A5029400117- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Approximation Theory and Sequence Spaces
- Surgical Simulation and Training
- Neuroscience of respiration and sleep
- Mathematical Analysis and Transform Methods
- Molecular Biology Techniques and Applications
- Sparse and Compressive Sensing Techniques
- Hemispheric Asymmetry in Neuroscience
- EEG and Brain-Computer Interfaces
- Advanced Algebra and Logic
- Cancer Cells and Metastasis
- Spinal Cord Injury Research
- Circular RNAs in diseases
- Neural Networks and Applications
- Coding theory and cryptography
- Gene expression and cancer classification
- Technology and Human Factors in Education and Health
- Heart Rate Variability and Autonomic Control
- Neuroendocrine regulation and behavior
- Computational Drug Discovery Methods
- Mathematical Approximation and Integration
- Transcranial Magnetic Stimulation Studies
- Composite Material Mechanics
Moscow State University
2011-2025
Evotec (Germany)
2021-2024
Lomonosov Moscow State University
2013-2022
University of Haifa
2018-2021
BioClinicum (Russia)
2014-2019
National Research University Higher School of Economics
2016-2018
Abstract Background MiRNAs are essential mediators of many biological processes. The aim this study was to investigate the dynamics miRNA-mRNA regulatory networks during exercise and subsequent recovery period. Results Here we monitored transcriptome changes using microarray analysis whole blood eight highly trained athletes before after 30 min moderate followed by 60 We combined expression profiling bioinformatics analysed metabolic pathways enriched with differentially expressed mRNAs...
Quantification and normalization of RT-qPCR data critically depends on the expression so called reference genes. Our goal was to develop a strategy for selection genes that utilizes microarray analysis combines known approaches gene stability evaluation select set appropriate research clinical breast samples with different receptor cancer status using this strategy. A preliminary search based high-throughput datasets. The final validation candidate were several methods evaluation:...
Aberrant sialylation of glycoproteins has been detected in many tumors, and upregulation the beta-galactosamide alpha-2,6-sialyltransferase 1 (ST6GAL1) implicated with tumor aggressiveness chemoresistance experimental models. In our present study, we aimed to study prognostic or predictive role ST6GAL1 ovarian carcinoma, using two independent cancer cohorts. mRNA levels were retrieved from a publicly available database (n = 517), protein analyzed by western blot analysis cohort 204 samples....
We study the generic nature of simplicity, non-affinity and polynomial completeness finite n -quasigroups. It is shown that for fixed almost all -quasigroups are strongly non-affine, i.e., not isotopic to affine Exact number simple, simultaneously simple order 4 established. As a corollary, it proven polynomially complete non-affine.
We propose a homogeneity test closely related to the concept of linear separability between two samples. Using one can answer question whether classifier is merely ``random'' or effectively captures differences classes. focus on establishing upper bounds for test's \emph{p}-value when applied two-dimensional Specifically, normally distributed samples we experimentally demonstrate that bound highly accurate. this bound, evaluate classifiers designed detect ER-positive breast cancer recurrence...
Laminins are a major family of extracellular matrix proteins and the main component basement membranes. involved in many if not all stages cancer progression, expression laminin genes has prognostic value various types cancer, including colorectal. Only single or components trimer with significant differential have been regarded as potential biomarkers to date. Here we compared power classifiers constructed from sets that any gene. The analysis showed cumulative was higher achieved already...
Analysis of regulatory networks is a powerful framework for identification and quantification intracellular interactions. We introduce miRGTF-net, novel tool construction miRNA-gene-TF networks. consider multiple transcriptional post-transcriptional interaction types, including regulation gene miRNA expression by transcription factors, silencing miRNAs, co-expression host genes with their intronic miRNAs. The underlying algorithm uses information on experimentally validated interactions as...
Abstract Background The pattern-forming bacterium Paenibacillus vortex is notable for its advanced social behavior, which reflected in development of colonies with highly intricate architectures. Prior to this study, only two other species ( sp. JDR-2 and larvae ) have been sequenced. However, no genomic data available on the complex motility. Here we report de novo genome sequence Gram-positive, soil-dwelling, sporulating bacterium. Results complete P. was sequenced by a hybrid approach...
