A5086447474- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Mycobacterium research and diagnosis
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Immune cells in cancer
- Immune Cell Function and Interaction
- Genomics and Phylogenetic Studies
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Diabetes and associated disorders
- Kruppel-like factors research
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Tendon Structure and Treatment
- Muscle Physiology and Disorders
- IL-33, ST2, and ILC Pathways
- Bacteriophages and microbial interactions
Weizmann Institute of Science
2016-2025
Sheba Medical Center
2012
Hebrew University of Jerusalem
2005-2007
Quark Pharmaceuticals (United States)
2002
Thomas Jefferson University
1994-1995
Hypoxia is an important factor that elicits numerous physiological and pathological responses. One of the major gene expression programs triggered by hypoxia mediated through hypoxia-responsive transcription hypoxia-inducible 1 (HIF-1). Here, we report identification cloning a novel HIF-1-responsive gene, designated RTP801. Its strong up-regulation was detected both in vitro vivo animal model ischemic stroke. When induced from tetracycline-repressible promoter, RTP801 protected MCF7 PC12...
Highlights•m6A/m mRNA methylation in the adult mouse brain is regulated by stress•m6A/m regulation region, time, and gene specific•Mettl3 Fto cKO alter m6A/m, fear memory, expression, synaptic plasticity•The m6A/m glucocorticoid response impaired major depressive disorder patientsSummaryN6-methyladenosine (m6A) N6,2′-O-dimethyladenosine (m6Am) are abundant modifications that regulate transcript processing translation. The role of both, here termed stress vivo currently unknown. Here, we...
Scope Breastfeeding is associated with reduced risk of infection, immune‐mediated disorders, obesity, and even cancer. Recently it was found that breast milk contains a variety microRNAs (miRNAs) in the skim fat layer can be transferred to infants, appear play important roles those biological functions. Methods results This study applied next generation sequencing quantitative real‐time PCR analysis determine miRNA expression profile fraction human, goat, bovine as well infant formulas....
RNA-Seq technology is routinely used to characterize the transcriptome, and detect gene expression differences among cell types, genotypes conditions. Advances in short-read sequencing instruments such as Illumina Next-Seq have yielded easy-to-operate machines, with high throughput, at a lower price per base. However, processing this data requires bioinformatics expertise tailor execute specific solutions for each type of library preparation.In order enable fast user-friendly analysis, we...
The thymus is a primary lymphoid organ that essential for the establishment of adaptive immunity through generation immunocompetent T cells. In response to various stress signals, undergoes acute but reversible involution. However, mechanisms governing its recovery are incompletely understood. Here, we used dexamethasone-induced thymic involution mouse model investigate how hematopoietic cells (excluding cells) contribute regeneration. scRNA-seq analysis revealed marked transcriptional and...
Abstract Speciation via interspecific or intergeneric hybridization and polyploidization triggers genomic responses involving genetic epigenetic alterations. Such modifications may be induced by small RNAs, which affect key cellular processes, including gene expression, chromatin structure, cytosine methylation transposable element (TE) activity. To date, the role of RNAs in context wide has received little attention. In this work, we performed high-throughput sequencing parental, hybrid,...
Summary Fleshy tomato fruit typically lacks stomata; therefore, a proper cuticle is particularly vital for development and interaction with the surroundings. Here, we characterized S l SHINE 3 ( SlSHN3 ) transcription factor to extend our limited knowledge regarding regulation of formation in fleshy fruits. We created overexpressing silenced plants, used them detailed analysis cuticular lipid compositions, phenotypic characterization, study on mode action. Heterologous expression A...
The t(8;21) and inv(16) chromosomal aberrations generate the oncoproteins AML1-ETO (A-E) CBFβ-SMMHC (C-S). role of these in acute myeloid leukemia (AML) etiology has been well studied. Conversely, function native RUNX1 promoting A-E- C-S-mediated leukemias remained elusive. We show that wild-type is required for survival t(8;21)-Kasumi-1 inv(16)-ME-1 leukemic cells. knockdown Kasumi-1 cells (Kasumi-1RX1-KD) attenuates cell-cycle mitotic checkpoint, leading to apoptosis, whereas A-E...
