A5080243739- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- NF-κB Signaling Pathways
- Acute Myeloid Leukemia Research
- Mitochondrial Function and Pathology
- Genomics and Chromatin Dynamics
- Pancreatic function and diabetes
- Bacteriophages and microbial interactions
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Immune cells in cancer
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Retinoids in leukemia and cellular processes
- Muscle Physiology and Disorders
- Viral Infections and Immunology Research
- DNA and Nucleic Acid Chemistry
- Down syndrome and intellectual disability research
- Cancer-related gene regulation
- Polyomavirus and related diseases
- Epigenetics and DNA Methylation
- Signaling Pathways in Disease
- Bone Metabolism and Diseases
- Cell Adhesion Molecules Research
Weizmann Institute of Science
2012-2024
Biocenter Finland
2005
University of Helsinki
2005
Institute of Molecular and Cell Biology
2004
Universidad de Navarra
2003
Consejo Superior de Investigaciones Científicas
2003
Centro de Investigaciones Biológicas Margarita Salas
2003
University of Colorado Health
2003
Tel Aviv University
1994-1998
McGill University
1998
The mammalian AML/CBFalpha runt domain (RD) transcription factors regulate hematopoiesis and osteoblast differentiation. Like their Drosophila counterparts, most RD proteins terminate in a common pentapeptide, VWRPY, which serves to recruit the corepressor Groucho (Gro). Using yeast two-hybrid assay, vitro association pull-down experiments, we demonstrate that Gro its homolog TLE1 specifically interact with AML1 AML2. In addition VWRPY motif, other C-terminal sequences are required for these...
Activation of naive CD8+ T cells with antigen induces their differentiation into effector cytolytic lymphocytes (CTLs). CTLs lyse infected or aberrant target by exocytosis lytic granules containing the pore-forming protein perforin and a family proteases termed granzymes. We show that CTL occurs in two sequential phases vitro, characterized early induction T-bet late Eomesodermin (Eomes), T-box transcription factors regulate interferon (IFN) γ expression, respectively. In addition, we...
Down syndrome, the phenotypic expression of human trisomy 21, is presumed to result from a 1.5-fold increase in genes on chromosome 21. As an approach development animal model for several strains transgenic mice that carry Cu/Zn-superoxide dismutase gene have been prepared. These animals express transgene manner similar humans, with 0.9- and 0.7-kilobase transcripts 1:4 ratio, synthesize enzyme active form capable forming human-mouse heterodimers. superoxide activity increased 1.6- 6.0-fold...
The RUNX transcription factors are important regulators of lineage-specific gene expression. bifunctional, acting both as activators and repressors tissue-specific target genes. Recently, we have demonstrated that Runx3 is a neurogenic factor, which regulates development survival proprioceptive neurons in dorsal root ganglia. Here report Runx1 highly expressed thymic medulla cortex, respectively, function CD8 T cells during thymopoiesis. Runx3-deficient (Runx3 KO) mice display abnormalities...
Cytoplasmic superoxide dismutase (SOD-1; EC 1.15.1.1) is encoded by human chromosome 21. The SOD-1 gene locus located at chromosomal region 21q22, which involved in Down syndrome. cDNA clones containing sequences of were previously isolated. In the present study nucleotide sequence one clone, designated pS61-10, was determined. It contains 459 nucleotides representing entire coding and 95 3' untranslated region. cells two poly(A)-containing RNAs 0.7 0.5 kilobases detected. These species are...
Late SV4O 16S and 19S mRNAs were found to contain an average of three m 6 A residues per mRNA molecule. The methylated both the viral cellular occur in two sequences; Gpm ApC (Ap)nm where n = 1–4. More than 60% even though there are twice as many (A) AC GAC sequences these messengers. containing oligonucleotides late SV40 localized In located at 5′ coding region between 0.95–0.0 map units. third residue was mapped 0.0–0.14 nap units translated portion this mRNA. overall pattern internal...
The t(8;21) and inv(16) chromosomal aberrations generate the oncoproteins AML1-ETO (A-E) CBFβ-SMMHC (C-S). role of these in acute myeloid leukemia (AML) etiology has been well studied. Conversely, function native RUNX1 promoting A-E- C-S-mediated leukemias remained elusive. We show that wild-type is required for survival t(8;21)-Kasumi-1 inv(16)-ME-1 leukemic cells. knockdown Kasumi-1 cells (Kasumi-1RX1-KD) attenuates cell-cycle mitotic checkpoint, leading to apoptosis, whereas A-E...
RUNX transcription factors are key regulators of lineage-specific gene expression and might be involved in autoimmune diseases. Runx3 plays a role during the development sensory neurons T cells regulates transforming growth factor β (TGF-β) signaling dendritic cells. Here, we report that at 4 weeks age, knockout (KO) mice spontaneously develop inflammatory bowel disease (IBD) characterized by leukocyte infiltration, mucosal hyperplasia, formation lymphoid clusters, increased production IgA....
Abstract Copper/zinc superoxide dismutase (CuZn‐SOD) is a key enzyme in the metabolism of oxygen free radicals. The gene encoding CuZn‐SOD resides on human chromosome 21 and overexpressed Down syndrome (DS) patients. Overexpression transgenic (Tg) mice cultured cells creates chronic oxidative stress leading to enhanced susceptibility degeneration apoptotic cell death. We have now found that three lines Tg‐CuZn‐SOD mice, one which also overexpresses S100β, glial calcium binding protein, are...
The perichondrium, a structure made of undifferentiated mesenchymal cells surrounding growth plate cartilage, regulates chondrocyte maturation through poorly understood mechanisms. Analyses loss- and gain-of-function models show that Twist-1, whose expression in cartilage is restricted to favors Runx2-dependent manner. Runx2, turn, enhances perichondrial Fgf18 , regulator maturation. Accordingly, compound heterozygous embryos for Runx2 deletion display the same phenotype as -null embryos....
The human chromosome 21 AML1 gene is expressed predominantly in the hematopoietic system. In several leukemia-associated translocations fused to other genes and transcription of regions mediated by upstream sequences that normally regulate expression AML1. 5' genomic region was cloned sequenced. two untranslated (UTRs) previously identified cDNAs were located this distance between them established. distal UTR maps over 7 kb proximal one. Using primer extension with mRNA, start sites at...