Inflammatory breast cancer (IBC) is an extremely malignant form of which can be easily misdiagnosed. Conclusive prognostic IBC molecular biomarkers are also providing the perspectives for targeted therapy lacking so far. The aim this study was to reveal IBC-specific miRNA expression profile and evaluate its association with clinicopathological parameters. profiles 13 17 non-IBC patients were characterized using comprehensive Affymetrix GeneChip 3.0 microarray platform. Bioinformatic analysis...
Abstract Genes with significant differential expression are traditionally used to reveal the genetic background underlying phenotypic differences between cancer cells. We hypothesized that informative marker sets can be obtained by combining genes a relatively low degree of individual expression. developed method for construction highly gene combinations aimed at maximization cumulative power and identified 2–5 efficiently predicting recurrence ER-positive breast patients. The constructed on...
Abstract MicroRNAs (miRNAs) are a family of short noncoding RNAs that posttranscriptionally regulate gene expression and play an important role in multiple cellular processes. A significant percentage miRNAs intragenic, which is often functionally related to their host genes playing either antagonistic or synergistic roles. In this study, we constructed analyzed the entire network intergenic interactions induced by intragenic miRNAs. We further focused on core network, was defined as union...
Mesothelial E- and P-selectins substantially mediate the intraperitoneal spread of Pancreatic ductal adenocarcinoma (PDA) cells in xenograft models. In absence selectins host, integrin subunit alpha-V (ITGAV, CD51) was upregulated remaining metastatic deposits. Here we present first experimental study to investigate if ITGAV plays a functional role PDA tumor growth progression with particular focus on carcinomatosis.Knockdown generated using an RNA interference-mediated approach two cell...
ABSTRACT Paenibacillus dendritiformis is a Gram-positive, soil-dwelling, spore-forming social microorganism. An intriguing collective faculty of this strain manifested by its ability to switch between different morphotypes, such as the branching (T) and chiral (C) morphotypes. Here we report 6.3-Mb draft genome sequence P. C454 morphotype.
Regulation of the formation and rewiring neural circuits by neuropeptides may require coordinated production these signaling molecules their receptors that be established at transcriptional level. Here, we address this hypothesis comparing absolute expression levels opioid peptides with receptors, largest neuropeptide family, characterizing coexpression (transcriptionally coordinated) patterns genes. We demonstrated genes highly correlate within across functionally anatomically different...
Astrocytes are considered to be an important contributor central nervous system (CNS) disorders, particularly multiple sclerosis. The transcriptome of these cells is greatly affected by cytokines released lymphocytes, penetrating the blood-brain barrier-in particular, classical pro-inflammatory cytokine interferon-gamma (IFNγ). We report here transcriptomal profiling astrocytes treated using IFNγ and benztropine, a putative remyelinization agent. Our findings indicate that expression genes...
Finding additional functional targets for combination therapy could improve the outcome melanoma patients. In a spontaneous metastasis xenograft model of human shRNA mediated knockdown L1CAM more than sevenfold reduced number lung metastases after induction subcutaneous tumors two cell lines (MeWo, MV3). Whole genome expression arrays initially high MeWo revealed unchanged or downregulated genes involved in epithelial to mesenchymal transition (EMT) except an upregulation Jagged 1,...
Abstract Mechanisms of motor deficits (e.g. hemiparesis and hemiplegia) secondary to stroke traumatic brain injury remain poorly understood. In early animal studies, a unilateral lesion the cerebellum produced postural asymmetry with ipsilateral hindlimb flexion that was retained after complete spinal cord transection. Here we demonstrate in rats is induced by sensorimotor cortex, characterize this phenomenon as model neuroplasticity underlying asymmetric deficits. After cortical lesion,...
Brain injuries can interrupt descending neural pathways that convey motor commands from the cortex to spinal motoneurons. Here, we demonstrate a unilateral injury of hindlimb sensorimotor rats with completely transected thoracic cord produces postural asymmetry contralateral flexion and asymmetric withdrawal reflexes within 3 hr, as well in gene expression patterns lumbar cord. The injury-induced effects were abolished by hypophysectomy mimicked transfusion serum animals brain injury....
We present a method for evaluating elastic properties of composite material produced by molding resin filled with short fibers. A flow the is simulated numerically using mesh-free method. After that, assuming that spatial distribution and orientation fibers are not significantly changed during polymerization, effective moduli evaluated. The developed micro-mechanical mathematical modelling aimed to process optimization which results in product quality improvement.