RNA localization is a regulatory mechanism that conserved from bacteria to mammals. Yet, little known about the and logic govern distribution of transcripts within cell. Here, we present novel organ culture system, which enables isolation specifically NGF dependent re-growing peripheral axons mouse embryo, sensory neurons. In combination with massive parallel sequencing technology, determine subcellular most in transcriptome. We found axon enriched mRNAs encode secreted proteins,...
Identification and quantification of small RNAs are challenging because their short length, high sequence similarities within microRNA (miRNA) families, the existence miRNA isoforms O -methyl 3′ modifications. In this study, detection performance three high-throughput commercial platforms, Agilent Affymetrix microarrays Illumina next-generation sequencing, was systematically comprehensively compared. The ability to detect miRNAs shown depend strongly on platform modifications sequence. Using...
The ALLI gene, located at chromosome band 11q23, is involved in acute leukemia through a series of translocations and fusion to variety genes, most frequently A4 AF9. fused genes encode chimeric proteins proteins. Because the Drosophila homologue ALL1, trithorax, positive regulator homeotic acts level transcription, it conceivable that alterations ALL1 transcriptional activity may underlie its action malignant transformation. To begin studying this, we examined All1, AF4, AF9, AF17 for...
The phloem's role as a tissue responsible for the distribution of photoassimilates and nutrients among various organs higher plants has long been recognized. Recent studies have established that numerous proteins mRNA molecules are also present in phloem translocation stream; however, limited information is available on identity transcripts within sap. In this study, genomic approach was taken to produce transcription profile melon A cDNA library constructed from mRNAs extracted sap 1900...
Chromosome region 11q23 is involved in reciprocal chromosome translocations associated with human acute leukemias. These aberrations fuse the ALL-1 gene located at to a series of partner genes positioned on variety chromosomes. The fused encode chimeric proteins. Here we report cloning and characterization 17q21, AF17 gene. encodes protein 1093 amino acids, containing leucine-zipper dimerization motif 3' fusion point cysteine-rich domain N terminus. latter can be arranged three zinc fingers...
Natural killer cells belong to the family of innate lymphoid comprising frontline defense against infected and transformed cells. Development activation natural is highly dependent on interleukin-15 signaling. However, very little known about transcription program driving this process. The factor Runx3 expressed in cells, but its function these largely unknown. We show that loss impaired interleukin-15-dependent accumulation mature vivo under culture conditions pregnant Runx3−/− mice...
Alternative splicing plays a major role in expanding the potential informational content of eukaryotic genomes. It is an important post-transcriptional regulatory mechanism that can increase protein diversity and affect mRNA stability. often regulated tissue-specific stress-responsive manner. Cold stress, which adversely affects plant growth development, regulates transcription factors. This pre-mRNA processing many genes. To identify cold alternative we applied Affymetrix Arabidopsis tiling...
Neuroinflammation is a key phenomenon in the pathogenesis of many neurodegenerative diseases. Understanding mechanisms by which brain inflammation engaged and delineating players immune response their contribution to pathology great importance for identification novel therapeutic targets these devastating Gaucher disease, most common lysosomal storage caused mutations GBA1 gene significant risk factor Parkinson's disease; some forms neuroinflammation observed.An unbiased profile analysis was...
The mechanical challenge of attaching elastic tendons to stiff bones is solved by the formation a unique transitional tissue. Here, we show that murine tendon-to-bone attachment cells are bi-fated, activating mixture chondrocyte and tenocyte transcriptomes, under regulation shared regulatory elements Krüppel-like factors (KLFs) transcription factors. High-throughput bulk single-cell RNA sequencing humeral revealed expression hundreds chondrogenic tenogenic genes, which was validated in situ...
The insulin gene is efficiently expressed only in pancreatic beta cells. Using reverse transcriptase-polymerase chain reaction analysis, we show that mRNA levels are at least 105-fold higher cells than non-beta To examine the underlying mechanisms, cell transcription factors by transfection of Separate expression BETA2, E2A, or PDX1 led to modest (<10-fold) activation promoter, whereas co-expression three proteins produced synergistic, high level (160-fold). This activity ∼25% observed...
Abstract Background The past three decades have witnessed a dramatic increase in interest the whitefly Bemisia tabaci , owing to its nature as taxonomically cryptic species, damage it causes large number of herbaceous plants because specialized feeding phloem, and ability serve vector plant viruses. Among most important viruses be transmitted by B. are those genus Begomovirus (family, Geminiviridae). Surprisingly, little is known about genome this whitefly. haploid size for male has been...