A5065562049- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Bladder and Urothelial Cancer Treatments
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Prostate Cancer Treatment and Research
- Advanced biosensing and bioanalysis techniques
- Ovarian cancer diagnosis and treatment
- Cancer Research and Treatments
- Periodontal Regeneration and Treatments
- DNA Repair Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Renal and related cancers
- Prostate Cancer Diagnosis and Treatment
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
University Medical Center Hamburg-Eppendorf
2015-2024
Universität Hamburg
2015-2024
University Cancer Center Hamburg
2013-2015
Heinrich Heine University Düsseldorf
2012
Düsseldorf University Hospital
2012
Ludwig-Maximilians-Universität München
2012
Praxis
2011
In-Q-Tel
2009-2010
University Medical Center
2008
Eppendorf (Germany)
2006
Abstract Introduction MicroRNAs (miRs) are interesting new diagnostic targets that may provide important insights into the molecular pathogenesis of breast cancer. Here we evaluated, for first time, feasibility and clinical utility circulating miRs as biomarkers detection staging Methods The relative concentrations cancer-associated miR10b, miR34a, miR141 miR155 were measured in blood serum 89 patients with primary cancer (M0, n = 59) metastatic disease (M1, 30), 29 healthy women by a TaqMan...
// Corinna Eichelser 1, * , Isabel Stückrath Volkmar Müller 2 Karin Milde-Langosch Harriet Wikman 1 Klaus Pantel Heidi Schwarzenbach Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Germany Clinic Gynecology, Hamburg, These authors contributed equally to this work Correspondence to: Schwarzenbach, e-mail: hschwarz@uke.uni-hamburg.de Keywords: cell-free miRs, exosomal estrogen receptor, luminal breast cancer Received: June 27,...
Exosomes are membrane vesicles that mediate intercellular communication by transporting their molecular cargo from cell to cell. We investigated whether serum levels of exosomal miR-373, miR-200a, miR-200b and miR-200c circulating exosomes have diagnostic prognostic relevance in a cohort 163 epithelial ovarian cancer (EOC) patients using TaqMan MicroRNA assays ELISA. The concentrations miR-373 (p = 0.0001), miR-200a 0.0001) 0.028) were significantly higher EOC than healthy women. 0.019)...
The focus of this study is to identify particular microRNA (miRNA) signatures in exosomes derived from plasma 435 human epidermal growth factor receptor 2 (HER2)-positive and triple-negative (TN) subtypes breast cancer (BC).First, miRNA expression profiles were determined the 15 TNBC patients before neoadjuvant therapy using a quantitative TaqMan real-time PCR-based array card containing 384 different miRNAs. Forty-five miRNAs associated with clinical parameters then selected mounted on...
Abstract Purpose: Circulating cell-free DNA in the blood of cancer patients harbors tumor-specific aberrations. Here, we investigated whether this might also reflect presence circulating tumor cells (CTC). Experimental Design: To identify source blood, plasma derived from 81 with prostate was examined for CTCs and DNA. An epithelial immunospot assay applied detection CTCs, a PCR-based fluorescence microsatellite analysis panel 14 polymorphic markers used allelic imbalances (AI). Results: The...
MicroRNAs (miRs) are small, noncoding RNAs that target genes involved in tumor development and progression. In the current study, we investigated use of circulating miR concentrations as biomarkers serum breast cancer patients.We analyzed samples from 120 patients with primary after surgery before chemotherapy (M0, classified into 3 subgroups 40 progesterone/estrogen-positive, HER2-positive, triple-negative cancer), 32 overt metastasis (M1), healthy women. Using quantitative TaqMan MicroRNA...
The broad range of expression cancer-testis antigens in various tumor types makes the proteins encoded by human MAGE gene family promising targets for anticancer immunotherapy. However, a major drawback is their heterogeneous expression. In current study, we have examined influence DNA methylase inhibitor 5-aza-2'-deoxycytidine (5-aza-CdR) together with histone deacetylase trichostatin A on MAGE-A1, -A2, -A3, and -A12 genes different cell lines. Reverse transcription-PCR, Western blot...
Cell‐free tumor‐specific and normal DNA are released into the blood circulation by cellular necrosis apoptosis. We examined whether circulating in of patients with colorectal cancer could be used as an additional marker for diagnosis response to therapy. The concentration cell‐free 55 advanced 20 healthy donors was measured. One four follow‐up serum samples from 14 were also available. Patients had a wide range concentrations their blood. calculated values between 22 3922 ng/mL DNA, mean...
Specific micro RNA s (mi s) are packaged in exosomes that regulate processes tumor development and progression. The current study focuses on the influence of exosomal mi pathogenesis epithelial ovarian cancer ( EOC ). Mi profiles were determined from plasma 106 patients, eight cystadenoma 29 healthy women by TaqMan real‐time PCR ‐based array cards containing 48 different s. In cell culture experiments, impact miR‐200b miR‐320 was proliferation apoptosis lines. We report miR‐21 P = 0.0001),...
Owing to late diagnosis in advanced disease stages, prognosis of patients with epithelial ovarian cancer (EOC) is poor. The quantification deregulated levels microRNAs could facilitate earlier and improve EOC. Seven (miR-7, miR-16, miR-25, miR-93, miR-182, miR-376a miR-429) were quantified the serum 180 EOC 66 healthy women by TaqMan PCR microRNA assays. Median follow-up time was 21 months. effects miR-7 miR-429 on apoptosis, cell proliferation, migration invasion investigated two lines....
Long noncoding RNAs (lncRNAs) are frequently precursor of microRNAs (miRNAs) or act as competing endogenous (ceRNAs) to interact with miRNAs. To better understand the shared impact lncRNAs and miRNAs in regulatory post‐transcriptional network, we focused here on relationships between (a) lncRNA H19 miR‐675, (b) NEAT1 miR‐204, (c) HOTAIR miR‐331 plasma early breast cancer (BC) patients. We quantified each RNA samples 63 BC patients 10 healthy women by quantitative real‐time PCR. In cell...
// Isabel Stückrath 1 , Brigitte Rack 2 Wolfgang Janni 3 Bernadette Jäger Klaus Pantel and Heidi Schwarzenbach Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Germany First Obstetrics Gynecology, Ludwig Maximilians Munich, Gynecology Obstetrics, Hospital Ulm, Correspondence to: Schwarzenbach, email: Keywords : cell-free miRs, chemotherapy, invasion, proliferation, tumor progression Received December 22, 2014 Accepted April 03, 2015 Published 19, Abstract Within the...
Treatment of mesangial cells with recombinant human interleukin 1β (IL‐1β) or tumor necrosis factor α (TNFα) dose‐dependently increased cGMP formation. Both IL‐1β and TNFα‐stimulated formation occurred after a initial lag period 4 to 8 hours. actinomycin D, cycloheximide dexamethason completely abolished cytokine‐induced Furthermore, the guanylate cyclase inhibitor Methylene blue blocked IL‐1β‐ generation. N G ‐mono‐methyl‐L‐arginine attenuated TNFα‐induced production, an effect that was...