayed deregulation arrays selected analyzed...
Abstract Background Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules tumor metastasis. The aim our study was to investigate role activated leukocyte molecule (ALCAM) BM formation NSCLC. Methods Immunohistochemical analysis performed on 143 NSCLC primary tumors and BM. A correlation between clinicopathological parameters survival developed. Biological properties ALCAM were assessed...
Phenotypic characterization of γδ T cells in the MALs (malignant ascites lymphocytes), TILs (tumor infiltrating and PBLs (peripheral blood lymphocytes) ovarian cancer (OvCA) patients is lacking. Therefore, we quantified cell prevalence MAL, TIL, PBL specimens from n = 18 OvCA age-matched healthy donors (HD, 14). Multicolor flow cytometry was performed to evaluate expression inhibitory receptors (TIGIT, PD-1 TIM-3), stimulatory (Ox40), purinergic ectoenzymes (CD39 CD73) on subsets. We...
Metastasis is the leading cause of cancer-related death in non-small cell lung cancer (NSCLC) patients. We previously showed that low HERC5 expression predicts early tumor dissemination and a dismal prognosis NSCLC Here, we performed functional studies to unravel mechanism underlying "metastasis-suppressor" effect HERC5, with focus on mitochondrial metabolism pathways. assessed proliferation, colony formation potential, anchorage-independent growth, migration, wound healing line models...
Abstract Here, we show that carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is expressed in umbrella cells of bladder urothelium but down-regulated superficial cancer, such as histologic tumor stage a (pTa) and transitional carcinoma situ (pTis). Concurrently, CEACAM1 up-regulated the endothelia adjacent angiogenic blood vessels. Mimicking down-regulation urothelium, was silenced cancer lines 486p RT4 using small interfering RNA technique. confirmed at protein level by...
Identification and characterization of biomarkers in body fluids such as serum or urine serve a basis for early detection diseases, particularly cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting immunohistochemistry we identified two proteins, orosomucoid (ORM) human zinc-alpha(2)-glycoprotein (ZAG), which were increased the samples patients bladder cancer comparison to healthy volunteers. The highest amount both proteins was found invasive stages pT2-3....
Alterations in protein glycosylation have been related to malignant transformation and tumour progression. We recently showed that low mRNA levels of Golgi alpha-mannosidase MAN1A1 correlate with poor prognosis breast cancer patients.We analysed the role on a level using western blot analysis (n=105) studied impact MAN1A1-related prognostic relevance adhesion molecules involved microarray data (n=194). Functional consequences mannosidase inhibition inhibitor kifunensine or silencing were...
Aberrant sialylation of glycoproteins has been detected in many tumors, and upregulation the beta-galactosamide alpha-2,6-sialyltransferase 1 (ST6GAL1) implicated with tumor aggressiveness chemoresistance experimental models. In our present study, we aimed to study prognostic or predictive role ST6GAL1 ovarian carcinoma, using two independent cancer cohorts. mRNA levels were retrieved from a publicly available database (n = 517), protein analyzed by western blot analysis cohort 204 samples....
The standard of care for ovarian cancer comprises cytoreductive surgery, followed by adjuvant platinum-based chemotherapy plus taxane therapy and maintenance with the antiangiogenic compound bevacizumab and/or a PARP inhibitor. Nevertheless, there is currently no clear clinical indication use bevacizumab, highlighting urgent need biomarkers to assess response bevacizumab. In present study, based on novel RNA-seq dataset (n=181) previously published microarray-based (n=377), we have...
Abstract Objective In advanced ovarian cancer, the majority of patients receive anti-angiogenic treatment with bevacizumab. However, its use is often associated severe side effects, and not all benefit from therapy. Currently, there are no reliable biomarkers to predict response treatment. Given their role as key regulators angiogenesis, platelet-derived growth factor receptor-beta (PDGFR-beta) vascular endothelial receptor-2 (VEGFR-2) promising candidates for predictive biomarkers. This...
Abstract L-arginine limits proliferation in highly proliferative tissues. It is a substrate for nitric oxide synthases, arginases; its methylation by protein-L-arginine methyltransferases (PRMTs) leads to asymmetric (ADMA) and symmetric dimethylarginine (SDMA). We measured metabolites L-ornithine, L-citrulline, ADMA, SDMA prospective cohort of 243 women with primary breast cancer (BC) their associations mortality disease recurrence during 88 (IQR, 82–93) months follow-up. quantified these...
Peritoneal dissemination and retroperitoneal lymph node involvement are main routes for tumour spread of epithelial ovarian cancer (EOC), possibly determined by the intercellular connecting protein E-Cadherin (E-Cad) its fragments. Tumour tissue 105 advanced EOC patients was evaluated expression E-Cad, β-Catenin Calpain western blotting immunohistochemistry. Expression patterns were compared between tumours with solely intraperitoneal (pT3c, pN0; n=41) metastases (pT1a-3c, pN1; n=64)....
Abstract Background The survival rate is poor in breast cancer patients with brain metastases. Thus, new concepts for therapeutic approaches are required. During metastasis, the cytoskeleton of cells highly dynamic and therefore cytoskeleton-associated proteins interesting targets tumour therapy. Methods Screening genes showing a significant correlation metastasis formation was performed based on microarray data from long-term follow up information. Validation most target by MTT-, Scratch-...