A5020728152- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- Toxin Mechanisms and Immunotoxins
- Epigenetics and DNA Methylation
- Transgenic Plants and Applications
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Circular RNAs in diseases
- Galectins and Cancer Biology
- Prostate Cancer Treatment and Research
- Angiogenesis and VEGF in Cancer
- Proteoglycans and glycosaminoglycans research
- Exercise and Physiological Responses
- Plant Virus Research Studies
- 3D Printing in Biomedical Research
- Cancer Research and Treatments
- DNA and Nucleic Acid Chemistry
- Phytochemical Studies and Bioactivities
- Wnt/β-catenin signaling in development and cancer
National Research University Higher School of Economics
2020-2025
Institute of Bioorganic Chemistry
2019-2023
P.A. Hertzen Moscow Oncology Research Institute
2023
BioClinicum (Russia)
2012-2022
Medical Radiological Research Center
2018-2019
Far Eastern Federal University
2018
Federal Scientific Center of Physical Culture and Sports
2011
Lomonosov Moscow State University
2006-2010
Constructor University
2009
Stony Brook University
2006
Abstract Background MiRNAs are essential mediators of many biological processes. The aim this study was to investigate the dynamics miRNA-mRNA regulatory networks during exercise and subsequent recovery period. Results Here we monitored transcriptome changes using microarray analysis whole blood eight highly trained athletes before after 30 min moderate followed by 60 We combined expression profiling bioinformatics analysed metabolic pathways enriched with differentially expressed mRNAs...
C-Fos was initially described as oncogene, but associated with favourable prognosis in ovarian cancer (OvCa) patients. The molecular and functional aspects underlying this effect are still unknown. Using stable transfectants of SKOV3 OVCAR8 cells, proliferation, migration, invasion apoptotic potential c-FOS-overexpressing clones controls were compared. Adherence to components the extracellular matrix analysed static assays, adhesion E-selectin, endothelial mesothelial cells dynamic flow...
Significance Post-traumatic sterile inflammation is the first necessary step of wound healing. In addition, underlies pathogenesis a multitude common diseases, such as allergies and autoimmune diseases. The molecular mechanisms underlying are still not fully understood. Here, we show that receptor Mincle (Clec4e), expression which highly induced in skin response to damage, recognizes cholesterol sulfate, molecule abundant epidermal layer skin, subsequently inducing pro-inflammatory response....
Quantification and normalization of RT-qPCR data critically depends on the expression so called reference genes. Our goal was to develop a strategy for selection genes that utilizes microarray analysis combines known approaches gene stability evaluation select set appropriate research clinical breast samples with different receptor cancer status using this strategy. A preliminary search based high-throughput datasets. The final validation candidate were several methods evaluation:...
Detailed characterization of cell type transitions is essential for biology in general and particularly the development stem cell-based therapies regenerative medicine. To systematically study such transitions, we introduce a method that simultaneously measures protein expression thermal stability changes cells provide web-based visualization tool ProteoTracker. We apply our to differences between human pluripotent several types including their parental line differentiated progeny. detect...
7,8-Dihydro-8-oxoguanine (8-oxoG) is a ubiquitous oxidative DNA lesion resulting from injury to via reactive oxygen species. 8-oxoG lesions may play role in the formation of aberrant methylation patterns during carcinogenesis. In this study, we assessed effects on and complex nine 30-mer oligodeoxynucleotide duplexes by catalytic domain murine Dnmt3a methyltransferase (Dnmt3a-CD). The rate hemimethylated varied 25-fold decrease 1.8-fold increase, depending position relative Dnmt3a-CD...
Laminins are a major family of extracellular matrix proteins and the main component basement membranes. involved in many if not all stages cancer progression, expression laminin genes has prognostic value various types cancer, including colorectal. Only single or components trimer with significant differential have been regarded as potential biomarkers to date. Here we compared power classifiers constructed from sets that any gene. The analysis showed cumulative was higher achieved already...
Inflammatory breast cancer (IBC) is an extremely malignant form of which can be easily misdiagnosed. Conclusive prognostic IBC molecular biomarkers are also providing the perspectives for targeted therapy lacking so far. The aim this study was to reveal IBC-specific miRNA expression profile and evaluate its association with clinicopathological parameters. profiles 13 17 non-IBC patients were characterized using comprehensive Affymetrix GeneChip 3.0 microarray platform. Bioinformatic analysis...
A cancer cell line originating from human epithelial colorectal adenocarcinoma (Caco-2 cells) serves as a high capacity model for preclinical screening of drugs. Recent need incorporating barrier tissue into multi-organ chips calls inclusion Caco-2 cells microperfused environment. This article describes series systems biology insights obtained comparing models grown conventional 2D layer and in microfluidic chip. When basic electrical parameters monolayers were evaluated using impedance...
Intestinal epithelial cells exist in physiological hypoxia, leading to hypoxia-inducible factor (HIF) activation and supporting barrier function cell metabolism of the intestinal epithelium. In contrast, pathological hypoxia is a common feature some chronic disorders, including inflammatory bowel disease (IBD). This work was aimed at studying HIF-associated changes epithelium IBD. first step, list genes responding chemical obtained an vitro model with RNA sequencing. Cobalt (II) chloride...
Astrocytes are considered to be an important contributor central nervous system (CNS) disorders, particularly multiple sclerosis. The transcriptome of these cells is greatly affected by cytokines released lymphocytes, penetrating the blood-brain barrier-in particular, classical pro-inflammatory cytokine interferon-gamma (IFNγ). We report here transcriptomal profiling astrocytes treated using IFNγ and benztropine, a putative remyelinization agent. Our findings indicate that expression genes...
When located in the DNA minor groove, dimeric bisbenzimidazoles DB(n) effectively inhibited vitro Dnmt3a catalytic domain (IC50 5–77 μM). The lowest IC50 value was observed for compound DB(11) with an 11-unit methylene linker joining bisbenzimidazole fragments. Increased time of incubation as well presence AT-clusters enhances inhibitory effect.
DNA damage caused by the binding of tumorigen 7R,8S-diol 9S,10R-epoxide (B[a]PDE), a metabolite bezo[a]pyrene, to guanine in CpG dinucleotide sequences could affect methylation and, thus, represent potential epigenetic mechanism chemical carcinogenesis. In this work, we investigated impact stereoisomeric (+)- and (−)-trans-anti-B[a]P-N2-dG adducts (B+ B-) on prokaryotic methyltransferases M.SssI M.HhaI. These two recognize GCGC sequences, respectively, transfer methyl group C5 atom cytosine...
Hematogenous metastasis limits the survival of colorectal cancer (CRC) patients. Here, we illuminated roles CD44 isoforms in this process. Isoforms 3 and 4 were predominantly expressed CRC isoform indicated poor outcome correlated with epithelial-mesenchymal transition (EMT) decreased oxidative phosphorylation (OxPhos) patients; opposite associations found for 3. Pan-CD44 knockdown (kd) independently impaired primary tumor formation abrogated distant xenografts. The xenograft tumors mainly...